Genetic Relationship amongSome Wheat Genotypes Using Ten ISSR Markers

Ghufaili, Melathkadem FarhoodAL; Al-Tamimi, Attyaf Jameel Thamir

doi:10.9790/3008-1203063036

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…The highest number of primary and polymorphic bands was 18 and 10, respectively, in primer OPA-10. Recognition of the highest number of annealing sites by primer usually results in a high number of main bands, also established and reported in past studies in maize (Al-Tamimi and Al-Saadi 2018), in wheat (Tahir, 2014;Al-Ghufaili, 2017), and that later enhances the chances to provide polymorphic bands.…”

Section: Resultssupporting

confidence: 71%

Assessment of Variations in Catharanthus Roseus L. Induced by Gamma Rays and Sodium Azide Using Rapd Markers

AL-MUSAWI¹,

AL-TAMIMI²

2023

SABRAOJBG

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The study of genetic variations in Catharanthus roseus L. induced by gamma rays and sodium azide using RAPD markers transpired in 2021–2022 at the Department of Biology, University of Kufa, Iraq. The seeds of two cultivars of Catharanthus roseus L., i.e., victory pure white and local (pink variety), received two doses of gamma rays (50 and 75 Gray) and four sodium azide concentrations (0.2%, 0.4%, 0.6%, and 0.8%). Non-treated seeds of both cultivars also served as a control for comparison. Evaluating the effects obtained through the exposure of C. roseus seeds to gamma rays and sodium azide employed DNA markers, including 16 primers used in applying RAPDs. DNA extraction, accomplished from fresh apical leaves, underwent molecular markers application. RAPD markers could detect the generation of genetic variation induced by gamma rays and sodium azide treatments through the appearance of polymorphic bands. Using 16 RAPD primers, the largest molecular size was 2,543 bp produced by primer OPB-17, with the lowest 114 bp produced by primer OPC-08. The highest number of main and polymorphic bands was 18 and 10, respectively, in primer OPA-10. The highest number of monomorphic bands was eight in primer OPX-17, which affected its ability to give the lowest value for polymorphism. The highest number of amplified bands was 78 bands in primer OPC-09. Primer OPA-10 provided the highest number of polymorphic and unique bands, as well as, the highest values for discrimination and primer efficiency. Primer OPC-09, with seven treatments resulting in a unique fingerprint, is superior to all other primers.

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Section: Resultssupporting

confidence: 71%

Assessment of Variations in Catharanthus Roseus L. Induced by Gamma Rays and Sodium Azide Using Rapd Markers

AL-MUSAWI¹,

AL-TAMIMI²

2023

SABRAOJBG

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“…Among DNA markers, ISSR and RAPD require small quantities of DNA and do not require radioactive labels; they are simple and fast. These markers are reproducible, powerful, simple, quick, and relatively cheap for use in estimating genetic variation and identifying differences between populations [11,13,28]. In the present study, both RAPD and ISSR markers generated reproducible and distinct banding patterns.…”

Section: Discussionmentioning

confidence: 55%

Ultrastructure Traits and Genetic Variability of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) Adults from Different Geographical Locations in Egypt

et al. 2022

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The Red Palm Weevil (RPW) is one of the most damaging pests to palm cultivation; this invasive weevil poses a threat to the palm industry. The characterization and identification of this pest in order to determine its biological diversity is the first step in controlling it, which will help in developing effective control programs. The purpose of this study is to investigate the biodiversity of and characterize RPW from five different Egyptian geographical locations at morphological and genetic levels using morphometric analysis, scanning electronic microscopy and two different genetic markers. Our results revealed no significant differences between length and width of the adult body among RPW adults from different geographical locations. Different typologies of prothoracic spots were observed, indicating a degree of diversity in the RPW populations. The magnitude of the different body parts was measured among both males and females. Significant differences were exhibited between length of the antennal seta, as well as forelegs, the lengths and widths of the pronotum, and the rostrum length between both sexes. Both RAPD and ISSR used DNA markers, generating reproducible and distinct banding patterns. The polymorphic banding patterns that have resulted from all studied populations confirmed that these markers demonstrate genetic variability amongst the studied Egyptian populations of Rhynchophorus ferrugineus. The recorded differences may be due to the presence of different red palm weevil genotypes. The obtained results might have potential applications in developing a new tracking and control strategy for this invasive pest.

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“…,since all treatments belong to coriander , appearance related to this relationship (Al-Judy.,2004) because genome contains conserved sequence retained in all treatments including those arises from irradiation treatments) ,these shared sequences appeared in form of monomorphic bands (Russel et al, 1997;,AL-Badeiry, 2013 andAl-Tamimi,2020). Primer OPA-10 gave highest number unique bands ( reached to seven bands ) ,primer ability of to recognize a unique annealing site on genome success in producing a unique DNA fingerprint for a particular genotype (Fadoul et al,2013 ;AL-Ghufaili, 2017, AL-Saadi.,2018,AL-Tamimi.,2021. Primer OPC-19 gave highest value for amplified bands , primer OPA-10 gave highest value for main bands ,these two criteria concerned primer ability to recognize more annealing sites.…”

Section: Resultsmentioning

confidence: 99%

Mutation induced by gamma irradiation in coriander seeds and their identification by RAPD makers

Jabbar

Al-Tamimi

2022

ijhs

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This study was accomplished to detect variation generated through exposure of Coriander ( Coriandrum sativum L.) seeds to five gamma irradiation doses (0,25,50,100 and150 ) GY by using eighteen RAPDs primers as molecular marker ,results indicated that primers OPC-05 ,OPC-19, OPA-10,OPA-15 and OPB-06 success in giving three treatments a unique fingerprint .Molecular size of amplified ranged between 1860 bp produced by primer OPA-04 and 115 bp produced by primer OPW-04 . Highest degree for polymorphism was 91.66 % produced by primer OPA-04. Monomorphic bands appeared in their higher value in primer OPC-09 . Primer OPA-10 gave highest number unique bands ( reached to seven bands ) Primer OPC-19 gave highest value for amplified bands , primer OPA-10 gave highest value for main bands, Discrimination, in addition to primer efficiency value produced in their high value by primer OPA-04.This study revealed RAPD markers in detection mutation induced by gamma irradiation.

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Genetic Relationship amongSome Wheat Genotypes Using Ten ISSR Markers

Cited by 4 publications

References 14 publications

Assessment of Variations in Catharanthus Roseus L. Induced by Gamma Rays and Sodium Azide Using Rapd Markers

Assessment of Variations in Catharanthus Roseus L. Induced by Gamma Rays and Sodium Azide Using Rapd Markers

Ultrastructure Traits and Genetic Variability of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) Adults from Different Geographical Locations in Egypt

Mutation induced by gamma irradiation in coriander seeds and their identification by RAPD makers

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