Genetic relationships of 34 grapevine varieties and construction of molecular fingerprints by SSR markers

Abstract

In order to protect and promote the effective differentiation and rational utilization of grape germplasm resources, 34 grapevine varieties were selected from the National Germplasm Resources, Taigu Grape Nursery in China: 12 wine grapes (Group I), 14 table grapes (Group II) and 12 seedless grapes (Group III), 4 of which were in Group II, too. For the purpose of genetic characterization, 15 pairs of SSR (simple sequence repeat) primers were used. Sixty-six alleles were generated among Group I, including 64 polymorphic bands, with 1-8 alleles per locus. In Group II, a total of 54 alleles, from 1 to 8 (3.6 on average) alleles per locus, were identified. In Group III, 54 alleles with an average of 3.6 alleles per locus were identified and the percentage of polymorphic alleles was 94%. The 34 varieties clustered into two major clades in the dendrogram: V. vinifera with hybrid of V. vinifera and V. labrusca or hybrid of V. amurensisi and V. vinifera were clearly differentiated. After processing and filtering the raw data, we produced the molecular fingerprint code of each variety. These results showed that the SSR markers are useful for discrimination and analysis of genetic diversity of grapevine varieties. The SSR markers could be used to examine and distinguish the genetic resources among closely related varieties. This is also an effective tool for construction of a grapevine molecular fingerprinting system. The obtained data will be useful in grape breeding in the future.

Construction of a High-Density Genetic Map and Mapping of Firmness in Grapes (Vitis vinifera L.) Based on Whole-Genome Resequencing

“…The vineyard soil pH is 7.8. The average annual temperature in this region is 10.6 • C, annual sunshine duration is 2300 h, annual rainfall is 400-600 mm, frost-free period is 160-180 days, and effective accumulative temperature is 3675 • C [104]. The vines were planted in north-south orientation, with 0.8 m between individuals in a row and 2.5 m between rows.…”

Ecology and Genetics of Natural Populations of North American Vitis Species Used as Rootstocks in European Grapevine Breeding Programs

“…No M. rotundifolia-based SSR or EST-SSR markers were developed for this genus however other more suitable primer sets are available (Riaz et al, 2008). The development of other primers (Picq et al, 2014;Dong et al, 2018;etc. ) linked to aspects of resistance, phenology or environmental adaptation may also be of interest for further population genetic analysis of local Vitis.…”

Identification of Red Grapevine Cultivars (Vitis vinifera L.) Preserved in Ancient Vineyards in Axarquia (Andalusia, Spain)

“…The comparison of the SSR obtained was performed using a microsatellite toolkit v. 9.0 software [ 43 ]. Lastly, the microsatellite genotypes obtained after the analysis were compared to the genetic profiles given by Jiménez-Cantizano et al [ 30 ] and Lacombe et al [ 31 ], and to the data contained in European grapevine database of microsatellite profiles VIVC [ 21 ].…”

“…Nuclear microsatellite markers or simple sequence repeat (SSR) have been widely used to identify and genotype grapevine cultivars [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. In addition, the combination of genetic (microsatellite markers) and ampelographic methods allows the correct identification of cultivars [ 23 ].…”