Genetic regulation of meiosis in polyploid species: new insights into an old question

Cifuentes, Marta; Grandont, Laurie; Moore, Graham; Chèvre, Anne Marie; Jenczewski, Eric

doi:10.1111/j.1469-8137.2009.03084.x

Cited by 152 publications

(175 citation statements)

References 59 publications

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“…0 94 100 0 100 81 62 100 KCN-10-2 100 100 93 88 36 100 100 100 KCN-10-3 20 78 100 75 21 100 100 100 KCN-10-4 0 44 100 0 28 27 0 0 KCN-10-5 100 94 100 88 100 100 100 Certain B-genome linkage groups appeared to segregate normally in the BC 3 S 1 families, which suggests that they must be forming pairing structures during meiosis, presumably through homeologous interactions. Ten of 16 of the lines analyzed by GISH had even chromosome numbers, a slight bias toward maintaining a balanced nucleus, which can be explained by diploid-like meiotic behavior in allopolyploid species, which is genetically controlled (Cifuentes et al 2010). It has been shown in resynthesized B. napus that the first meiosis promotes a lot of meiotic driven genetic changes and genome rearrangements, which are transmitted to the progeny (Szadkowski et al 2010).…”

Section: Cytological Tracking Of the B-genome Chromosomesmentioning

confidence: 99%

Analysis of B-Genome Chromosome Introgression in Interspecific Hybrids of Brassica napus × B. carinata

et al. 2011

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Brassica carinata, an allotetraploid with B and C genomes, has a number of traits that would be valuable to introgress into B. napus. Interspecific hybrids were created between B. carinata (BBCC) and B. napus (AACC), using an advanced backcross approach to identify and introgress traits of agronomic interest from the B. carinata genome and to study the genetic changes that occur during the introgression process. We mapped the B and C genomes of B. carinata with SSR markers and observed their introgression into B. napus through a number of backcross generations, focusing on a BC 3 and BC 3 S 1 sibling family. There was close colinearity between the C genomes of B. carinata and B. napus and we provide evidence that B. carinata C chromosomes pair and recombine normally with those of B. napus, suggesting that similar to other Brassica allotetraploids no major chromosomal rearrangements have taken place since the formation of B. carinata. There was no evidence of introgression of the B chromosomes into the A or C chromosomes of B. napus ; instead they were inherited as whole linkage groups with the occasional loss of terminal segments and several of the B-genome chromosomes were retained across generations. Several BC 3 S 1 families were analyzed using SSR markers, genomic in situ hybridization (GISH) assays, and chromosome counts to study the inheritance of the B-genome chromosome(s) and their association with morphological traits. Our work provides an analysis of the behavior of chromosomes in an interspecific cross and reinforces the challenges of introgressing novel traits into crop plants.

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Section: Cytological Tracking Of the B-genome Chromosomesmentioning

confidence: 99%

Analysis of B-Genome Chromosome Introgression in Interspecific Hybrids of Brassica napus × B. carinata

et al. 2011

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“…Suppression of COs between homoeologous chromosomes is thus required to ensure fertility. Current understanding is that this process is genetically determined in many allopolyploids and usually subject to polygenic regulation (Jenczewski et al, 2003;Cifuentes et al, 2010). However, it is not known whether recurrent polyploidy drives variation in the determinants of CO between homoeologous chromosomes in allopolyploid species.…”

Section: Introductionmentioning

confidence: 99%

Repeated Polyploidy Drove Different Levels of Crossover Suppression between Homoeologous Chromosomes inBrassica napusAllohaploids

et al. 2010

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Allopolyploid species contain more than two sets of related chromosomes (homoeologs) that must be sorted during meiosis to ensure fertility. As polyploid species usually have multiple origins, one intriguing, yet largely underexplored, question is whether different mechanisms suppressing crossovers between homoeologs may coexist within the same polyphyletic species. We addressed this question using Brassica napus, a young polyphyletic allopolyploid species. We first analyzed the meiotic behavior of 363 allohaploids produced from 29 accessions, which represent a large part of B. napus genetic diversity. Two main clear-cut meiotic phenotypes were observed, encompassing a twofold difference in the number of univalents at metaphase I. We then sequenced two chloroplast intergenic regions to gain insight into the maternal origins of the same 29 accessions; only two plastid haplotypes were found, and these correlated with the dichotomy of meiotic phenotypes. Finally, we analyzed genetic diversity at the PrBn locus, which was shown to determine meiotic behavior in a segregating population of B. napus allohaploids. We observed that segregation of two alleles at PrBn could adequately explain a large part of the variation in meiotic behavior found among B. napus allohaploids. Overall, our results suggest that repeated polyploidy resulted in different levels of crossover suppression between homoeologs in B. napus allohaploids.

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“…Evidence for meiotic gene adaptation to autopolyploidy, involving more than two equivalent pairing partners, has been recently described (Hollister et al, 2012;Yant et al, 2013). In allopolyploids, pairing should be limited to two homologous partners (Comai, 2005;Cifuentes et al, 2010), as opposed to homoeologous partners, which originate from different parents. Strict homologous pairing ensures proper chromosome partitioning and reduces the risks of production of unbalanced gametes, which are often unviable.…”

Section: Introductionmentioning

confidence: 99%

The BOY NAMED SUE Quantitative Trait Locus Confers Increased Meiotic Stability to an Adapted Natural Allopolyploid of Arabidopsis

et al. 2014

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Whole-genome duplication resulting from polyploidy is ubiquitous in the evolutionary history of plant species. Yet, polyploids must overcome the meiotic challenge of pairing, recombining, and segregating more than two sets of chromosomes. Using genomic sequencing of synthetic and natural allopolyploids of Arabidopsis thaliana and Arabidopsis arenosa, we determined that dosage variation and chromosomal translocations consistent with homoeologous pairing were more frequent in the synthetic allopolyploids. To test the role of structural chromosomal differentiation versus genetic regulation of meiotic pairing, we performed sequenced-based, high-density genetic mapping in F2 hybrids between synthetic and natural lines. This F2 population displayed frequent dosage variation and deleterious homoeologous recombination. The genetic map derived from this population provided no indication of structural evolution of the genome of the natural allopolyploid Arabidopsis suecica, compared with its predicted parents. The F2 population displayed variation in meiotic regularity and pollen viability that correlated with a single quantitative trait locus, which we named BOY NAMED SUE, and whose beneficial allele was contributed by A. suecica. This demonstrates that an additive, gain-of-function allele contributes to meiotic stability and fertility in a recently established allopolyploid and provides an Arabidopsis system to decipher evolutionary and molecular mechanisms of meiotic regularity in polyploids.

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Genetic regulation of meiosis in polyploid species: new insights into an old question

Cited by 152 publications

References 59 publications

Analysis of B-Genome Chromosome Introgression in Interspecific Hybrids of Brassica napus × B. carinata

Analysis of B-Genome Chromosome Introgression in Interspecific Hybrids of Brassica napus × B. carinata

Repeated Polyploidy Drove Different Levels of Crossover Suppression between Homoeologous Chromosomes inBrassica napusAllohaploids

The BOY NAMED SUE Quantitative Trait Locus Confers Increased Meiotic Stability to an Adapted Natural Allopolyploid of Arabidopsis

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