Genetic Relationship between Thirteen Genome Types of Adenovirus 11, 34, and 35 with Different Tropisms

Li, Quan-Gen; Hambraeus, Johan; Wadell, Göran

doi:10.1159/000150218

Cited by 49 publications

(50 citation statements)

References 12 publications

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“…12-14 Ad35 is not widespread in the general population. 14,15 Although the partial sequence of the Ad35 genome covering the E3 region has been published, 16 in this report we describe the complete 34 794 base pair (bp) sequence of Ad35, the analysis of the open reading frames (ORFs), and the comparison with other previously characterized adenoviral serotypes. Furthermore, we have developed E3-, E1-, and E1/E3-deleted Ad35-based vectors and verified their ability to efficiently infect both human and rhesus macaque dendritic cells (rhDC).…”

Section: Introductionmentioning

confidence: 99%

Human adenovirus type 35: nucleotide sequence and vector development

2003

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In this report, we describe the complete 34 794 base pair genomic sequence of the human adenovirus serotype 35 (Ad35) Holden strain. The viral genome exhibits a compact organization similar to other adenoviral serotypes, with overlapping genes on both strands. In all, 47 open reading frames (ORFs) were identified, including early (E1, 2, 3, 4) and late (L1, 2, 3, 4, 5) regions conserved among the adenoviridae family. In addition, 14 ORFs were identified that do not encode known adenoviral genes. Comparison of the predicted translational products of the conserved genes with those of other adenoviruses revealed that Ad35 has high homology to Ad7, Ad3, Ad21, Ad17, and simian Ads25. Based on the complete Ad35 DNA sequence, E3-, E1-, and E1/E3-deleted Ad35-based vector systems were developed.An HEK293-derived cell line was established for the propagation of the E1-deleted Ad35 vector, avoiding the emergence of replication-competent adenovirus. Moreover, production of the E1-deleted recombinant Ad35 vector was achieved by transient transduction of a plasmid encoding the Ad35 E1B gene in HEK293 cells. Testing showed that the Ad35-based vector efficiently infects both human and rhesus macaque dendritic cells. Our novel Ad35-based vectors and their corresponding packaging cell lines will provide a versatile and powerful system for DNA-based vaccine development and gene therapy applications.

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Section: Introductionmentioning

confidence: 99%

Human adenovirus type 35: nucleotide sequence and vector development

2003

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“…Analysis of an earlier described "HAdV-B11a" hexon (GenBank accession no. AY972815) (2) shows it is identical to the one embedded in the "QS" genome (23), suggesting that "HAdVB11a" was incorrectly characterized earlier as a variant of HAdV-B11 (2,6,11,22) by the use of "then-available" assays. Serum neutralization has the hexon as its target, as does limited molecular typing (3,13).…”

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confidence: 99%

“…Since genome rearrangements are missed by serological and molecular assays against a limited repertoire, it is problematic to rely solely on these methods to characterize a virus thoroughly. Thus, due to the limitations of these assays, the original "HAdV-B11a" isolates have been misnamed as well (2,6,11).…”

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confidence: 99%

“…HAdV-B11 is a member of subspecies B2 and was not originally associated with respiratory disease (14); only HAdV-B14 in this group is associated with respiratory disease (20). A clue as to the incorrect association of HAdV-B11 as a respiratory pathogen lies in the reports that "HAdV-B11a" was unusual in having cellular tropism to respiratory epithelial cells rather than renal cells, as observed for original and subsequent isolates of HAdV-B11 (11,23). In addition, the original "HAdVB11a" did not agglutinate monkey erythrocytes, unlike the true HAdV-B11 field strains and the prototype Slobitski strain (5).…”

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Computational Analysis Identifies Human Adenovirus Type 55 as a Re-Emergent Acute Respiratory Disease Pathogen

et al. 2010

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“…Genomic homology between two strains was calculated using the percentage of pairwise comigrating restriction fragments (% PCRF) which corresponds to the comigrating restriction fragments of a pair divided by the total number of bands of the pair. 7 A numerical code for each restriction profile was attributed in the chronological order of strain isolation to separate genomic variants. A non-epidemiologically related adenovirus 8 strain (kindly provided by Professor Aymard, Faculté de Médecine, Lyon, France) was included in the analysis as an outgroup.…”

Section: -5mentioning

confidence: 99%

Molecular epidemiology of ocular isolates of adenovirus 8 obtained over nine years.

et al. 1999

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Twenty nine strains of adenovirus 8 have been isolated over nine years in Strasbourg, France, 22 of which were from one private ophthalmologist. To assess a possible relation between these strains, the DNA of adenovirus was analysed by restriction fragment length polymorphism using eight diVerent enzymes. Among these, three proved discriminant (Xba I, Bgl II, Eco RI) and made it possible to define 13 genotypes diVering from each other by one to three DNA bands. Seven genotypes were unique isolates, while three, representing 16 strains, were isolated over five to eight years. All the genotypes but one were closely related, with 87% homology. All 13 diVered from an adenovirus 8 strain from Lyon (homology 68-76%). This study confirmed the stability of adenovirus 8 in a given population. (J Clin Pathol 1999;52:860-861) Keywords: adenovirus 8; molecular epidemiology; eye isolates Adenovirus 8 is a common cause of epidemic keratoconjunctivitis that may be transmitted by ocular examination apparatus. These infections are without any long term consequences on eye function. The epidemiology of adenoviruses is currently under investigation using restriction fragment length polymorphism (RFLP) of the entire adenovirus genome. This allows identification of genomic variants (genotypes) and analysis of their distribution in time and space. 1-5Methods From 1989 to 1997, 29 strains of adenovirus 8 have been isolated in the virology laboratory, Faculty of Medicine, Strasbourg, France. Twenty two strains were from one private ophthalmologist, three from another private specialist, and four from diVerent units of the Strasbourg University Hospital.All the strains were isolated on human diploid fibroblasts, MRC-5, serotyped by seroneutralisation or immunofluorescence with a monoclonal antibody at the time of isolation, and stored at −80°C. For RFLP analysis, viruses were grown on MRC-5 cells. The intracellular viral DNA was extracted according to the method of Shinagawa et al 6 modified by Li et al. 7 Briefly, infected cells were recovered in a 50 ml tube, pelleted (1500 g, 15 minutes, 4°C), and suspended in 1.5 ml of TE buVer (10 mM Tris-HCl, 1 mM EDTA, pH 7.9). Cells were lysed by 0.01% sodium dodecyl sulphate (SDS) in 1 M NaCl overnight at 4°C. The cellular debris was pelleted (17 500 g, 30 minutes, 4°C) and the virus containing supernatant was extracted with an equal volume of phenol. The phenolic phase was extracted twice with equal volumes of TE buVer, after which 1.5 volumes of 95% ethanol were added to the aqueous phase and left overnight at −20°C. After centrifugation (1500 g, 15 minutes, 4°C) the pellet was suspended in 500 µl of lysis buVer (0.5% SDS, 100 mM NaCl, 50 µg/ml proteinase K) and incubated at 37°C for about one hour. After two successive extractions with equal volumes of phenol and ether, the aqueous phase was incubated with an equal volume of isopropanol overnight at −20°C. After centrifugation (17 000 g, 20 minutes, 4°C), the pellet of purified adenovirus DNA was dissolved in 100 µl of TE buV...

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Genetic Relationship between Thirteen Genome Types of Adenovirus 11, 34, and 35 with Different Tropisms

Cited by 49 publications

References 12 publications

Human adenovirus type 35: nucleotide sequence and vector development

Human adenovirus type 35: nucleotide sequence and vector development

Computational Analysis Identifies Human Adenovirus Type 55 as a Re-Emergent Acute Respiratory Disease Pathogen

Molecular epidemiology of ocular isolates of adenovirus 8 obtained over nine years.

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