Genetic structure among four populations of paddlefish, Polyodon spathula (Actinopterygii: Acipenseriformes: Polyodontidae), based on disomic microsatellite markers

Zheng, Xin; Schneider, Kyle J.; Lowe, Jeremiah D.; Gomelsky, Boris; Mims, Steven D.; Bu, Shuhai

doi:10.3750/aip2014.44.3.05

Cited by 7 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Given that microsatellites typically have more alleles and higher heterozygosity than SNPs (Tokarska et al 2009) and that evidence from other animal species have shown only weak or no congruence between the estimates of heterozygosity derived from the two marker types (Vignal et al 2002), we did not directly compare heterozygosity. Additionally, our Paddlefish inbreeding levels were generally consistent with a previous study in four geographically separate Paddlefish populations, with F is ranging from 0.013 (Tallapoosa River) to 0.099 (Yellowstone–Missouri River) (Zheng et al 2014). Overall, heterozygosity values were neither very low nor high, suggesting that fish in each river section have an intermediate level of genetic variability.…”

Section: Discussionsupporting

confidence: 90%

“…This study also represents the first population genetics assessment of Paddlefish using SNP markers. Other Paddlefish population studies have been conducted using allozymes, mitochondrial DNA, or microsatellite markers (Carlson et al 1982; Epifanio et al 1996; Heist and Mustapha 2008; Sloss et al 2009; Zheng et al 2014). For example, a previous genetic survey using seven microsatellite loci observed higher genetic heterozygosity values in Paddlefish populations from Mississippi (0.68) and Poland stocks (0.59–0.60, imported from USA) (Kaczmarczyk et al 2012) compared with this study with SNPs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Using fish hard‐part microchemistry and genetics to quantify population impacts of low‐use lock‐and‐dam structures on the Alabama River

et al. 2023

View full text Add to dashboard Cite

Objective: We used two approaches, fish hard-part microchemistry and genetics, to quantify effects of low-use lock-and-dam structures on riverine fish movement. Each approach varied in temporal scope, with microchemistry addressing effects within a lifetime and genetics addressing effects across generations. Methods:Water samples and individuals of two species (Paddlefish Polyodon spathula and Smallmouth Buffalo Ictiobus bubalus) were collected from four river sections that were separated by three low-use lock-and-dam structures on the Alabama River. Quarterly water samples were collected from 15 sites during 2017-2018, and concentrations of Sr, Ba, Mn, Mg, and Ca were quantified using mass spectrometry.Result: Water elemental signatures were spatially variable but temporally consistent.The Sr:Ca ratios in fish hard parts differed significantly among river sections for both species. Additionally, discriminant function analyses classified fish to their river capture section with accuracy between 55% and 74% for Paddlefish (errors nearly always assigned individuals to adjacent river sections) and 37-47% for Smallmouth Buffalo.Population genetic analyses included fish from each river section, as well as from Alabama River tributaries and a neighboring watershed. Genotyping-by-sequence techniques identified 1,889 and 3,737 single nucleotide polymorphisms postfiltering in Paddlefish and Smallmouth Buffalo, respectively, which we used to estimate population diversity indices and conduct differentiation analyses. Analysis of molecular variance, discriminant analysis of principal components, Bayesian clustering, and pairwise comparisons of F ST values indicated no strong evidence for genetic divergence in either species among river sections. Conclusion:Within-lifespan results based on hard-part microchemistry suggested a potential for population isolation. However, longer-term genetic effects were not apparent, possibly because the life span of these large and relatively long-lived species means that few generations have passed since dam construction, and there could be sufficient mixing or population connectivity to prevent genetic divergence across river sections, particularly at the most downstream structure.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Using fish hard‐part microchemistry and genetics to quantify population impacts of low‐use lock‐and‐dam structures on the Alabama River

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Based on the DNA analysis of paddlefish individuals, the controlled selection of spawner pairs can be performed in order to purposefully restore local populations and maintain genetic diversity of populations under artificial reproduction [11][12][13][14]. Additionally, the identification and assessment of genetic differences among the populations of natural reservoirs and farmed species will allow the establishment of phylogenetic relationships and determination of the origin of target objects for effective management of the genetic resources [13,[19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Genetic variation and phylogenetic relationships among domesticated and wild paddlefish (Polyodon spathula) populations

Kurta¹,

Malysheva²,

Skrypkina

2020

Biopolym. Cell

View full text Add to dashboard Cite

To determine the genetic variation and phylogenetic relationships among domesticated paddlefish populations from Ukraine and Poland with wild populations from the United States using microsatellite DNA markers. Methods. In total, 105 paddlefish individuals were analyzed using PCR technique, capillary electrophoresis, and statistical methods. Results. The evaluated mean number of alleles per locus (Na) varied from 5.5 to 6.6 in the Polish and Ukrainian paddlefish stocks, respectively, whereas twofold lower genetic polymorphism was demonstrated if compared to that found in wild population from the USA (11.1 alleles per locus). The mean observed (Ho) heterozygosity estimated in Ukrainian populations (0.709) was relatively close to those in the Polish (0.809) and wild (0.817) populations. Close genetic relationships were observed among the studied Ukrainian populations. The measured genetic similarity ranged from 0.908 (among populations from Kherson and Chernihiv regions) to 0.981 (among populations from Chernihiv and Vinnytsia regions). The Nei's genetic distances ranged from 0.019 (populations from Chernihiv and Vinnytsia regions) to 0.096 (populations from Kherson and Chernihiv regions). The constructed dendrogram demonstrated the genetic differences among hatchery-reared and natural paddlefish populations, which were separated into two single clusters according to their geographical origin. Conclusion. It has been revealed that Polish paddlefish population was more genetically distant from the studied Ukrainian populations of this species, which confirms the formation of Ukrainian and Polish populations from different parental individuals. The results obtained allow us to consider the investigated fish groups as a potential source of the exchange and enrichment of genetic resources of the paddlefish populations.

show abstract

“…Сучасні молекулярно-генетичні методи досліджень дозволяють ефективно проводити селекційно-племінну роботу щодо штучного відтворення веслоноса шляхом популяційно-генетичного моніторингу цінних генотипів та видової ідентифікації з використанням мікросателітних ДНК-маркерів [3][4][5][6]. Мікросателіти -це генетичні маркери з кодомінантним типом успадкування, які ефективно застосовуються для контролю за генетичною різноманітністю та еволюційними процесами у популяціях, а також для картування геномів в процесі роботи з рідкісними видами тварин [7][8][9].…”

unclassified

Development of a multiplex PCR for the genetic analysis of paddlefish (Polyodon spathula Walbaum,1792) populations

Kurta¹,

Malysheva²,

Spyrydonov³

2017

Ribogospod. nauka Ukr.

View full text Add to dashboard Cite

show abstract

Genetic structure among four populations of paddlefish, Polyodon spathula (Actinopterygii: Acipenseriformes: Polyodontidae), based on disomic microsatellite markers

Cited by 7 publications

References 13 publications

Using fish hard‐part microchemistry and genetics to quantify population impacts of low‐use lock‐and‐dam structures on the Alabama River

Using fish hard‐part microchemistry and genetics to quantify population impacts of low‐use lock‐and‐dam structures on the Alabama River

Genetic variation and phylogenetic relationships among domesticated and wild paddlefish (Polyodon spathula) populations

Development of a multiplex PCR for the genetic analysis of paddlefish (Polyodon spathula Walbaum,1792) populations

Contact Info

Product

Resources

About