Genetic Relationship of Four Strains of Guppy (Poeciliareticulata Peters, 1859) Using RAPD-PCR Method

Abstract

This study aims to determine the genetic relationship between four strains of guppy, albino full platinum (AFP), albino german yellow (AGY), top sword (TS) and guppy yellow cobra (GYC) using the RAPD-PCR method. This study used explorative method without experimental design and analyzed by descriptive qualitative and quantitative. The obtained genetic relationship data could be used as data reference for hybridization between strains of guppy fish that have been researched. The research was conducted in October 2020-April 2021. The three fish samples (AFP, TS and GYC) obtained from fish breeder in Cilengkrang-Bandung and AGY sample obtained from fish breeder in Tanggerang-Banten. Based on the results of amplification using OPA-03 primer (AGTCAGCCAC), four strains of guppy fish showed 30 DNA bands that included polymorphic and monomorphic bands. The AFP strains had 19 monomorphic bands, AGY had 21 DNA bands (20 monomorphic bands and one polymorphic bands), TS had 19 DNA bands (17 monomorphic bands and two polymorphic bands) and GYC had 15 DNA bands (14 monomorphic bands and one polymorphic band). Phylogenetic tree analyzed by NTSys program. It is shown between AFP and AGY strains had 95% relationship index, then between TS and GYC strains had 82% relationship index and between AFP-AGY and TS-GYC had 50% relationship index.

KERAGAMAN GENETIK GUPPY (Poecilia reticulata) MENGGUNAKAN METODE RAPD (Random Amplified Polymorphic DNA)

“…Guppy merupakan salah satu ikan yang digunakan sebagai model untuk mempelajari genetika populasi, inbreeding, dan heterosis (Mohammadabadi et al, 2021). Penelitian terkait variasi genetik pada beberapa strain ikan Guppy, diantaranya polymorfisme pada guppy jantan (Blows et al, 2003); variasi genetik gen pengontrol pigmen melanin (Tezuka et al, 2011); serta hubungan genetik empat strain Guppy yaitu Albino Full Platinum (AFP), Albino German Yellow (AGY), Top Sword (TS) dan Guppy Yellow Cobra (GYC) menggunakan metode RAPD-PCR (Nuradha et al, 2021).…”

“…Pengetahuan terkait variasi genetik pada ikan Guppy masih sangat diperlukan untuk menghindari terjadinya persilangan antara individu yang memiliki hubungan kekerabatan Jurnal Perikanan, 13 (4), 1032-1042 (2023) http://doi.org/10.29303/jp.v13i3.663 1034 sangat dekat (inbreeding) (Nuradha et al, 2021). Inbreeding dapat menimbulkan konsekuensi negatif seperti menurunnya keragaman genetik (Mohammadabadi et al, 2021).…”

“…Ukuran pita DNA pada primer OPA-7 berkisar 700 -1300 bp dan primer OPA-2 berkisar 450 -1500 bp. Hasil penelitian ini berbeda dengan hasil amplifikasi pita DNA Guppy strain Albino Full Platinum (AFP), Albino German Yellow (AGY), Top Sword (TS), dan Guppy Yellow Cobra (GYC) pada penelitian (Nuradha et al, 2021) menggunakan primer OPA-3 menghasilkan ukuran berkisar 216,70 -4850,45 bp.…”

“…Pita monomorfik adalah pita DNA yang ditemukan muncul di semua sampel strain yang diamati. Sedangkan pita DNA yang hanya ditemukan muncul di beberapa sampel yang diamati (tidak muncul di semua sampel) disebut pita polimorfik (Nuradha et al, 2021;Yustiati et al, 2021). Primer OPA-7 menghasilkan 3 pita monomorfik dan 2 pita polimorfik.…”

DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)

“…Random amplified polymorphic DNA (RAPD) is the simplest and most popular PCR (polymerase chain reaction) based technique which is easy to use, fast and low cost. RAPD has been used to find out the genetic variation in genus Synodontis (Abu-Almaaty and Ebied 2018), genus Scarus (Mohammed 2018), Coptodon zillii and Oreochromis aureus (Al-Khafaji et al 2019), Amblyceps mangois (Mukhopadhyay and Bhattacharjee 2019), Carangidae family (Al-Faisal et al 2019), Monopterus cuchia (Abedin et al 2020), Clarias batrachus (Miah et al 2020), guppy fish (Nuradha et al 2021), and Clarias gariepinus (Adesola et al 2020, Normala 2021). The study on genetic diversity of Channa punctata (Bloch 1793) was carried out by some workers such as Nagarajan et al (2006), Masih and Masih (2010), Bhat et al (2014), Kashyap et al (2016) but still information on comparative aspect of DNA polymorphism in different populations of the C. punctata is scanty.…”