Genetic Similarity of<i>Campylobacter</i>Isolates in Humans, Food, and Water Sources in Central Poland

Rozynek, E; Antos-Bielska, Małgorzata; Dzierżanowska-Fangrat, Katarzyna; Szczepańska, Bernadeta; Trafny, Elżbieta Anna

doi:10.1089/fpd.2009.0407

Cited by 14 publications

(11 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…10 However, over the years, various studies have reported an increase in the resistance of Campylobacter to these drugs. 8,17,19,59,71,82,85 The rise of antimicrobial resistance among Campylobacter spp. has been linked to the use of antimicrobials in veterinary medicine and in farming practices, mainly as prophylactic agents and growth promoters 88,97 This is important, because some of the resistant isolates have been suspected to spread from food animals to humans.…”

mentioning

confidence: 99%

“…has been linked to the use of antimicrobials in veterinary medicine and in farming practices, mainly as prophylactic agents and growth promoters 88,97 This is important, because some of the resistant isolates have been suspected to spread from food animals to humans. 71,79 While antimicrobial-resistant Campylobacter spp. has been reported worldwide, the situation might be more severe in developing countries, where there is widespread and largely uncontrolled use of antimicrobials.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and Antimicrobial Resistance ofCampylobacterIsolated from Dressed Beef Carcasses and Raw Milk in Tanzania

Kashoma

Kassem

John

et al. 2016

Microbial Drug Resistance

View full text Add to dashboard Cite

Campylobacter species are commonly transmitted to humans through consumption of contaminated foods such as milk and meat. The aim of this study was to investigate the prevalence, antimicrobial resistance, and genetic determinants of resistance of Campylobacter isolated from raw milk and beef carcasses in Tanzania. The antimicrobial resistance genes tested included bla OXA-61 (ampicillin), aph-3-1 (aminoglycoside), tet(O) (tetracycline), and cmeB (multi-drug efflux pump). The prevalence of Campylobacter was 9.5% in beef carcasses and 13.4% in raw milk, respectively. Using multiplex-polymerase chain reaction (PCR), we identified 58.1% of the isolates as Campylobacter jejuni, 30.7% as Campylobacter coli, and 9.7% as other Campylobacter spp. One isolate (1.6%) was positive for both C. jejuni and C. coli specific PCR. Antimicrobial susceptibility testing using the disk diffusion assay and the broth microdilution method showed resistance to: ampicillin (63% and 94.1%), ciprofloxacin (9.3% and 11.8%), erythromycin (53.7% and 70.6%), gentamicin (0% and 15.7%), streptomycin (35.2% and 84.3%), and tetracycline (18.5% and 17.7%), respectively. Resistance to azithromycin (42.6%), nalidixic acid (64.8%), and chloramphenicol (13%) was determined using the disk diffusion assay only, while resistance to tylosin (90.2%) was quantified using the broth microdilution method. The bla OXA-61 (52.6% and 28.1%), cmeB (26.3% and 31.3%), tet(O) (26.3% and 31.3%), and aph-3-1 (5.3% and 3.0%) were detected in C. coli and C. jejuni. These findings highlight the extent of antimicrobial resistance in Campylobacter occurring in important foods in Tanzania. The potential risks to consumers emphasize the need for adequate control approaches, including the prudent use of antimicrobials to minimize the spread of antimicrobial-resistant Campylobacter.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Prevalence and Antimicrobial Resistance ofCampylobacterIsolated from Dressed Beef Carcasses and Raw Milk in Tanzania

Kashoma

Kassem

John

et al. 2016

Microbial Drug Resistance

View full text Add to dashboard Cite

show abstract

“…More recently, a multilocus sequence typing (MLST) scheme for C. jejuni (12) has emerged as the leading method for subtyping on the strength of several key features. These include the high portability of nucleotide sequence-based typing data, which has greatly facilitated interlaboratory comparisons and has allowed for the establishment of a global MLST database, which has contributed to a greater understanding of the population structure and global epidemiology of C. jejuni and related organisms (42).Numerous studies have shown the importance of molecular subtyping in tracing the molecular epidemiology of campylobacteriosis through the analysis of isolates from clinical, livestock, wildlife, and environmental sources (11,24,32,35,39,40,52,54,56). However, there are a number of inherent challenges to the conventional genotyping of campylobacter populations, which are characterized by high genetic diversity, weak clonality, and high levels of intraspecific recombination (13,66).…”

mentioning

confidence: 99%

Development and Validation of a Comparative Genomic Fingerprinting Method for High-Resolution Genotyping of Campylobacter jejuni

et al. 2012

View full text Add to dashboard Cite

e Campylobacter spp. are a leading cause of bacterial gastroenteritis worldwide. The need for molecular subtyping methods with enhanced discrimination in the context of surveillance-and outbreak-based epidemiologic investigations of Campylobacter spp. is critical to our understanding of sources and routes of transmission and the development of mitigation strategies to reduce the incidence of campylobacteriosis. We describe the development and validation of a rapid and high-resolution comparative genomic fingerprinting (CGF) method for C. jejuni. A total of 412 isolates from agricultural, environmental, retail, and human clinical sources obtained from the Canadian national integrated enteric pathogen surveillance program (C-EnterNet) were analyzed using a 40-gene assay (CGF40) and multilocus sequence typing (MLST). The significantly higher Simpson's index of diversity (ID) obtained with CGF40 (ID ‫؍‬ 0.994) suggests that it has a higher discriminatory power than MLST at both the level of clonal complex (ID ‫؍‬ 0.873) and sequence type (ID ‫؍‬ 0.935). High Wallace coefficients obtained when CGF40 was used as the primary typing method suggest that CGF and MLST are highly concordant, and we show that isolates with identical MLST profiles are comprised of isolates with distinct but highly similar CGF profiles. The high concordance with MLST coupled with the ability to discriminate between closely related isolates suggests that CFG40 is useful in differentiating highly prevalent sequence types, such as ST21 and ST45. CGF40 is a high-resolution comparative genomics-based method for C. jejuni subtyping with high discriminatory power that is also rapid, low cost, and easily deployable for routine epidemiologic surveillance and outbreak investigations. Campylobacter spp. are a leading cause of bacterial gastroenteritis worldwide (20), and most cases are thought to be the direct result of infection by C. jejuni or C. coli (20,43). Risk factors for campylobacteriosis include exposure to contaminated water, milk, and various food products, such as poultry (1,3,6,21). The development and implementation of effective control measures for these pathogens hinge on the identification of sources of infection. Although the ingestion of contaminated food or water and animal contact play a significant role in the epidemiology of campylobacteriosis, efforts to track sources of Campylobacter infection are hampered by the sporadic nature of campylobacteriosis (25), the infrequent association with outbreaks of disease, and widespread reservoirs that include water, livestock, domestic animals, and wildlife (8,17,46,65,67).A number of different molecular subtyping methods, such as pulsed-field gel electrophoresis (PFGE), restriction fragment length polymorphism analysis of the flagellin gene (flaA RFLP), and the DNA sequencing of the flagellin gene short variable region (flaA SVR), have been used to identify genotypic clusters of Campylobacter in the context of molecular epidemiology (18, 33). More recently, a multilocus sequence typing (ML...

show abstract

“…(Acke et al, 2010;Rozynek et al, 2010;Lazou et al, 2014). Our PFGE results showed i44.9 % genomic diversity amongst the strains (Fig.…”

Section: Discussionmentioning

confidence: 58%

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

Gomes

Souza

Passaglia

et al. 2016

Journal of Medical Microbiology

View full text Add to dashboard Cite

Campylobacter coli and Campylobacter jejuni are two of the most common causative agents of food-borne gastroenteritis in numerous countries worldwide. In Brazil, campylobacteriosis is underdiagnosed and under-reported, and few studies have molecularly characterized Campylobacter spp. in this country. The current study genotyped 63 C. coli strains isolated from humans (n512), animals (n521), food (n510) and the environment (n520) between 1995 and 2011 in Brazil. The strains were genotyped using pulsed-field gel electrophoresis (PFGE), sequencing the short variable region (SVR) of the flaA gene ( flaA-SVR) and high-resolution melting analysis (HRMA) of the clustered regularly interspaced short palindromic repeat (CRISPR) locus to better understand C. coli genotypic diversity and compare the suitability of these three methods for genotyping this species. Additionally, the discrimination index (DI) of each of these methods was assessed. Some C. coli strains isolated from clinical and non-clinical origins presented $80 % genotypic similarity by PFGE and flaA-SVR sequencing. HRMA of the CRISPR locus revealed only four different melting profiles. In total, 22 different flaA-SVR alleles were detected. Of these, seven alleles, comprising gt1647-gt1653, were classified as novel. The most frequent genotypes were gt30 and gt1647.This distribution reveals the diversity of selected Brazilian isolates in comparison with the alleles described in the PubMLST database. The DIs for PFGE, flaA-SVR sequencing and CRISPR-HRMA were 0.986, 0.916 and 0.550, respectively. PFGE and flaA-SVR sequencing were suitable for subtyping C. coli strains, in contrast to CRISPR-HRMA. The high genomic similarity amongst some C. coli strains confirms the hypothesis that environmental and food sources potentially lead to human and animal contamination in Brazil.

show abstract

Genetic Similarity ofCampylobacterIsolates in Humans, Food, and Water Sources in Central Poland

Cited by 14 publications

References 12 publications

Prevalence and Antimicrobial Resistance ofCampylobacterIsolated from Dressed Beef Carcasses and Raw Milk in Tanzania

Prevalence and Antimicrobial Resistance ofCampylobacterIsolated from Dressed Beef Carcasses and Raw Milk in Tanzania

Development and Validation of a Comparative Genomic Fingerprinting Method for High-Resolution Genotyping of Campylobacter jejuni

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

Contact Info

Product

Resources

About