Genetic structure and fungicide resistance profile of Botrytis spp. populations causing postharvest gray mold of pomegranate fruit in Greece and California

Testempasis, Stefanos; Puckett, Ryan D; Michailides, Themis J.; Karaoglanidis, G. S.

doi:10.1016/j.postharvbio.2020.111319

Cited by 13 publications

(14 citation statements)

References 35 publications

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“…When comparing genetic diversity between the two marker systems, the 496 SNPs were only able to distinguish an additional two to three MLGs for the locations with the highest number (Southwest 1 and Southwest 2), but with the two lower MLG locations (West and Northwest), more than six additional MLGs were detected. While both types of markers were able to differentiate population structure at the multiple fungicide resistance level, thiabendazole and boscalid resistance, neither marker system was able to detect significant differentiation based on location similar to other Botrytis studies (Hu et al, 2018;Delong et al, 2020;Testempasis et al, 2020). Minor differences associated with region were detected with microsatellite markers, but were not significant at the p = 0.05 level.…”

Section: Discussionmentioning

confidence: 58%

“…This variability could be, in part, caused by regional differences in Botrytis population composition. Fungicide spray programs and the resulting resistance have repeatedly been shown to play a large role in Botrytis field population composition, but smaller differences caused by host, season, or marker resolution may also play a role (Wessels et al, 2016;Kozhar et al, 2020;Testempasis et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…population diversity (Kretschmer and Hahn, 2008;Fekete et al, 2012;Wessels et al, 2016;Hu et al, 2018). Previous studies that used TEs or microsatellites as markers for population differentiation showed that different hosts can be dominated by different populations such as grape and pomegranate, and were dominated by transposa isolates that have both TEs (Váczy et al, 2008;Johnston et al, 2014;Delong et al, 2020;Testempasis et al, 2020). The fungicide resistance profile is different between different strains of Botrytis (Martínez et al, 2008;Leroux et al, 2010;Johnston et al, 2014;Delong et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Population Genetic Analyses of Botrytis cinerea Isolates From Michigan Vineyards Using a High-Throughput Marker System Approach

et al. 2021

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As sequencing costs continue to decrease, new tools are being developed for assessing pathogen diversity and population structure. Traditional marker types, such as microsatellites, are often more cost effective than single-nucleotide polymorphism (SNP) panels when working with small numbers of individuals, but may not allow for fine scale evaluation of low or moderate structure in populations. Botrytis cinerea is a necrotrophic plant pathogen with high genetic variability that can infect more than 200 plant species worldwide. A panel of 52 amplicons were sequenced for 82 isolates collected from four Michigan vineyards representing 2 years of collection and varying fungicide resistance. A panel of nine microsatellite markers previously described was also tested across 74 isolates from the same population. A microsatellite and SNP marker analysis of B. cinerea populations was performed to assess the genetic diversity and population structure of Michigan vineyards, and the results from both marker types were compared. Both methods were able to detect population structure associated with resistance to the individual fungicides thiabendazole and boscalid, and multiple fungicide resistance (MFR). Microsatellites were also able to differentiate population structure associated with another fungicide, fluopyram, while SNPs were able to additionally differentiate structure based on year. For both methods, AMOVA results were similar, with microsatellite results explaining a smaller portion of the variation compared with the SNP results. The SNP-based markers presented here were able to successfully differentiate population structure similar to microsatellite results. These SNP markers represent new tools to discriminate B. cinerea isolates within closely related populations using multiple targeted sequences.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Population Genetic Analyses of Botrytis cinerea Isolates From Michigan Vineyards Using a High-Throughput Marker System Approach

et al. 2021

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“…Concerning Botrytis , different species within this genus can cause gray mold. Testempasis et al [ 72 ] reported that B. cinerea , B. pseudocinerea , and B. cinerea group S were observed on pomegranates grown in Greece and California. Among these, B. cinerea was the most abundant [ 72 ], confirming the results from this research.…”

Section: Discussionmentioning

confidence: 99%

“…Testempasis et al [ 72 ] reported that B. cinerea , B. pseudocinerea , and B. cinerea group S were observed on pomegranates grown in Greece and California. Among these, B. cinerea was the most abundant [ 72 ], confirming the results from this research. B. cinerea is considered the main postharvest fungal pathogen of pomegranate [ 17 , 19 ] due to the large economic losses that it causes [ 20 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Postharvest Rot of Pomegranate Fruit in Southern Italy: Characterization of the Main Pathogens

Mincuzzi

Sanzani

Palou

et al. 2022

JoF

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Pomegranate (Punica granatum L.) is an emerging crop in Italy and particularly in southern regions, such as Apulia, Basilicata, and Sicily, due to favorable climatic conditions. The crop is affected by several pathogenic fungi, primarily in the field, but also during postharvest phases. The most important postharvest fungal diseases in pomegranate are gray and blue molds, black heart and black spot, anthracnose, dry rot, and various soft rots. The limited number of fungicides allowed for treatment in the field and the lack of postharvest fungicides make it difficult to control latent, quiescent, and incipient fungal infections. Symptomatic pomegranates from southern Italy were sampled and isolated fungi were morphologically and molecularly characterized. The data obtained revealed that various species of Penicillium sensu lato (including Talaromyces genus), Alternaria spp., Coniella granati, and Botrytis cinerea were the principal etiological agents of postharvest pomegranate fruit diseases; other relevant pathogens, although less represented, were ascribable to Aspergillus sect. nigri, Colletotrichum acutatum sensu stricto, and Cytospora punicae. About two thirds of the isolated pathogens were responsible for latent infections. The results obtained may be useful in planning phytosanitary control strategies from the field to storage, so as to reduce yield losses.

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Genetic structure and pyrimethanil resistance of Botrytis spp. causing gray mold on strawberry from greenhouses in Zhejiang, China

Zhao,

Chen,

Mao

et al. 2024

Pesticide Biochemistry and Physiology

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Genetic structure and fungicide resistance profile of Botrytis spp. populations causing postharvest gray mold of pomegranate fruit in Greece and California

Cited by 13 publications

References 35 publications

Population Genetic Analyses of Botrytis cinerea Isolates From Michigan Vineyards Using a High-Throughput Marker System Approach

Population Genetic Analyses of Botrytis cinerea Isolates From Michigan Vineyards Using a High-Throughput Marker System Approach

Postharvest Rot of Pomegranate Fruit in Southern Italy: Characterization of the Main Pathogens

Genetic structure and pyrimethanil resistance of Botrytis spp. causing gray mold on strawberry from greenhouses in Zhejiang, China

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