Genetic similarity of iridoviruses from Japan and Thailand

Miyata, Masato; Matsuno, Koya; Jung, Sung‐Ju; Danayadol, Yaowanit; Miyazaki, Teruo

doi:10.1046/j.1365-2761.1997.d01-115.x

Cited by 53 publications

(37 citation statements)

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“…Each viral isolate was inoculated onto confluent monolayers of CRF-1 cells in three replicate 25-cm 2 flasks as described above. DNA extraction from these iridovirus isolates was performed by a proteinase K lysis method (30). Briefly, the infected cell culture including supernatant was centrifuged at 50 ϫ g for 20 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

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Phenotypic Diversity of Infectious Red Sea Bream Iridovirus Isolates from Cultured Fish in Japan

Shinmoto

Taniguchi

Ikawa

et al. 2009

Appl Environ Microbiol

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Megalocytivirus is causing economically serious mass mortality by infecting fish in and around the Pacific region of Asia. The recent emergence of many new iridoviruses has drawn attention to the marked taxonomic variation within this virus family. Most studies of these viruses have not included extensive study of these emergent species. We explored the emergence of red sea bream iridovirus (RSIV) on a fish farm in Japan, and we specifically endeavored to quantify genetic and phenotypic differences between RSIV isolates using in vitro and in vivo methods. The three isolates had identical major capsid protein sequences, and they were closely related to Korean RSIV isolates. In vitro studies revealed that the isolates differed in replication rate, which was determined by real-time quantitative PCR of viral genomes in infected cells and cell culture supernatant, and in cell viability, estimated by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay for infected cells. In vivo studies showed that the isolates exhibit different virulence characteristics: infected red sea bream showed either acute death or subacute death according to infection with different isolates. Significant differences were seen in the antigenicity of isolates by a formalin-inactivated vaccine test. These results revealed that variant characteristics exist in the same phylogenetic location in emergent iridoviruses. We suggest that this strain variation would expand the host range in iridoviral epidemics.

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Section: Methodsmentioning

confidence: 99%

“…The incidence of iridovirus infection has been increasing among cultured fish in Japan (29,30). In addition, genetic and phenotypic iridovirus variants suggest the presence of diverse variants in this virus group (16).…”

mentioning

confidence: 99%

Phenotypic Diversity of Infectious Red Sea Bream Iridovirus Isolates from Cultured Fish in Japan

Shinmoto

Taniguchi

Ikawa

et al. 2009

Appl Environ Microbiol

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“…DNA was extracted from ALIV and from DGIV by the Proteinase K lysis method (Sambrook et al 1989, modified by Miyata et al 1997). Approximately 10 to 50 mg of spleens excised from naturally iridovirus-infected dwarf gourami or pieces of the body of naturally iridovirus-infected lampeye was homogenized in a 0.5 ml aliquot of the 1× Proteinase K lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 2% (w/v) sodium dodecyl sulfate, 10 µg ml -1 Proteinase K) in a glass homogenizer.…”

Section: Methodsmentioning

confidence: 99%

Iridovirus disease in two ornamental tropical freshwater fishes: African lampeye and dwarf gourami

Sudthongkong¹,

Miyata²,

Miyazaki³

2002

Dis. Aquat. Org.

Self Cite

120

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Many species of ornamental freshwater fishes are imported into Japan from all over the world. We found African lampeye Aplocheilichthys normani and dwarf gourami Colisa lalia suffering from an iridovirus infection just after being imported by tropical fish wholesalers from Singapore. African lampeye were cultured on the Indonesian Island of Sumatra and dwarf gourami were cultured in Malaysia before export. Diseased fishes displayed distinct histopathological signs of iridovirus infection: systemic appearance of inclusion body-bearing cells, and necrosis of splenocytes and hematopoietic cells. Electron microscopy revealed viral particles (African lampeye:180 to 200 nm in edge to edge diameter; dwarf gourami: 140 to 150 nm in diameter) in an inclusion body within the cytoplasm of inclusion body-bearing cells as well as in the cytoplasm of necrotized cells. Experimental infection with an iridovirus isolate from African lampeye (ALIV) revealed pathogenicity of ALIV to African lampeye and pearl gourami Trichogaster leeri. Polymerase chain reaction (PCR) products from ALIV and an iridovirus isolate from dwarf gourami (DGIV) using iridovirus-specific primers were indistinguishable. The nucleotide sequence of PCR products derived from ALIV (696 base pairs) and DGIV (701 base pairs) had 95.3% identity. These results indicate that ALIV and DGIV have a single origin. KEY WORDS: Tropical iridovirus · African lampeye · Dwarf gourami · Inclusion body-bearing cells · Polymerase chain reaction assay · DNA sequence Resale or republication not permitted without written consent of the publisherDis Aquat Org 48: [163][164][165][166][167][168][169][170][171][172][173] 2002 brown-spotted groupers Epinephelus tauvina (Chua et al. 1994) and E. malabaricus (Danayadol et al. 1996), farmed in Singapore and Thailand, respectively; sea bass, Lateolabrax sp., captured in the South China Sea ; mandarin fish Siniperca chuatsi, reared in offshore pens in the South China Sea in China (He et al. 2000); red sea bream Chrisophylus major (= Pagrus major, Inouye et al. 1992), yellowtail Seriola quinqueradiata, and striped jack Caranx delicatissimus, etc., farmed in Japan (Miyazaki unpubl.); and striped beakperch Oplegnathus fasciatus, cultured in Korea (Jung & Oh 2000).Here we present the results of histopathological and electron microscopic (EM) examinations of the diseased African lampeye and dwarf gourami. We report the results of artificial infections with an isolate from African lampeye to African lampeye and pearl gourami. We also describe a polymerase chain reaction (PCR) assay and DNA sequence analysis of the PCR products for identification of iridovirus isolates from African lampeye (ALIV) and dwarf gourami (DGIV). MATERIALS AND METHODSHistopathological and EM examinations, virus culture in natural outbreaks. Diseased African lampeye and dwarf gourami were found just after being imported to Japan from Singapore by tropical fish wholesalers. The African lampeye had been cultured in freshwater ponds on Sumatra Island, Indonesia, a...

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“…RSIV has caused serious fish losses, which result in severe economic losses. The presence of the virus in fish has been reported from Asian regions including Korea, Japan, China, Taiwan and Thailand (Inoue et al, 1992;Chua et al, 1994;Kasornchandra and Khongpradit, 1997;Miyata et al, 1997;Jung and Oh, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…E-mail: tklee@kiost.ac. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License and caused huge economic losses (Inoue et al, 1992;Chua et al, 1994;Kasornchandra and Khongpradit, 1997;Miyata et al, 1997;Jung and Oh, 2000). The RSIV was first isolated from the cultured red sea bream Pagrus major in 1992 (Imajoh et al, 2007;Inoue et al, 1992;Caipang et al, 2006), and has been found to infect more than 30 species of cultured fishes since then.…”

Section: Introductionmentioning

confidence: 98%

Rapid and sensitive detection of iridovirus by loop-mediated isothermal amplification (LAMP)

Hwang¹,

Suh²,

Park³

et al. 2015

Afr. J. Microbiol. Res.

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Red seabream iridovirus (RSIV), a member of the Iridoviridae family, is the causative pathogen of some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of RSIV infection. In the present study, a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the sensitive and rapid detection of RSIV infection in fishes was developed. Using a set of synthesized primers matching a specific region of the RSIV genome (GenBank accession no.: AB666336.1), the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficiently detect RSIV infection in red sea bream. Our results provide a simple and convenient method for the detection of viral infection in aquatic organisms.

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Genetic similarity of iridoviruses from Japan and Thailand

Cited by 53 publications

References 0 publications

Phenotypic Diversity of Infectious Red Sea Bream Iridovirus Isolates from Cultured Fish in Japan

Phenotypic Diversity of Infectious Red Sea Bream Iridovirus Isolates from Cultured Fish in Japan

Iridovirus disease in two ornamental tropical freshwater fishes: African lampeye and dwarf gourami

Rapid and sensitive detection of iridovirus by loop-mediated isothermal amplification (LAMP)

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