Genetic Relationship Among Ten Promising Eggplant Varieties Using RAPD Markers

Abstract

RAPD technique was used as a tool for assessing genetic diversity and varietal relationships among ten varieties of eggplant. Out of 21 primers screened four were selected. With these primers 76 clear and bright fragments were obtained of which 44 fragments considered polymorphic. The proportion of polymorphic loci and gene diversity values across all loci were 57.89% and 0.23, respectively. The UPGMA dendrogram based on genetic distance segregated the ten varieties of eggplant into two main clusters. Dohazari, Kazla, Nayantara and ISD-006 were grouped together in cluster I whereas Uttara, Islampuri, Khatkhatia, Singnath, BARI Begun-08 and Eggplant Line-083 into cluster II. Kazla and Nayantara variety pair was very close to each other with the highest intervarietal similarity index (92.54%) and lowest genetic distance (0.14). On the other hand, Khatkhatia and Nayantara pair was the lowest intervarietal similarity index (41.67%) with highest genetic distance (0.48). Therefore, identification of genetically distinct varieties using RAPD markers could be a potential tool for eggplant improvement.

Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

“…Relationship among accession could be explored by quantitative morphological characters (Chernet, Belew, & Abay, 2014). With the improvement of DNA technology, assessment of genetic relationship have been done by many molecular markers (Biswas, Akhond, Al-Amin, Khatun, & Kabir, 2009;Chen et al, 2009;Pu et al, 2009;Comlekcioglu, Simsek, Boncuk, & Aka-Kacar, 2010;Meng, Xu, Huang, & Li, 2010;Reck-Kortmann, Rodrigues, Ruas, & Freitas, 2013;Sharifova, Mehdiyeva, Theodorikas, & Roubos, 2013;Tabassum, Sony, Copyright © 2017 Universitas Brawijaya Bhajan, & Islam, 2013;Tasma, Warsun, Satyawan, Syafaruddin, & Martono, 2013;Aida & Eltayeb, 2015) Other researchers used the combination of both morphology and molecular makers (Paul, Bandyophadyay, Acharyya, & Raychaudhuri, 2010;Naz, Zafrullah, Shahzadhi, & Munir, 2013).…”

“…RAPD markers are more effective and efficient to determine the recombinant variability than morphological markers in assessing genetic diversity (Paul, Bandyophadyay, Acharyya, & Raychaudhuri, 2010;Hapsoro, Warganegara, Utomo, Sriyani, & Yusnita). RAPD markers also become a potential tool to identify genetic differences among varieties (Biswas, Akhond, Al-Amin, Khatun, & Kabir, 2009). Only in a few cases morphological characteristics can be attributed to local adaptation and appropriate genetic variations (Reck-Kortmann, Rodrigues, Ruas, & Freitas, 2013).…”

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

“…Thirty RAPD decamer primers from the Operon kit (Operon Technology, OPT 1-20 and OPF 1-10) and 33 ISSR primers of the UBC primer set were used. The other ISSR primer sets were screened according to Raina et al (2001), Bornet et al (2002), Mariana et al (2006), and Biswas et al (2009). The amplification of RAPD was performed with initial denaturation at 94°C for 2 min, 39 cycles of denaturating at 94°C for 1 min, annealing at 37°C for 1 min and extension at 72°C for 2 min, and a final extension at 72°C for 12 min in a thermocycler (Applied Biosystem 9700).…”

“…Furthermore, the identification of interspecific hybrids relies on both morphological traits and molecular markers. If the hybrid is morphologically similar to one of its parents, molecular markers such as inter-simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) are necessary to help in progeny identification (Ilbi, 2003;Yonemoto et al, 2006;Isshiki et al, 2008;Biswas et al, 2009;Demir et al, 2010). RAPD and ISSR markers have revealed polymorphic DNA bands that differ between genotypes of strawberry.…”

SSR and RAPD-Based Genetic Diversity in Cotton Germplasms

“…A diverse level of polymorphism in different crops have been reported earlier such as Chickpea 98.14% (Rasool 2013), Brassica 98.03% (Ghosh et al 2009), Eggplant 57.89% (Biswas et al 2009) and Chilli 90% (Paran et al 1998). A wide range of RAPD polymorphism in cotton germplasms was also reported.…”