Genetic Relatedness among the Indigenous Isolates of Trichoderma harzianum, using RAPD and their Nematocidal Capabilities against Meloidogyne javanica

Khattak, Baharullah; Saifullah, Saifullah; Hussain, Shaukat; Ahmad, Musharraf; Ali, Asad; Junaid, Mohammad; Khan, Ijaz Ahmad; Khan, Taj Ali; Hussain, Mubbashir

doi:10.17582/journal.sja/2018/34.2.486.493

Cited by 2 publications

(4 citation statements)

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Section: Resultsmentioning

confidence: 99%

“…In our work, the UPGMA dendrogram illustrated that T. asperellum isolates could not be grouped by their lytic enzymes production and/or antifungal activity. Previous studies employed the RAPD marker to evaluate the genetic relationships between different Trichoderma isolates and the results indicated high level of polymorphisms [39][40][41]69,70]. In this experiment, Trichoderma strains were grown on two growth media (PDA and CMD) and incubated at two temperatures to estimate their ability to grow under thermal stress.…”

Section: Rapd Analysis Of Trichoderma Strainsmentioning

confidence: 99%

“…In particular, molecular DNA markers are employed as a reliable tool to determine the genetic diversity and relationships [ 34 , 35 ]. RAPD is one of the valuable markers which has been utilized to determine the biodiversity of Trichoderma species, due to its use in identifying mysterious genomes and its requirement of only limited quantities of DNA [ 36 , 37 , 38 , 39 , 40 , 41 ]. SSR has likewise been proved to be a robust marker to investigate polymorphism and discriminate between different species of Trichoderma [ 42 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Hewedy

Lateif

Seleiman

et al. 2020

Biology

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Trichoderma species are known as excellent biocontrol agents against soil-borne pathogens that cause considerable crop losses. Eight strains of Trichoderma were isolated from five Egyptian regions. They identified based on translation elongation factor-1α (TEF1) sequencing as four different Trichoderma species: Trichoderma asperellum, Trichoderma harzianum, Trichoderma viride, and Trichoderma longibrachiatum. Optimal growth conditions (temperature and media), and the phosphate solubilization capability of Trichoderma strains were evaluated in vitro. Further, the ability of these strains to antagonize Fusarium solani, Macrophomina phaseolina, and Fusarium graminearum was also evaluated. The results revealed that Trichoderma harzianum (Th6) exhibited the highest antagonistic ability against F. solani, M. phaseolina and F. graminearum with inhibition rates of 71.42%, 72.97%, and 84.61%, respectively. Trichoderma viride (Tv8) exhibited the lowest antagonism against the same pathogens with inhibition rates of 50%, 64% and 69.23%, respectively. Simple-sequence repeats (SSRs) and random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic variability of the Trichoderma strains. The results revealed that of 45 RAPD amplified bands, 36 bands (80%) were polymorphic and of SSRs amplified 36 bands, 31 bands (86.11%) were polymorphic. The amplification of calmodulin and β-1,3-endoglucanase was noted at 500 bp and 230 bp, respectively. Data indicated that T. viride (Tv8) had the highest phosphate solubilization index (10.0 mm), while T. harzianum (Th6) had the lowest phosphate solubilization index (4.0 mm). In conclusion, T. harzianum (Th6) had the highest antagonistic activity in dual culture assay along with the growth rate; while T. viride (Tv8) had the highest phosphate solubilization activity. There are still gaps in obtaining new formulations, selecting potent Trichoderma strains to confirm disease control in planta. For improving Trichoderma recommendation in the organic agricultural system and sustaining the fertility of the soil, the field application of highly antagonistic biocontrol agents in different types of soil and plant species will be the first approach toward bio-pesticide treatments along with bio-fertilizer inoculation. Furthermore, secondary metabolites will be investigated for the most promising strains with the combination of different pathogens and application timing.

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Section: Resultsmentioning

confidence: 99%

Section: Rapd Analysis Of Trichoderma Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Hewedy

Lateif

Seleiman

et al. 2020

Biology

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“…En la actualidad, los marcadores moleculares son ampliamente utilizados para el estudio de relaciones taxonómicas y diversidad genética en disímiles organismos, incluyendo los hongos (El_Komy et al, 2015). Los RAPD (del inglés Random Amplified Polymorphic DNA, Polimorfismo del ADN Amplificado al Azar) son de gran utilidad en la detección de polimorfismo intra e interespecífico en hongos, debido a que la técnica produce perfiles específicos individuales de ADN molde, mediante la amplificación de fragmentos aleatorios que se distribuyen por todo el genoma (Khattak et al, 2018). Esto ha permitido su utilización en el mapeo, como la generación de huellas genéticas (Hassan et al, 2019).…”

Section: Introductionunclassified

Caracterización morfo – cultural y variabilidad genética y molecular de aislamientos de Trichoderma

Ynfante Martínez,

Martínez-Coca,

Peteira-Delgado

et al. 2023

BIOTECNIA

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El trabajo tuvo como objetivo caracterizar aislados de Trichoderma sobre la base de caracteres morfo-culturales, compatibilidad vegetativa y variabilidad molecular. Las descripciones morfológicas se realizaron a partir de observaciones microscópicas de microcultivos, según Rifai, Gams y Bissett. Las relaciones de compatibilidad vegetativa se evaluaron macroscópicamente y se determinó el tipo de reacción (compatible e incompatible). La variabilidad genética de los aislamientos se determinó mediante el uso de la técnica RAPD; los resultados se analizaron por el método Jaccard mediante el paquete estadístico FreeTree. Los aislados presentaron características morfológicas similares, no obstante, mostraron diferencias en la coloración de las colonias y la morfometría de las estructuras fúngicas. Los aislamientos mostraron compatibilidad vegetativa con las especies Trichoderma viride, Trichoderma asperellum y Trichoderma atroviride, como entre ellos, lo que muestra la cercanía genética. Los 11 iniciadores RAPD generaron un total de 92 bandas reproducibles. De estas, 65 fueron polimórficas, para un 70,7 % de polimorfismo; solo OPH-19 mostró 100 % de polimorfismo. El análisis de agrupamiento por UPGMA mostró variabilidad intraespecífica, formándose cuatro grupos. Para T.13, T.17, T.75 y T.78 se detectaron bandas específicas, importante para el diseño de cebadores específicos, lo que posibilita su autenticación, protección y monitoreo en sistemas productivos.

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Genetic Relatedness among the Indigenous Isolates of Trichoderma harzianum, using RAPD and their Nematocidal Capabilities against Meloidogyne javanica

Cited by 2 publications

References 0 publications

Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Caracterización morfo – cultural y variabilidad genética y molecular de aislamientos de Trichoderma

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