Genetic stability and phytochemical profiling of the in vitro regenerated plants of Angelica glauca Edgew.: an endangered medicinal plant of Himalaya

Rawat, Jiledar; Bhandari, Aakriti; Mishra, Susmita; Rawat, Balwant; Dhakad, Ashok Kumar; Thakur, Ajay; Chandra, Anup

doi:10.1007/s11240-018-1448-z

Cited by 22 publications

(11 citation statements)

References 25 publications

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“…Researchers often use molecular markers and ow cytometry to study the genetic stability of in vitro regenerated shoots. ISSR markers cover different genomic regions and offer an effective method for evaluating somaclonal variation in regenerated plants (Rawat et al 2018;Raji and Farajpour 2020). While preserving the genetics of the in vitro regenerants, the optimized protocol in the present study increased the accumulation of valuable bioactive compounds of ajowan medicinal plant using integrated SA-PEG biotic and abiotic elicitors.…”

Section: Discussionmentioning

confidence: 99%

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Niazian

Howyzeh

Sadat-Noori

2021

Plant Cell Tiss Organ Cult

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Bioactive compounds of medicinal plants have a wide range of applications in pharmaceutical, food and other industries. In vitro culture systems have great potential for sustainable production of bioactive compounds of medicinal plants. In the present study, the individual and combined effects of a stress tolerance-inducing (salicylic acid) and a stress-inducing elicitor (polyethylene glycol) were evaluated on regeneration e ciency, antioxidants activity and phytochemical pro le of in vitro shoot cultures of ajowan. Different concentrations of salicylic acid (SA) (0, 10, 20, 40, 80 µM) and polyethylene glycol (PEG 6000) (0, 1, 2, 5%) were added to the shoot regeneration Murashige and Skoog medium containing Kin (1.5 mg/L) and NAA (0.25 mg/L) plant growth regulators. Salicylic acid reduced the adverse effect of PEG treatment on number of regenerated shoots and in vitro rooting. The activities of catalase, superoxide dismutase, and peroxidase enzymatic antioxidants were signi cantly increased in SA + PEG treated plants. The gas chromatography-mass spectrometry (GC-MS)-pro ling revealed quantitative and qualitative phytochemical differences between control and SA + PEG treated plants. The greatest means of p-cymene and thymol bioactive compounds were obtained from in vitro shoots treated with 5% PEG + 40 µM SA. The inter-simple sequence repeats (ISSR) markers proved the genetic stability of in vitro regenerated plants. The presented protocol is useful for large-scale sustainable production of secondary metabolites (SMs) of medicinal plants. The same strategy (stress tolerance-inducing elicitor + stress-inducing elicitor) is applicable to increase valuable SMs in other production systems such as hydroponic, greenhouse and eld conditions.

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Section: Discussionmentioning

confidence: 99%

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Niazian

Howyzeh

Sadat-Noori

2021

Plant Cell Tiss Organ Cult

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“…Despite the fact that the species are widely traded illegally throughout the world, many regions lack conservation and management guidelines. Because of this, medicinal plants can be protected from a variety of hazards by efective in situ and ex situ conservation techniques [6,101]. To conserve this plant, researchers must establish propagation protocols, cultivation practices, and guidelines for the sustainable use of the rhizome.…”

Section: Conservation Statusmentioning

confidence: 99%

Traditional Uses, Active Ingredients, and Biological Activities of Paris polyphylla Smith: A Comprehensive Review of an Important Himalayan Medicinal Plant

Rawat

Pandey

Rawat

et al. 2023

Journal of Chemistry

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Paris polyphylla Smith (family: Melanthiaceae), a high-value medicinal herb endemic to the Himalayan region, has drawn much attention recently due to its immense use in the traditional healthcare system since ancient times. In the present review, an extensive database on P. polyphylla was systematically searched from databases such as Medline/PubMed, Scopus, the Web of Science, and the online service E-library.ru and SCImago (https://www.scimagojr.com/). Information on species, ecology, distribution, trade, ethnopharmacology, pharmacology, biotechnology, and molecular biology was gathered from 1979 to 2023 using 116 research publications. Major steroidal saponins such as Paris saponin I, V, VI, VII, and H have been found substantially effective in anticancer activity, abnormal uterine bleeding, dysfunctional uterine bleeding, and menorrhagia. Traditional breeding and propagation techniques cannot keep up with the world’s growing demand for herbal drugs. Therefore, it is critically necessary to take conservation measures and develop novel techniques for growing and cultivating this economically significant and highly valuable therapeutic herb. The advanced biotechnological approaches like micropropagation and genetic analysis introduced long back are either rare or lacking in the case of P. polyphylla. It contains a wealth of information that will serve as a baseline data source for various stakeholders, researchers working on various research aspects, and policymakers to define appropriate utilization and conservation plans for a high-value commercial medicinal plant called P. polyphylla. The review provides an updated overview and critical assessment of secondary data regarding the past and recent applications and interventions of P. polyphylla.

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“…The ultimate aim and the most crucial part of plant tissue culture is to produce genetically similar (true-to-type) plants concerning their mother plant [ 12 , 13 ]. Various molecular markers, such as RAPD, ISSR, SCoT, and others, can be used to determine genetic differences between regenerants and mother plants.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic homogeneity of in-vitro propagated apple root stock MM 104 using ISSR and SCoT primers

Bisht,

Rawat,

Gaira

et al. 2024

BMC Plant Biol

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Apple is an important fruit crop that is always in demand due to its commercial and nutraceutical value. Also, the requirement for quality planting material for this fruit crop for new plantations is increasing continuously. In-vitro propagation is an alternative approach, which may help to produce genetically identical high grade planting material. In this study, for the first time, an efficient and reproducible propagation protocol has been established for apple root stock MM 104 via axillary bud. Culturing axillary buds on Murashige and Skoog apple rootstock (MM 104) resulted in better in-vitro propagation. (MS) basal medium supplemented with 3.0% (w/v) sucrose and 0.8% (w/v) agar. The axillary buds were established in MS basal medium with BA (5.0 µM), NAA (1.0 µM) and further used to establish invitro propagation protocol. Plant Growth Regulators (PGRs), BA (1.0 µM) in combination with NAA (1.0 µM) was found most efficient for shoot multiplication (100%) and produced 9.8 shoots/explants with an average shoot length of (2.4 ± cm). All the shoots produced roots in 0.1 µM IBA with a 5-day dark period. Acclimatization of in-vitro raised plantlets was obtained with vermiculite: perlite: sand: soil (2:2:1:1) resulting in 76% survival under field conditions. The study showed that the use of axillary bud is efficient for multiple-shoot production of apple rootstock (MM 104). This is the first comprehensive report on in-vitro growth of apple root stock MM 104 with an assessment of genetic stability using DNA fingerprinting profiles based on Inter Simple Sequence Repeats (ISSR) and Start Codon Targeted (SCoT). The genetic stability of in-vitro-produced plants, as determined by SCoT and ISSR primers, demonstrated genetic closeness to the mother plant.

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Genetic stability and phytochemical profiling of the in vitro regenerated plants of Angelica glauca Edgew.: an endangered medicinal plant of Himalaya

Cited by 22 publications

References 25 publications

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Traditional Uses, Active Ingredients, and Biological Activities of Paris polyphylla Smith: A Comprehensive Review of an Important Himalayan Medicinal Plant

Assessment of genetic homogeneity of in-vitro propagated apple root stock MM 104 using ISSR and SCoT primers

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