Genetic relationships and evolution in Cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms

Gong, Li; Paris, Harry S.; Nee, Michael; Stift, Gertraud; Pachner, Martin; Vollmann, J.; Lelley, T.

doi:10.1007/s00122-011-1752-z

Cited by 78 publications

(70 citation statements)

References 49 publications

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“…These accessions included the most ancient and rustic morphotype of the subsp. pepo, the Pumpkin morphotype (Ferriol et al, 2003;Paris et al, 2003;Ferriol et al, 2007), but also landraces belonging to the vegetable marrow morphotype, developed in Europe after European contact with America and still appreciated in Mediterranean countries (Paris & Brown, 2005), and the more modern cocozelle and zucchini morphotypes, developed in Italy in the last century (Gong et al, 2012). Despite the diversity of the collection, no total or partial resistance or useful tolerance were identified in this subspecies.…”

Section: Discussionmentioning

confidence: 95%

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Resistance to Tomato leaf curl New Delhi virus in Cucurbita spp.

Sáez

Martínez

Ferriol

et al. 2016

Annals of Applied Biology

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Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus (family Geminiviridae) first reported in India and its neighbouring countries. ToLCNDV severely affects zucchini crop (Cucurbita pepo) in the main production areas of Southern Spain since 2012. This emerging begomovirus is a serious threat to this and other cucurbit crops. Breeding resistant cultivars is the most promising method for disease control, but requires the identification of sources of resistance in the Cucurbita genus. In this work, we screened for ToLCNDV resistance a large collection of Cucurbita spp. accessions, including landraces and commercial cultivars of the main cultivated species, C. pepo, Cucurbita moschata and Cucurbita maxima and wild species. The screening was performed using mechanical and whitefly inoculation. The level of resistance was assessed by scoring symptom severity and by measuring the virus content with quantitative polymerase chain reaction in selected genotypes. Diversity in the response was observed within and among species. Severe symptoms and high viral amounts were found at 30 days after mechanical and whitefly inoculation in C. pepo, in all accessions belonging to the Zucchini morphotype and to other morphotypes of both subspecies, pepo and ovifera, and even in the wild relative Cucurbita fraterna. C. maxima was also highly susceptible. This species showed characteristic symptoms of leaf decay and intense yellowing, different from those of mosaic, curling and internode shortening found in C. pepo. The only species showing resistance was C. moschata. Four accessions were symptomless or had some plants with only mild symptoms after three independent rounds of mechanical inoculation with different inoculum sources. Two of them also remained symptomless after virus inoculation with viruliferous whiteflies. ToLCNDV was detected in these asymptomatic accessions at 15 and 30 days post inoculation, but viral amounts were much lower than those found in susceptible genotypes, suggesting a high level of resistance. The symptoms in the susceptible accessions of this species were also different, with a characteristic leaf mottling, evolving to a severe mosaic. The newly identified C. moschata resistant accessions are good candidates for breeding programmes to avoid the damage caused by ToLCNDV.

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Section: Discussionmentioning

confidence: 95%

“…The knowledge of the genetic diversity of the species (Formisano et al, 2012;Gong et al, 2012;Esteras et al, 2013) allowed us to select a set of accessions representing most of the main morphotypes of the two subspecies of C. pepo. These accessions included the most ancient and rustic morphotype of the subsp.…”

Section: Discussionmentioning

confidence: 99%

Resistance to Tomato leaf curl New Delhi virus in Cucurbita spp.

Sáez

Martínez

Ferriol

et al. 2016

Annals of Applied Biology

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“…Genomic resources present in economically important species might be exploited in marginal crops with lower molecular resources. For example, successful SSRs transferability between species within a genus has been reported in Prunus (Wuensch and Hormaza, 2002), Cucumis (Danin-Poleg et al, 2001), Apiacea (Cavagnaro et al, 2011) and Cucurbita (Gong et al, 2012). The availability of SSR markers among the related species has been evaluated in many laboratories.…”

Section: Introductionmentioning

confidence: 99%

“…They are abundant in the genome and specific to species and genera, or to a lesser extent, can be preserved among plant family (Katzir et al, 1996;Gong et al, 2012). Genomic resources present in economically important species might be exploited in marginal crops with lower molecular resources.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Characterization of Turkish Landraces of <i>Cucumis melo</i> L.</span>

Yıldız

Akgul

Şensoy

2014

Not Bot Hort Agrobot Cluj

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Twenty-four accessions covering different groups of Cucumis melo L. from Eastern and South-eastern Anatolian regions of Turkey were characterized by using 43 morphological traits and 207 markers obtained from 31 ISSR and 16 SSR primers. The genetic relatedness was studied by examining the Euclidian/UPGMA dendrogram obtained from the combined phenotypicmolecular data. In the combined morphological-molecular dendrogram, there were two main clusters. Sweet and non-sweet melon groups were separated and the flexuosus group accessions were discriminated from the sweet ones, but the momordica group accession was clustered with the sweet ones. Unclear South-eastern Anatolian accessions were sub-clustered separately among the sweet ones. Principle component analysis (PCA) of morphological characters was used in detail to discriminate melon accessions. The cumulative proportion of variation reached 44% by first three PCA axes. The first component was mainly based on sex expression, ovary index, ovary shape, flesh thickness, seed cavity length, seed cavity width, soluble solids content, fruit shape, aroma, netting, and taste. The PCA plot based on all measured traits allowed distinction between flexuosus group, subsp. agrestis and reticulatus group. A high variation among groups was observed for the fruits characters. Netting, aroma and abscission of peduncle represent reticulatus group; a small fruit size, strong typical aroma and secondary colour distribution characterize dudaim group. Monoecy, very long fruit shape and mature fruit rind colour discriminate flexuous group; Ovary index, fruit size and flesh width distinguish subsp. agrestis group. These findings indicated wide range of variations for investigated characteristics in Turkish gene pool that provides a good source of diversity to use in melon improvement program for better yield and other traits of interest.

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“…Para el frijol representó la pérdida de su capacidad de dispersión y adecuación natural a consecuencia de la fijación de mutaciones en los genes que determinan la dehiscencia de sus vainas, la latencia en sus semillas, la insensibilidad al fotoperiodo, un hábito de crecimiento determinado y el incremento del tamaño y peso de la semilla (Koinange, Singh, & Gepts, 1996). En la calabaza, significó el decremento de su capacidad de dispersión y adecuación al ambiente natural a través de la fijación de mutaciones ligadas frutos y semillas más grandes, y frutos con carne menos fibrosa y no amarga ( Smith, 2006 ;Gong et al, 2012).…”

Section: (Figura 2)unclassified

La milpa del occidente de Mesoamérica: profundidad histórica, dinámica evolutiva y rutas de dispersión a Suramérica

Zizumbo‐Villarreal

Colunga‐GarcíaMarín

2017

rga

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ResumenEl núcleo del sistema alimentario precerámico en el occidente de Mesoamérica se pudo integrar con las poblaciones silvestres ancestrales del maíz, el frijol y la calabaza. El manejo agrícola incipiente de estas especies, año tras año, en un mismo sitio pudo derivar en la estructuración del sistema agroalimentario conocido como "milpa". En este trabajo intentamos responder: 1. ¿Cómo y cuándo se pudo integrar el sistema agroalimentario de milpa?; 2. ¿Qué grupos culturales humanos lo pudieron conformar?; 3. ¿Pudo ser posible la domesticación de las poblaciones silvestres del maíz, las calabazas y los frijoles en varios sitios de América?, 4. ¿A través de qué rutas y cuándo se pudieron difundir estos cultivos? Integramos evidencias paleo-ecológicas arqueológicas, etnobotánicas y genético-moleculares; analizamos el significado de la domesticación y la importancia de estudiar la dinámica evolutiva de los complejos poblacionales silvestre-domesticado de las especies cultivadas en la milpa y puntualizamos los retos, amenazas y oportunidades para sus recursos genéticos. El núcleo del sistema agroalimentario Milpa se estructuró con especies de la selva baja caducifolia hace cerca de 9,000 años. El maíz pudo arribar a Panamá, Ecuador y Perú hace 7,400-6,500 años. Las poblaciones silvestres ancestrales de las calabazas y los frijoles pudieron ser domesticadas tanto en Mesoamérica como en Suramérica. Para las plantas, la domesticación significó su dependencia de los humanos, y para los humanos un alto costo social no valorado actualmente. El estudio de la dinámica evolutiva de los complejos poblacionales de la milpa nos ayuda a desarrollar estrategias de conservación y restauración, y a establecer los riesgos de escape de genes domésticos y transgenes. Los recursos fitogenéticos de la milpa enfrentan grandes amenazas a raíz de los cambios sociales y económicos provocados por la globalización de los mercados. Sin embargo, constituyen una herramienta fundamental para enfrentar el cambio climático.Palabras clave: Mesoamérica, sistema agro-alimentario, Cucurbita, Phaseolus, Zea. The Milpa system of western Mesoamerica: historical depth, evolutionary dynamics and dispersal routes to South Americao AbstractThe core of the pre-ceramic food system in western Mesoamerica could have been structured with the ancestral wild populations of maize, beans and squash; in turn, incipient agricultural management of these species, year after year, at the same site, could have given rise to the Milpa agri-food system. This paper attempts to answer the following questions: 1. How and when did the Milpa agri-food system originate?, 2. Which human groups could have done it?, 3 Could the domestication of the ancestral wild populations of maize, squash and beans have been possible at several sites in the Americas?, 4. What were the dispersal routes? To answer these questions, paleoecological, archaeological, ethno-botanical, and molecular genetic evidence was collated. The significance of domestication and the importance of studying the evolut...

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Genetic relationships and evolution in Cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms

Cited by 78 publications

References 49 publications

Resistance to Tomato leaf curl New Delhi virus in Cucurbita spp.

Resistance to Tomato leaf curl New Delhi virus in Cucurbita spp.

Morphological and Molecular Characterization of Turkish Landraces of <i>Cucumis melo</i> L.</span>

La milpa del occidente de Mesoamérica: profundidad histórica, dinámica evolutiva y rutas de dispersión a Suramérica

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