Genetic relationships and population structure in three Italian Merino-derived sheep breeds

Lasagna, Emiliano; Bianchi, Matteo; Ceccobelli, Simone; Landi, Vincenzo; Martínez, Amparo Martínez; Vega-Pla, J.L.; Panella, Francesco; Bermejo, Juan Vicente Delgado; Sarti, Francesca Maria

doi:10.1016/j.smallrumres.2010.11.014

Cited by 28 publications

(32 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The D ST value obtained from this study can be considered as an indicator of low genetic diversity between breeds. In this study, the obtained MNA value in terms of the breeds was found to be higher than in the literature (20,26,27). MNA was an indicator of within-breed diversity.…”

Section: Discussioncontrasting

confidence: 75%

Genetic relationships among four Turkish sheep breeds using microsatellites

Yılmaz¹,

Sezenler²,

Sevim³

et al. 2015

Turk J Vet Anim Sci

View full text Add to dashboard Cite

IntroductionTurkey's proximity to domestication centers, along with its climate, topographical pattern, and agricultural structure that is formed according to plant cover, has created an appropriate environment for sheep breeding. Therefore, there are many sheep breeds in various regions and territories (1,2). Although there are many varieties and crossbred populations of sheep breeds in Turkey, 20 sheep breeds have been officially registered by the Ministry of Food, Agriculture, and Livestock (3). Even though Gökçeada (GA), Kıvırcık (KIV), Karacabey Merino (KM), and Sakız (SZ) sheep breeds, which are raised in the western Anatolian and Marmara regions, have a low percentage of native sheep population, each breed has certain significant characteristics. SZ sheep, known for their high fertility and milk production, and GA sheep, known for their milk production and high lamb survival rate, are raised along the coastline of the Aegean region. KIV and KM sheep breeds are known for their meat quality, wool, and meat production and are raised in western Turkey. For the purpose of improving the meat quality and fertility of the sheep population in western Turkey, the SZ and KIV breeds are commonly used in crossbreeding (4).Molecular genetic methods for identifying genetic structure and diversity in farm animals have shown a rapid development in recent years and have become widely used. Various molecular genetic methods have been developed for this purpose. Microsatellites that are specific to DNA regions are more widely used than other methods (5).This study aims to determine the genetic structure of GA and SZ sheep breeds, which are bred on the coastline of the Aegean region, and that of the KIV and KM sheep breeds, which are commonly bred in the Aegean and Marmara regions, by means of microsatellites. The information obtained from this study is based on microsatellite markers and will clarify the genetic relationships between these sheep breeds. It will also be helpful for determining current and future breeding programs and breed management and conservation strategies. Materials and methods Animal resources and DNA isolationThe animal material of the study consisted of a total of 250 animals belonging to GA, KIV, KM, and SZ sheep populations bred in the Aegean and Marmara regions. The origins and sample sizes of these 4 breeds are presented in Table 1. A DNA isolation kit (Applied Biological Materials Inc., Canada) was used for DNA extraction from blood samples.

show abstract

Section: Discussioncontrasting

confidence: 75%

Genetic relationships among four Turkish sheep breeds using microsatellites

Yılmaz¹,

Sezenler²,

Sevim³

et al. 2015

Turk J Vet Anim Sci

View full text Add to dashboard Cite

show abstract

“…Considering the animals genotyped, the population parameters related to average number of alleles (9.25), H O (0.63), H E (0.72) and AR (6.95) were considerably higher when compared with results from the other populations in study. The population parameters observed in the present study were also higher than those observed in other sheep breeds, both locally adapted, and exotic, from Spain (Calvo et al, 2011), India (Arora et al, 2011), Italy (Lasagna et al, 2011), and Cuba (Álvarez et al, 2012). Tolone et al (2012) found very similar results when analyzing five Sicilian sheep breeds with 20 microsatellites (average of 10.95 alleles per locus for the native sheep Pinzirita).…”

Section: Discussioncontrasting

confidence: 44%

Genetic diversity of locally adapted sheep from Pantanal region of Mato Grosso do Sul

Crispim¹,

Grisolia²,

Seno³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Sheep of the Pantaneiro breed and seven other breeds, raised in the State of Mato Grosso do Sul, Brazil, were genotyped using eight microsatellite loci. The aim of the present study was to determine the genetic variability, phylogenetic relationship, and patterns of gene introgression and miscegenation among the animals surveyed, to obtain information about the genetic structure of locally adapted sheep in Mato Grosso do Sul. A total of 195 animals were used for genetic analysis. The Pantaneiro breed had the largest average number of alleles/locus (9.25), and higher allelic richness (6.95), while the Dorper population had the lowest values for these parameters (4.88 and 3.86, respectively). Analysis of genetic distance values and genetic structure between populations made it possible to characterize these animals with regard to distinct genetic groups. Average expected heterozygosity ranged from 0.72 (Pantaneiro) to 0.55 (Dorper), while average observed heterozygosity ranged from 0.63 (White Dorper) to 0.54 (Dorper). On the basis of the statistical parameters evaluated, it 5459 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 5458-5466 (2013) Genetic diversity in Pantaneiro sheep was possible to demonstrate that when compared to other populations, the Pantaneiro breed represented a reservoir of genetic diversity with rare and useful alleles for genetic improvement, emphasizing the importance of preserving the breed.

show abstract

“…The number of the effective alleles was in general much lower (1.19 ÷ 7.83, mean value 4.60), for the very low frequency of many alleles. Fourteen loci had PIC values exceeding 0.5 (0.59  0.86), which was indicated as the limit for considering a marker highly informative (Botstein et al, 1980), while ETH10 had a value of 0.17, so poorly contributing to the characterization of the within-breed variation, as shown for other sheep breeds also (Lasagna et al, 2011). Moreover, the FST value for ETH10 locus (Table 1) was also low, indicating a limited variability of this locus across-breed.…”

Section: Marker Statisticsmentioning

confidence: 99%

Lamb meat traceability: The case of Sambucana sheep

Stasio

Piatti²,

Fontanella³

et al. 2017

Small Ruminant Research

Self Cite

View full text Add to dashboard Cite

Genetic traceability has a key role in the product certification, but it is rarely implemented in sheep so far, especially in the fresh meat sector. In this study, the case of the Sambucana sheep is analysed with the aim of developing a genetic system able to certify the origin of its traditional product, the Sambucano lamb, protected by a registered trademark. A set of 14 microsatellite markers was identified as an efficient tool to genetically discriminate the Sambucana sheep from other breeds potentially involved in mislabelling and to allow for an effective allocation test of meat cuts labelled as 'Guaranteed Sambucano lamb'. The paternity test proved to be an additional means to improve the reliability of the control. The traceability system here described is easy to implement in local minor sheep breeds and is recommended in the framework of meat certification.

show abstract

Genetic relationships and population structure in three Italian Merino-derived sheep breeds

Cited by 28 publications

References 36 publications

Genetic relationships among four Turkish sheep breeds using microsatellites

Genetic relationships among four Turkish sheep breeds using microsatellites

Genetic diversity of locally adapted sheep from Pantanal region of Mato Grosso do Sul

Lamb meat traceability: The case of Sambucana sheep

Contact Info

Product

Resources

About