Genetic stability analysis of tissue culture derived date palm cv. Dedhi plants using IRAP markers

Mirani, Abdul Aziz; Jatoi, Mushtaque Ahmed; Bux, Lal; Teo, Chee How; Kabiita, Arinaitwe Ivan; Harikrishna, Jennifer Ann; Markhand, Ghulam Sarwar; Jatt, Tahira; Solangi, Najamuddin; Abro, Sabra; Channa, Ghulam Sarwar

doi:10.1016/j.chnaes.2021.02.011

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…They also proposed the early identi cation and diagnosis of off-type plants to prevent the incidence and proliferation of these abnormalities. Mirani et al (2022) also observed no signi cant difference in the genetic diversity of off-shoot and tissue culture date palm cv. Dedhi.…”

Section: Molecular Studiesmentioning

confidence: 80%

“…Studies conducted by genetic markers showed differences between off-shoot and tissue culture date palms. This dissimilarity can be due to gene expression changes (Shair et al, 2016;Mirani et al, 2022). The date palm-speci c inter-retrotransposon ampli ed polymorphism (IRAP) and miniature inverted-repeat transposable element (MITE) markers were applied to identify the somaclonal genetical changes of date palm plants obtained from tissue culturing.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Pollen Treatments on Fruit Yield and Gene Expression in Offshoot and Tissue Culture Date Palms (Phoenix dactylifera L. cv. Barhi)

Alavipour,

Moallemi,

Khaleghi

et al. 2023

Preprint

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The low performance of date palm cv. Barhi resulting from tissue culturing is one of the main challenges in the production of these plants. On the other hand, the final yield of the plant depends on various metabolic and biochemical factors which are caused by gene expression. The plant reacts to environmental factors to survive in different growth and environmental conditions through gene expression. This experiment was conducted to investigate the relationship between the expression of certain genes before and up to two weeks after pollination with the yield of off-shoot and tissue culture of Phoenix dactylifera L. cv. Barhi (10-year-old). Off-shoot and tissue-culture date palms were pollinated with Red Ghanami pollen, Green Ghanami pollen, and Green Ghanami + Red Ghanami pollen (50:50) based on a factorial design in randomized complete blocks with three replications. To this end, the relative expression levels of Histone acetyltransferase HAC1-like (LOC103717600) (HAC 1), Arginine N-methyltransferase 6.1 (LOC103716582) (ANM), TIME FOR COFFEE-like (LOC103716450) (TFC), Homeobox-leucine zipper protein HOX9-like (LOC103703962) (HOX 9), MADS-box transcription factor 2-like (LOC103702602) (GLO 1), and MADS-box transcription factor 16 (LOC103701267) (DEF 1) genes were examined using qRT-PCR method in 3 times: (1) Before pollination, (2) 1 week after pollination, and (3) 2 weeks after pollination. The fruit yield was measured at 180 days after pollination (the end of the experiment). The results showed that the tissue culture palms had lower yield but higher relative genes expression in all pollen treatments compared to off-shoot palms. Off-shoot date palm pollinated with Red Ghanami pollen (5.93 tons/ha) showed the highest yield while the lowest yield was recorded in tissue culture date palm pollinated with Green Ghanami pollen (2.09 tons/ha). The relative expression of the six studied genes significantly increased in all treatments two weeks after pollination. Two weeks after all investigated treatments, the GLO gene had the highest expression, while the HAC gene showed the lowest relative expression. The relationship between the yield and the genes expression in the three studied times showed that the HOX gene had no considerable effect on flowering and yield. The ANM and TFC genes expression before pollination exhibited a positive relationship, whereas the expression of HAC, DEF, and GLO genes had a negative relationship with 1 week after pollination. Two weeks after pollination, the expression of the ANM gene revealed a significant positive relationship with the final yield of the date palm. Moreover, the correlation results showed the prominent role of the genes in different stages of growth and yield of date palms by influencing the expression of each other. In general, it is possible to improve the yield of the tissue culture date palm by affecting the expression of the genes in specific stages based on their function.

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Section: Molecular Studiesmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Effect of Pollen Treatments on Fruit Yield and Gene Expression in Offshoot and Tissue Culture Date Palms (Phoenix dactylifera L. cv. Barhi)

Alavipour,

Moallemi,

Khaleghi

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Abul-Soad et al (2002) reported that in 1970, shoot tip explants were utilized in micropropagation, however currently Abul-Soad (2011) and Solangi et al (2020) utilized explants of juvenile inflorescence for in vitro propagation of elite and rare date cultivars. Mirani et al (2019) and Mirani et al (2022) observed that in vitro propagation via inflorescence explants results in low percentage or no variations during fruiting. Keeping in view genotype, explant age, and auxincytokinin responses, the protocols can be exploited for other cultivar Abul-Soad and Al-Khayri, 2018).…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing Somatic Embryogenesis and Plantlet Regeneration of Date Palm using Immature Floral Buds

Solangi¹,

Jatoi²,

Abul-Soad³

et al. 2023

SJA

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Factors Influencing Somaclonal Variation in Date Palm, Detection and Selection for Application in the Plantation

Mirani,

Harikrishna,

Teo

et al. 2024

Somaclonal Variation: Basic and Practical Aspects

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Genetic stability analysis of tissue culture derived date palm cv. Dedhi plants using IRAP markers

Cited by 8 publications

References 23 publications

Effect of Pollen Treatments on Fruit Yield and Gene Expression in Offshoot and Tissue Culture Date Palms (Phoenix dactylifera L. cv. Barhi)

Effect of Pollen Treatments on Fruit Yield and Gene Expression in Offshoot and Tissue Culture Date Palms (Phoenix dactylifera L. cv. Barhi)

Factors Influencing Somatic Embryogenesis and Plantlet Regeneration of Date Palm using Immature Floral Buds

Factors Influencing Somaclonal Variation in Date Palm, Detection and Selection for Application in the Plantation

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