Genetic stability in a predominating Turkish olive cultivar, Gemlik, assessed by RAPD, microsatellite, and AFLP marker systems

Abstract

Olive (Olea europaea L.) is an important fruit crop, with many health promoting properties being continuously discovered. World demand for both the fruit and olive oil is increasing in pace and hence countries try to meet the demand by disseminating 1 or 2 productive and adaptive cultivars to different regions formerly dominated by local cultivars. This study was performed to investigate the level of diversity in a very productive and adaptive cultivar, Gemlik, which has started to dominate most of the olive growing regions of Turkey. Three different marker systems, namely microsatellites (SSR), RAPD, and AFLP, with 15, 20, and 3 primer combinations, respectively, could not detect any genetic variation among 60 olive cv. Gemlik specimens sampled from 5 different regions, except several rare and random polymorphisms. The results showed that the expanding cultivar most possibly belonged to the clones of a single tree and revealed 100% sample identity.

Recent developments in olive (Olea europaea L.) genetics and genomics: applications in taxonomy, varietal identification, traceability and breeding

“…In other papers, RAPD markers have been considered to study the potential presence of intra-cultivar variability (Ç elikkol Akçay et al 2014;Figueiredo et al 2013;Leva and Petruccelli 2012), the genetic relationships between cultivars (Gomes et al 2012), the genetic characterisation of minor cultivars in local regions (Parra-Lobato et al 2012), the results of in vitro mutagenesis, and the detection of possible mutants in olive calli caused by the use of sodium azide (Alborzian Deh Sheikh and Moradnejad 2014).…”