Genetic Relationships of Heirloom Turnip (&lt;i&gt;Brassica rapa&lt;/i&gt;) Cultivars in Shiga Prefecture and Other Regions of Japan

Kubo, Nakao; Ueoka, Hayato; Satoh, Shigeru

doi:10.2503/hortj.utd-071

Cited by 10 publications

(7 citation statements)

References 29 publications

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“…Genomic DNA was isolated as previously reported ( Kubo et al 2019 ) from a single representative individual for each line. The genomic DNA of Horse mint-PI557757 used for the genome sequencing ( Vining et al 2017 ) was kindly supplied by Dr. K. Vining (Oregon State University, Corvallis, Oregon).…”

Section: Methodsmentioning

confidence: 99%

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Classification of Southeast Asian mints (Mentha spp.) based on simple sequence repeat markers

et al. 2022

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Mentha is a complex genus encompassing many species as a consequence of their interspecific hybridization and polyploidy. Southeast Asian mints have been poorly distinguished though they are widely used for culinary and medical purposes. In this study, we have analyzed Southeast Asian mints and known varieties as well as a related Lamiaceae species (Nepeta sp.) using simple sequence repeat (SSR) markers and leaf morphology. Two types of mints were clearly distinguished based on their venation pattern and leaf shape index. We developed 12 SSR markers that allowed good amplification in the Mentha and another Lamiaceae species. In the SSR-based phylogram, the Mentha lines could be delimited into groups I-VI. The Southeast Asian mints divided into groups I and II, and the phylogram separated most of the available species, with groups I and II containing the known species M. × cordifolia and M. arvensis, respectively. The separation of the two groups was supported by a population structure analysis. The SSR markers developed in this study enabled the simultaneous classification of mints and will help improve our understanding of the genetic composition of known mint varieties and as yet unclassified Southeast Asian mints.

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Section: Methodsmentioning

confidence: 99%

“…SSR fragments were amplified by polymerase chain reaction with fluorescently-labeled primers as reported previously ( Kubo et al 2019 ). The SSR alleles were recorded as binary data (presence/absence of band = 1/0) because more than two alleles were detected for each line in most cases.…”

Section: Methodsmentioning

confidence: 99%

Classification of Southeast Asian mints (Mentha spp.) based on simple sequence repeat markers

et al. 2022

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“…Up to 20 individuals per line from the 25 lines (total, 434 individuals) were investigated using SSR analysis. Genomic DNA was extracted from fresh leaf or root as reported previously (Kubo et al 2019b). Nine carrot SSR markers (Cavagnaro et al 2011) were used in the analysis (Supplemental Table 2).…”

Section: Dna Extraction Genotyping and Data Analysismentioning

confidence: 99%

Classification of “Kintoki ninjin” and other groups of carrot (Daucus carota) based on simple sequence repeat markers

et al. 2019

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Carrot (Daucus carota) is cultivated in temperate regions for its taproot. Eastern and Western types have been differentiated. In Japan, the former type is categorized into Kintoki, Takinogawa oonaga, and Toso, with a few local cultivars. However, their genetic relationships are unclear because of the paucity of reports. We classified the Japanese Eastern and selected Western types based on simple sequence repeat (SSR) markers. Field traits, including root weight, length, diameter, and skin color, were also examined. Our field tests showed clear differences between the Kintoki and Western-type cultivars, confirming their differentiation. A phylogram based on nine SSRs classified 24 cultivars into groups I and II. Group I included all Eastern-type carrots examined (Kintoki and Toso groups, plus two local and two foreign cultivars), with the exception of an Indian cultivar ('Pusa rudhira red'). Among them, red carrots including Kintoki were clustered into two subgroups. Westerntype, Eastern-Western hybrid, and 'Pusa rudhira red' were included in group II. A population structure analysis revealed the split between the Eastern and the other types. This study elucidates the genetic characteristics of the Eastern type of carrot, which will be valuable information for carrot breeding, especially when using the Eastern type as a source.

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“…Its plant parts, including both roots and leaves, are important for human consumption and also for animal feed (Bonnema et al., 2019 ; Chung et al., 2016 ; Liu et al., 2019 ; Lou et al., 2017 ). The main edible organ is consisted of the swollen hypocotyl and root (Kubo et al., 2019 ). It is generally grown as a field vegetable or home garden crop (Abbasi et al., 2011 ).…”

Section: Introductionmentioning

confidence: 99%

“…is consisted of the swollen hypocotyl and root (Kubo et al, 2019). It is generally grown as a field vegetable or home garden crop (Abbasi et al, 2011).…”

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Selection of superior accessions of turnip (Brassica rapa var. rapa L.) based on tuber quality‐related characters

Khadivi

Mirheidari

Moradi

2022

Food Science & Nutrition

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Turnip ( Brassica rapa var. rapa L.) (syn. B. campestris L. ssp. rapifera Sinsk) is an important crop species belonging to the Brassicaceae family. The 185 accessions belonging to this crop were collected from several areas of Toodshak region in Isfahan province, Iran, and their tubers were cultivated under homogeneous conditions in loamy clay soil. The morphological traits of different organs of those accessions were evaluated. Significant variations were detected among the accessions studied based on the traits recorded. Tuber shape showed high variation and included globose, oblong, ovate, obovate, and fusiform. Also, tuber skin color was highly variable, including white, bicolor white–violet, light violet, and dark violet. Tuber weight ranged from 1.56 to 35.70 g, while total soluble solids (TSS) of tuber flesh ranged from 7.00 to 11.80%. Principal component analysis (PCA) showed that 18 components were extracted by explaining 74.88% of total variance. The dendrogram obtained based on all the characters measured clustered the accessions into two major clusters. Sixteen accessions were placed into the first cluster, while the remaining accessions were placed into the second cluster which was divided into six subclusters. High level of morphological variabilities was observed among the accessions, which is applicable and useful for B. rapa var. rapa breeding programs. Based on the commercial and quality traits, 17 accessions could be selected for direct cultivation. Also, the promising accessions identified here can be utilized directly in breeding programs for genetic enhancement of this crop.

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Genetic Relationships of Heirloom Turnip (<i>Brassica rapa</i>) Cultivars in Shiga Prefecture and Other Regions of Japan

Cited by 10 publications

References 29 publications

Classification of Southeast Asian mints (<i>Mentha</i> spp.) based on simple sequence repeat markers

Classification of Southeast Asian mints (<i>Mentha</i> spp.) based on simple sequence repeat markers

Classification of “Kintoki ninjin” and other groups of carrot (<i>Daucus carota</i>) based on simple sequence repeat markers

Selection of superior accessions of turnip (Brassica rapa var. rapa L.) based on tuber quality‐related characters

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