Genetic structure and conservation status of Astragalus subrecognitus (Fabaceae): a very rare and narrow endemic species

Bagheri, Alireza; Abbasi, Shabnam; Mahmoodi, Mohammad; Roofigar, Azadeh Akhavan; Blattner, Frank R.

doi:10.5091/plecevo.2020.1648

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…CDDP molecular markers are a cutting-edge method that amplify the entire plant genome with primers based on conserved DNA sequences, producing bands and targets mostly related to biotic and abiotic stress. Many molecular markers, such as ISSR [15,16] and SRAP [17], have recently been used to examine phylogenetic relationships in Astragalus species. Aside from the utility of the previously mentioned markers, a new form of marker known as CDDP has emerged as a valuable tool for measuring diversity in various plant species [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Molecular Variation in Some Taxa of Genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region

Khal,

Tahir,

Abdul-Razaq

2023

Horticulturae

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Astragalus L. is one of the main genera of blossoming plants, and its diversity of species and forms is well known. The Astragalus L. taxa make a significant contribution to the mountainous and steppe ecosystems of the Kurdistan region of Iraq. Although the species within this genus have been the subject of several molecular studies, the evolutionary relationships among these species remain unidentified. Despite extensive research, little is known about the phylogenetic relationships among the various subgenera of Astragalus. This research is intended to investigate the molecular variation of 33 species of the genus Astragalus L. found in the Kurdistan area of Iraq. For molecular validations, three separate techniques (nuclear ribosomal DNA (rDNA), inter simple sequence repeats (ISSR), and conserved DNA-derived polymorphism (CDDP)) were used. With respect to Astragalus L. indentations, universal ITS1 and ITS4 gene sequencing was used, and the discovered sequences were subjected to BLAST searches in the NCBI database. A phylogenetic tree was generated with two main clades. Regarding detecting genetic diversity between the taxa, 24 molecular markers (14 ISSR and 10 CDDP) were used. High values of polymorphic materials and gene diversity were detected. ISSR markers had an average of 22.71 polymorphic bands per primer, while CDDP markers had the highest mean values for polymorphic information content (0.37), Shannon’s information index (0.27), expected heterozygosity (0.19), and unbiased expected heterozygosity (0.23). Cluster and principal coordinate analyses divided the Astragalus L. taxa into four main groups using the two molecular data sets. According to the results of the molecular variance analysis, the highest variation was detected within sections, with values of 92.01 and 89.48% for ISSR and CDDP markers, respectively. These outcomes suggest the effectiveness of molecular markers and the ITS region in determining and identifying genetic correlations between Astragalus species.

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Section: Introductionmentioning

confidence: 99%

Molecular Variation in Some Taxa of Genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region

Khal,

Tahir,

Abdul-Razaq

2023

Horticulturae

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“…Recently, different studies have widely applied these markers in the aquatic population (e.g., Chen et al, 2017;Hu et al, 2016;Butkuvienė et al, 2017) because they are more changeable and require less time and money compared to other molecular markers (Harris, 1999). The detection of the genetic variation in endangered species in nature is considered another application of ISSRs (Smith and Bateman, 2002;Kwiecińska-Poppe et al, 2020;Bagheri et al, 2020). Conversely, the sequencerelated amplified polymorphism (SRAP) aims to amplify the coding regions of DNA with primers targeting the ubiquitous motifs of open reading frames (Li and Quiros, 2001).…”

Section: Introductionmentioning

confidence: 99%

Genetic structure of Zannichellia L. populations according to mountain barriers in Iran as revealed by ISSR and SRAP markers

Abbasi¹,

Afsharzadeh²,

Dinarvand³

2021

Turk J Bot

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Zannichellia L. (Potamogetonaceae) is a widespread aquatic genus that is distributed in temperate and subtropical aquatic ecosystems worldwide. Because the biogeographical location of Iran includes mountain ranges and deserts that may cause different habitat types, it was assumed that these Zannichellia populations would be stronger differentiated than observed in Zannichellia from temperate regions. The present study aimed to test whether Z. palustris would demonstrate high levels of genetic differentiation over long distances and across mountain ranges. The genetic structure of the populations of Zannichellia was investigated using inter simple sequence repeats (ISSR) and sequence-related amplified polymorphism (SRAP) as molecular markers. The results demonstrated overall high levels of genetic differentiation among populations (GST = 0.53) and isolation by distance. Bayesian assignment analysis at the population level grouped individuals into eight genetic clusters (K = 8) that largely correspond to the topographic barriers. Alongside the same mountain ridge, the freshwater and saltwater habitat differences corresponded to Z. palustris var. palustris and Z. palustris var. pedicellata, respectively. Our results support the hypothesis of isolation by distance. This high level of genetic differentiation argues for a conservation strategy to maintain a diversity of populations of Zannichellia in mountainous regions.

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High genetic diversity in an endemic and vulnerable species: evidence from Astragalus cyclophyllon (Fabaceae) in Iran

Aliabadi

Bagheri

Abbasi

et al. 2023

Genet Resour Crop Evol

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The genus Astragalus L. with about 3000 species is the largest genus of flowering plants. It is also the largest genus in the flora of Iran with more than 850 taxa. Astragalus cyclophyllon Beck is an endemic taxon belonging to Astragalus sect. Incani DC. It occurs scattered in the steppe and semi-steppe areas of the western and central parts of Iran. This species is one of the most important forage taxa of Astragalus. According to IUCN criteria it has been classified as a vulnerable taxon in Iran, as it occurs in habitats intensely used by herders for their livestock. Here we evaluate the genetic diversity of the populations of this species in Iran to see whether genetic diversity is high or if reductions have already happened. For this purpose, sampling was done in all geographical areas with A. cyclophyllon populations in Iran. In total, 80 individuals representing 29 populations were studied using 33 quantitative and qualitative morphological characters and 10 inter-simple sequence repeat (ISSR) primers. ISSR revealed 240 bands which all were polymorphic. Neighbor-joining cluster analysis divided the individuals in four groups, Principal Coordinate Analysis and Bayesian population assignment analysis in Structure resulted in three genetic units. Morphological variation showed no correlation with the molecular data. The mean of Gst and Nm indices are 0.516 and 0.468, respectively, which indicate a very high genetic differentiation and low gene flow between the studied populations. According to these results, we conclude that genetic diversity is high in this species and that, therefore, the major threat for A. cyclophyllon is currently not related to inbreeding depression in populations, but might be due to livestock grazing that could change the population demographic structure by reducing regular establishment of new offspring.

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Genetic structure and conservation status of Astragalus subrecognitus (Fabaceae): a very rare and narrow endemic species

Cited by 5 publications

References 24 publications

Molecular Variation in Some Taxa of Genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region

Molecular Variation in Some Taxa of Genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region

Genetic structure of Zannichellia L. populations according to mountain barriers in Iran as revealed by ISSR and SRAP markers

High genetic diversity in an endemic and vulnerable species: evidence from Astragalus cyclophyllon (Fabaceae) in Iran

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