Genetic redundancy in iron and manganese transport in the metabolically versatile bacterium<i>Rhodopseudomonas palustris</i>TIE-1

Singh, Rajesh; Ranaivoarisoa, Tahina Onina; Gupta, Dinesh; Bai, Wei; Bose, Arpita

doi:10.1101/2020.05.08.085498

2020

DOI: 10.1101/2020.05.08.085498

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Genetic redundancy in iron and manganese transport in the metabolically versatile bacteriumRhodopseudomonas palustrisTIE-1

Rajesh Singh

Tahina Onina Ranaivoarisoa

Dinesh Gupta

et al.

Abstract: Genetic redundancy in iron and manganese transport in the metabolically 2 versatile bacterium Rhodopseudomonas palustris TIE-1 3 4 Running title: 5 Iron and manganese transport in R. palustris TIE-1 6 7

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Improving bioplastic production byRhodopseudomonas palustrisTIE-1 using synthetic biology and metabolic engineering

Ranaivoarisoa,

Bai,

Rengasamy

et al. 2023

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With the increasing demand for sustainably produced renewable resources, it is important to look towards microorganisms capable of producing bioproducts such as biofuels and bioplastics. Though many systems for bioproduct production are well documented and tested in model organisms, it is essential to look beyond to non-model organisms to expand the field and take advantage of metabolically versatile strains. This investigation centers on Rhodopseudomonas palustris TIE-1, a purple, non-sulfur autotrophic, and anaerobic bacterium capable of producing bioproducts that are comparable to their petroleum-based counterparts. To induce bioplastic overproduction, genes that might have a potential role in the PHB biosynthesis such as the regulator, phaR, and phaZ known for its ability to degrade PHB granules were deleted using markerless deletion. Mutants in pathways that might compete with polyhydroxybutyrate (PHB) production such as glycogen and nitrogen fixation previously created to increase n-butanol production by TIE-1 were also tested. In addition, a phage integration system was developed to insert RuBisCO (RuBisCO form I and II genes) driven by a constitutive promoter PaphII into TIE-1 genome. Our results show that deletion of the phaR gene of the PHB pathway increases PHB productivity when TIE-1 was grown photoheterotrophically with butyrate and ammonium chloride (NH4Cl). Mutants unable to make glycogen or fix dinitrogen gas show an increase in PHB productivity under photoautotrophic growth conditions with hydrogen. In addition, the engineered TIE-1 overexpressing RuBisCO form I and form II produces significantly more polyhydroxybutyrate than the wild type under photoheterotrophy with butyrate and photoautotrophy with hydrogen. Inserting RuBisCO genes into TIE-1 genome is a more effective strategy than deleting competitive pathways to increase PHB production in TIE-1. The phage integration system developed for TIE-1 thus creates numerous opportunities for synthetic biology in TIE-1.

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Improving bioplastic production byRhodopseudomonas palustrisTIE-1 using synthetic biology and metabolic engineering

Ranaivoarisoa,

Bai,

Rengasamy

et al. 2023

Preprint

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Genetic redundancy in iron and manganese transport in the metabolically versatile bacteriumRhodopseudomonas palustrisTIE-1

Abstract: Genetic redundancy in iron and manganese transport in the metabolically 2 versatile bacterium Rhodopseudomonas palustris TIE-1 3 4 Running title: 5 Iron and manganese transport in R. palustris TIE-1 6 7

Cited by 1 publication

References 77 publications

Improving bioplastic production byRhodopseudomonas palustrisTIE-1 using synthetic biology and metabolic engineering

Improving bioplastic production byRhodopseudomonas palustrisTIE-1 using synthetic biology and metabolic engineering

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