Genetic relatedness between methicillin-susceptible and methicillin-resistant Staphylococcus aureus: results of a national survey

Hallin, Marie; Denis, Olivier; Deplano, Ariane; Mendonça, Ricardo De; Ryck, R. De; Rottiers, S.; Struelens, Marc

doi:10.1093/jac/dkl535

Cited by 76 publications

(81 citation statements)

References 42 publications

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“…they cannot be distinguished by currently used typing procedures. Interestingly, according to MLST, most MRSA clones that frequently are associated with nosocomial infections today also were predominant among MSSA isolates recovered during the 1960s from hospitals in Denmark (14) and more recently in England (15) and Belgium (16). Similar results were obtained in a carriage study from the United States by DNA macrorestriction analysis (17).…”

supporting

confidence: 63%

Frequent emergence and limited geographic dispersal of methicillin-resistant Staphylococcus aureus

Nübel

Filloux

Feldkamp

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

242

181

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A small number of clonal lineages dominates the global population structure of methicillin-resistant Staphylococcus aureus (MRSA), resulting in the concept that MRSA has emerged on a few occasions after penicillinase-stable ␤-lactam antibiotics were introduced to clinical practice, followed by intercontinental spread of individual clones. We investigated the evolutionary history of an MRSA clone (ST5) by mutation discovery at 108 loci (46 kb) within a global collection of 135 isolates. The SNPs that were ascertained define a radial phylogenetic structure within ST5 consisting of at least 5 chains of mutational steps that define geographically associated clades. These clades are not concordant with previously described groupings based on staphylococcal protein A gene (spa) typing. By mapping the number of independent imports of the staphylococcal cassette chromosome methicillin-resistance island, we also show that import has occurred on at least 23 occasions within this single sequence type and that the progeny of such recombinant strains usually are distributed locally rather than globally. These results provide strong evidence that geographical spread of MRSA over long distances and across cultural borders is a rare event compared with the frequency with which the staphylococcal cassette chromosome island has been imported.antibiotic resistance ͉ evolution ͉ phylogeography ͉ SNP

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supporting

confidence: 63%

Frequent emergence and limited geographic dispersal of methicillin-resistant Staphylococcus aureus

Nübel

Filloux

Feldkamp

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

242

181

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“…Analysis of the relationship among SCCmec and toxin genotypes and toxin genes encoding profiles of mobile genetic elements has a possible role in determining superantigenic toxin genotypes in S. aureus. Since frequent horizontal transfer of SCCmec and other mobile genetic elements seem to be the source of the ongoing genetic diversification of MRSA (Robinson and Enright 2003;Lindsay et al 2006;Hallin et al 2007), comparative prevalence studies of large MRSA populations should be conducted in parallel with MRSA surveillance studies, to understand how natural populations of MSSA and MRSA co-evolve and interact.…”

Section: Discussionmentioning

confidence: 99%

“…Staphylococcus aureus is a leading cause of human disease in the hospital setting as well as in the community accounting for a wide range of diseases from superficial skin infections to life-threatening conditions such as bacteremia, endocarditis, pneumonia and toxic shock syndrome (Lowy 1998;Hallin et al 2007). Infections with S. aureus are especially difficult to treat because of evolved resistance to antimicrobial drugs.…”

mentioning

confidence: 99%

Superantigenic Toxin Genes Coexist with Specific Staphylococcal Cassette Chromosome mec Genes in Methicillin-Resistant Staphylococcus aureus

Maina

Omoe

et al. 2011

Tohoku J. Exp. Med.

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Staphylococcus aureus is a leading cause of human disease in the hospital setting and the community. Superantigenic toxin-producing methicillin-resistant Staphylococcus aureus (MRSA) is currently important for nosocomial infections and food-borne diseases worldwide because of its global spreading and difficulty in therapy. Superantigenic toxins can bypass normal antigen presentation and have strong T cell mitogenic activity, leading to massive release of proinflammatory cytokines and contributing to the severity of S. aureus sepsis. In this study, a total of 131 MRSA isolates from patients in the University Hospital were searched for staphylococcal cassette chromosome mec (SCCmec) genes and the staphylococcal superantigenic toxin genes by multiplex polymerase chain reactions. The MRSA isolates were classified into SCCmec type II (74.8%), type I (13.0%), type IV (3.8%), type V (2.3%), and type I and type II (3.8%). MRSA isolates (102/131) also carried a number of superantigenic toxin genes including staphylococcal enterotoxin (se) and toxic shock syndrome toxin-1 (tst-1) genes. The most frequent superantigen gene profile (55/131, 42.0%) of the MRSA isolates includes staphylococcal enterotoxin C (sec), seg, sei, staphylococcal enterotoxin-like L (sell), selm, seln, selo, and tst-1. Furthermore, SCCmec type I or type II MRSA isolates more frequently harbor sec, seg, sei, sell, selm, seln, selo, and tst-1 genes, compared to other types of MRSA. These results indicate that the selected superantigenic toxin genes are linked to SCCmec type I and type II. The coexistence of these toxins and the SCCmec genes in S. aureus may contribute to the biological fitness and pathogenicity of MRSA.

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“…Staphylococcus aureus is an important enterotoxin-producing causative agents accounting for food poisoning, which can also be the pathogen responsible for some other diseases, such as bacteraemia, endocarditis, pneumonia and toxic shock syndrome and so on ( Hallin et al, 2007;Hu et al, 2008;Hsieh et al, 2008). It has been described previously that most S. aureus strains produce one or more groups of specific exoproteins, which include staphylococcal enterotoxins (SE), staphylococcal exofoliative toxins (ET), and toxic shock syndrome toxin 1 (TSST-1) (Balaban et al, 2000;Thomas et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis and antibiotic resistance investigation of Staphylococcus aureus isolates associated with staphylococcal food poisoning and nosocomial infections

Zhang¹,

SuYun²,

Ding³

et al. 2011

Afr. J. Biotechnol.

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Enterotoxins (SEs) generated by Staphylococcus aureus were considered to be the main reason for staphylococcal food-poisoning (SFP). The aim of this research is to investigate the distribution difference of enterotoxin genes in strains isolated from food, food poisoning, inpatients, respectively. Multiplex polymerase chain reaction (PCR) was employed to detect SEs and relationship between antibiotic resistance and living conditions of S. aureus has also been analyzed. Pulsed-Field Gel Electrophoresis (PFGE) was further applied for the determination of the genetic relationships between S. aureus isolates. It was striking to found that positive detection ratio of multi-enterotoxin genes and newly found enterotoxin genes in inpatients' isolates were higher than the other two (P<0.01). SEG, SEI, SEK, SEQ genes were mainly presented in isolates from inpatients while, SEA, SEE, SHE, SEQ genes mainly presented in isolates from food samples and SEA, SEP, SEC genes mainly found in strains from food poisoning. The most important fact was that percentages of isolates from inpatient resistant to oxacillin were 71.43%, which was significantly higher than the percentages (2.33% and 2.38%) of the other two original isolates. The similarity among these isolates was above 70% and PFGE was agreed to be a useful discriminating typing method for S. aureus isolates.

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Genetic relatedness between methicillin-susceptible and methicillin-resistant Staphylococcus aureus: results of a national survey

Cited by 76 publications

References 42 publications

Frequent emergence and limited geographic dispersal of methicillin-resistant Staphylococcus aureus

Frequent emergence and limited geographic dispersal of methicillin-resistant Staphylococcus aureus

Superantigenic Toxin Genes Coexist with Specific Staphylococcal Cassette Chromosome mec Genes in Methicillin-Resistant Staphylococcus aureus

Molecular analysis and antibiotic resistance investigation of Staphylococcus aureus isolates associated with staphylococcal food poisoning and nosocomial infections

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