Genetic resources and features of clonal micropropagation of Far Eastern species of <i>Actinidia</i>

Molkanova, O. I.; Krakhmaleva, I. L.; Kozak, Natal'ya

doi:10.1051/bioconf/20224303021

Cited by 2 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The explants were rinsed for 5-7 min in 5-7% calcium hypochlorite solution, and 1-2 drops of Tween 20 were added. After adding each reagent, the explants were washed 4-5 times in sterile distilled water Krakhmaleva;Kozak, 2022).…”

Section: Surface Sterilizationmentioning

confidence: 99%

“…The initiation medium QL (Quoirin and Lepoivre, 1977), solidified with 6.6 g L -1 agar (Roeper, Germany), containing 30 g L -1 sucrose and 0.3 mg L -1 6-Benzylaminopurine (6-BAP) (Sigma, USA), was used for shoot induction Krakhmaleva;Kozak, 2022). The medium was adjusted to pH 5.8 with potassium hydroxide and sterilized in an autoclave (WAC-60; Daihan Scientific, South Korea) using pressurized saturated steam (P = 101 kPa) at 121 °C for 20 min.…”

Section: Surface Sterilizationmentioning

confidence: 99%

“…Some studies, however, have also investigated the micropropagation of other species, such as A. arguta (Hameg;Gallego;Barreal, 2017;Hameg et al, 2020), A. kolomikta (Kovac, 1993), A. polygama (Takahashi et al, 2004), and A. melanandra (Debenham;Seelye;Mullan, 2016). These researchers investigated the effect of macronutrients, plant growth regulators, and genetic characteristics on the in vitro shoot regeneration of these species (Hameg;Gallego;Barreal, 2017;Malaeva;Molkanova, 2021;Krakhmaleva;Kozak, 2022).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Plant growth regulators on the micropropagtion of Actinidia cultivars

Krakhmaleva,

Molkanova,

Orlova

et al. 2023

Ciênc. agrotec.

View full text Add to dashboard Cite

Actinidia Lindl., commonly known as kiwifruit, is a valuable berry crop. The area of commercial kiwifruit plantations is increasing; the global production of kiwifruit is about 0.62% of the total production of major fruit crops. The use of biotechnological methods, which can significantly accelerate the propagation of quality planting materials, is considered to be relevant for the propagation of this crop. In this study, we optimized the culture medium composition at the micropropagation stage for the effective cultivation of promising cultivars of A. arguta, A. kolomikta, and A. polygama. We investigated the features of Actinidia morphogenesis depending on the genotype, the concentration of 6-Benzylaminopurine (0.5, 0.8, and 1.0 mg L-1), and plant growth regulators (6-Benzylaminopurine, meta-topolin, and 2-isopentenyladenine at a concentration of 0.5 mg L-1) in the media Quoirin and Lepoivre. Actinidia arguta (multiplication rate of 8.0) and A. polygama (6.8) developed faster at the micropropagation stage compared to A. kolomikta (4.9). The studied Actinidia representatives were cultured most effectively on Quoirin and Lepoivre media supplemented with 0.5 mg L-1 meta-topolin, compared to the media containing 0.5 mg L-1 6-Benzylaminopurine and 0.5 mg L-1 2-isopentenyladenine. The use of meta-topolin in the medium contributed to the increase in various morphometric traits, such as the height of microshoots (up to 28% depending on the species), their number (up to 52%), and their multiplication rate (up to 42%). We also recorded a high morphogenic capacity of the investigated species.

show abstract

Section: Surface Sterilizationmentioning

confidence: 99%

Section: Surface Sterilizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Plant growth regulators on the micropropagtion of Actinidia cultivars

Krakhmaleva,

Molkanova,

Orlova

et al. 2023

Ciênc. agrotec.

View full text Add to dashboard Cite

show abstract

In Vitro Morpho-Anatomical and Regeneration Features of Cultivars of Actinidia kolomikta (Maxim.) Maxim

Krakhmaleva,

Molkanova,

Orlova

et al. 2024

Horticulturae

View full text Add to dashboard Cite

Actinidia kolomikta (Maxim) Maxim. is a winter-hardy species of the genus Actinidia Lindl., whose fruits are valued for their high content of vitamin C and other bioactive compounds. The use of biotechnological propagation methods significantly accelerates the production of quality planting materials for this crop. This study revealed the regeneration features of promising A. kolomikta cultivars. The main morphometric parameters of explants were determined in regard to the effect of different iron chelates (FeEDTA and FeEDDHA) and cytokinins (6-benzylaminopurine, meta-Topolin, and 2-izopentyladenine) in the Quoirin and Lepoivre medium. FeEDTA-supplemented media were optimal for explant culture. Meta-Topolin was found to promote the formation of adventitious microshoots at the base of explants and bud activation, which increased the multiplication rate by 1.5 and 1.7 times compared to the media with 6-benzylaminopurine and 2-izopentyladenine. The morpho-anatomical studies revealed the structural organization of assimilation tissues and the stomatal apparatus of A. kolomikta under different culture conditions (field, in vitro, and ex vitro). The stomata in vitro were round and had a larger area, lower thickness, and a lower layer number of mesophyll compared to field conditions. The transfer from in vitro to ex vitro caused gradual normalization of the leaf structure: a decrease in the stomatal number and area, changes in shape (from round to elliptical), and an increase in the mesophyll thickness.

show abstract

Genetic resources and features of clonal micropropagation of Far Eastern species of Actinidia

Cited by 2 publications

References 14 publications

Plant growth regulators on the micropropagtion of Actinidia cultivars

Plant growth regulators on the micropropagtion of Actinidia cultivars

In Vitro Morpho-Anatomical and Regeneration Features of Cultivars of Actinidia kolomikta (Maxim.) Maxim

Contact Info

Product

Resources

About