Genetic relationships of the Japanese persimmon Diospyros kaki (Ebenaceae) and related species revealed by SSR analysis

Abstract

ABSTRACT. Simple sequence repeat (SSR) molecular markers based on 18 primers were employed to study the genetic relationship of Japanese persimmon (Diospyros kaki) specimens. Two hundred and sixty-two bands were detected in 30 Japanese persimmon samples, including 14 Japanese and 10 Chinese genotypes of Japanese persimmon (Diospyros kaki) and six related species, D. lotus, D. glaucifolia, D. oleifera, D. rhombifolia, D. virginiana, and Jinzaoshi (unclassified -previously indicated to be D. kaki). All SSR primers developed from D. kaki were successfully employed to reveal the polymorphism in other species of Diospyros. Most of the primers were highly polymorphic, with a degree of polymorphism equal to or higher than 0.66. The results from the neighbor-joining dendrogram and the principal coordinate analysis diagram were the same; i.e., the Chinese and Japanese genotypes and related species were separated and the relationships revealed were consistent with the known pedigrees. We also concluded that 'Xiangxitianshi' from Xiangxi municipality, Hunan Province, China, is actually a sport or somaclonal variant of 'Maekawa-Jirou', and that 'Jinzaoshi' should be classified as a distinct species of Diospyros. We found that SSR markers are a valuable tool for the estimation of genetic diversity and divergence in Diospyros.

Development of simple sequence repeat markers in persimmon (Diospyros L.) and their potential use in related species

“…Among the different molecular markers, SSRs, which are also called microsatellites, are the best-suited markers for genotyping the Diospyros genus (Luo et al, 2013). Guo and Luo (2006a, 2008, 2011 reported several SSR markers by ISSRsuppression PCR. Soriano et al (2006) developed 22 SSRs using a CT/AG-repeat-enriched library.…”

Genetic Diversity and Breeding of Persimmon

“…were subjected to various doses of gamma rays, 15 and 20 gray from a 60 Co source. In this study, Naval et al [55] found that the most favorable gamma irradiation dose combining survival and mutation induction was 20 gray.…”

“…Breeding and Health Benefits of Fruit and Nut Crops that the placement of several Japanese cultivars within the European cultivar group suggests that European cultivars were developed from Japanese germplasm relatively recent and differences among cultivars are much greater than differences among cultivar groups regarding AFLP markers. In addition, Guo and Luo [60] indicated that SSR markers are a valuable tool for the estimation of genetic diversity and divergence in Diospyros.…”

Anti-inflammatory, Antipyretic and Analgesic Activities of Persimmon (Diospyros kaki) Leaves in Animal Model

“…The genus Diospyros has more than 500 species and is the largest genus of the Ebenaceae family. Diospyros has remarkable economic value, particularly Diospyros kaki L. (Ebenaceae), which is conventionally used as food in multiple countries (Guo and Luo, 2011).…”