Genetic stability of three economically important micropropagated banana (Musa spp.) cultivars of lower Indo-Gangetic plains, as assessed by RAPD and ISSR markers

Abstract

An efficient micropropagation protocol produced large number of plants of the three elite banana (Musa spp.) cultivars Robusta (AAA), Giant Governor (AAA) and Martaman (AAB) from shoot tip meristem. The genetic relationships and fidelity among the cultivars and micropropagated plants as assessed by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers, revealed three somaclonal variants from Robusta and three from Giant Governor. A total of 5330 RAPD and 2741 ISSR fragments were generated with 21 RAPD and 12 ISSR primers in micropropagated plants. The percentage of polymorphic loci by RAPD and ISSR were found to be 1.75, 5.08 in Robusta and 0.83, 5.0 in Giant Governor respectively. Among the two marker systems used, ISSR fingerprinting detected more polymorphism than RAPD in Robusta and Giant Governor with most of the primers showing similar fingerprinting profile, whereas Martaman revealed complete genetic stability.

Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch.

“…The genetic variation ranged from 0 to 5.6 % after 8 months of culture, which increased to 10 % after 10 years of culture. RAPD and ISSR markers have been used to assess the genetic stability of in vitro-derived plantlets of many other plant species (Ray et al 2006;Kishor and Devi 2009;Aggarval et al 2010;Bhatia et al 2011;Mohanty et al 2011). …”

Evaluation of Genetic Diversity in Some Banana Hybrids using ISSR Markers

“…The Inter Simple Sequence Repeats (ISSR) are DNA-based marker techniques that has been successfully used to determine genetic diversity and relationships Musa germplasms Kaemmer et al, (1992), Howell et al, (1994, Bhat and Jarret (1995), Crouch et al, (2000), Jain et al, (2007), Racharak and Eidathong (2007), Ruangsuttapha et al, (2007), Agoreyo et al,(2008), Brown et al,(2009) and for genome identification, Howell et al, (1994), Pillay et al, (2000), analysis of Musa breeding populations, Crouch et al, (1999), detection of somaclonal variants, Grajal-Martin et al, (1998) and genetic stability, Harirah and Khalid (2006), Ray et al, (2006), Lakshmanan et al, (2007) and Venkatachalam et al,(2007). Using these techniques an unlimited number of polymorphic bands can be produced with relative ease from minute amounts of genomic DNA, Welsh and McClelland(1990), Godwin et al, (1997), Reddy et al, (2002) allowing simultaneous screening of a large number of accessions.…”

“…ISSR and RAPD were used in determinate genetic stability of three economically important banana (Musa spp.) cultivars and showed that ISSR detected more polymorphism than RAPD, Ray et al, (2006). Similarly, ISSR were used for detection of genetic uniformity of micropropagated plantlets, Rout et al, (2009) and for screening in vitro mutagenesis and variance Khatri et al, (2011).…”

Genetic Fidelity Analysis in the Micropropagated Banana Derived from Immature Primordial Male Flower Bud

“…In banana, there are reports of somaclonal variation especially in cultivars Williams (AAA) (Damasco et al, 1996;Israeli et al, 1996 Robusta (AAA), and Giant Governor (AAA) (Ray, 2006) in micropropagated plants using different molecular markers. In present study, it was observed that ECS derived plantlets were genetically identical to sucker derived plants especially in cv.…”