Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa

Abstract

Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.

Chara globata Mig. (Streptophyta: Charales): rare species revised

“…The DNA was extracted with the modified Echt's method (echt et al 1992;Kiselev et al 2011) from the specimens collected in the Maagan Michael Quarry (Israel) and oxbow lake of the Yeya River (Russia), where the sizes of specimens were allowed to detach from several apical parts of thalli without obvious deterioration. A partial rbcL gene was amplified with primers RH1 and1385R (manhaRt et al 1994), and PCR products were directly sequenced using the ABI Big Dye Terminator v.3.1 Cycle Sequencing kit according to the manufacturer's instructions and the ABI 3130 genetic analyzer at the Institute of Biology and Soil Science FEB RAS.…”

DETECTION OF MOLECULAR MARKERS ASSOCIATED WITH SALT TOLERANCE IN ALFALFA (Medicago sativa L.)

“…Also, Wenzel (1992) emphasized the potential for DNA markers-based diagnosis of abiotic stress tolerance in plants. Echt et al (1992) reported that RAPD markers appeared to be useful for the rapid development of genetic information in alfalfa. Also, Dias et al (2004) reported that the RAPD markers were efficient in separating all the accessions analyzed individually.…”

Panax ginsengnatural populations: their past, current state and perspectives1

“…Enzymes were extracted from fresh leaves or those frozen in liquid nitrogen. AFLP and SSR analysis Genomic DNA was extracted according to Echt et al [11] and then purified by Murray and Thompson [12] .…”