Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily

Panno, Stefano; Caruso, A.; Bertacca, Sofia; Pisciotta, Antonino; Lorenzo, Rosario Di; Marchione, S.; Matić, S.; Davino, Salvatore

doi:10.3390/agriculture11060496

Cited by 5 publications

(4 citation statements)

References 44 publications

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“…The introduction of new sensitive and economic diagnostic techniques mentioned above may contribute to further minimizing sanitary risks in the production of both local grape and wine industry, and could be applied for territory monitoring, disease spread [71], and to evaluate the genetic diversity and molecular variability of these pathogens [72].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability

et al. 2022

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Background: the most widely distributed and virulent Grapevine leafroll-associated viruses (GLRaV) that affect grapevine are GLRaV-1 and GLRaV-3, transmitted semi-persistently by different mealybugs and soft scales, mainly causing downward rolling of the leaf margins and interveinal reddening. Methods: the main objectives of this study were to investigate the genetic structure and molecular diversity of GLRaV-1 and GLRaV-3 in 617 samples from 11 autochthonous Sicilian grapevine cultivars, ascertaining their presence and spread. The detection was implemented by serological and molecular analyses and subsequently phylogenetic analyses on selected Sicilian isolates were conducted. Results: in total, 33 and 138 samples resulted positive to GLRaV-1 and GLRaV-3, with an incidence of 5.34% and 22.36%, respectively; 9 out of the 11 cultivars resulted positive, while the presence of both viruses was not found in ‘Grillo’ and ‘Moscato’ cultivars. Conclusions: phylogenetic analyses of the coat protein (CP) gene of 12 GLRaV-1 selected sequences showed a close relationship with European isolates; the discrete nucleotide differentiation and positive selection could demonstrate a current increase in population fitness. The phylogenetic analyses of the CP gene of 31 GLRaV-3 Sicilian CP sequences demonstrates a close relationship between Sicilian and different countries isolates; a certain stability of GLRaV-3 in the different cultivars analyzed is suggested by the discrete differentiation nucleotide and negative selection of the Sicilian isolates.

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Section: Discussionmentioning

confidence: 99%

Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability

et al. 2022

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“…Moreover, the extensive use of Vitis rupestris Scheele in the last century as rootstock, imported from America, that allowed the eradication of phylloxera problems, and the use of uncertified propagative material within Italian territory, probably favored the wide spread of several viruses in almost all areas of grapevine cultivation, also explaining the presence of GVA infections in the autochthonous Sicilian cultivars [60].…”

Section: Discussionmentioning

confidence: 99%

“…In this scenario, it is important to monitor the territory, the sanitary status of plant propagation material, the disease spread, the genetic diversity and molecular variability of these pathogens [60,64,65], thus reducing the production for the livelihood of both the local grape and wine industries.…”

Section: Discussionmentioning

confidence: 99%

Evolutionary Analysis of Grapevine Virus A: Insights into the Dispersion in Sicily (Italy)

et al. 2022

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Grapevine virus A (GVA) is a phloem-restricted virus (genus Vitivirus, family Betaflexiviridae) that cause crop losses of 5–22% in grapevine cultivars, transmitted by different species of pseudococcid mealybugs, the mealybug Heliococcus bohemicus, and by the scale insect Neopulvinaria innumerabilis. In this work, we studied the genetic structure and molecular variability of GVA, ascertaining its presence and spread in different commercial vineyards of four Sicilian provinces (Italy). In total, 11 autochthonous grapevine cultivars in 20 commercial Sicilian vineyards were investigated, for a total of 617 grapevine samples. Preliminary screening by serological (DAS-ELISA) analysis for GVA detection were conducted and subsequently confirmed by molecular (RT-PCR) analysis. Results showed that 10 out of the 11 cultivars analyzed were positive to GVA, for a total of 49 out of 617 samples (8%). A higher incidence of infection was detected on ‘Nerello Mascalese’, ‘Carricante’, ‘Perricone’ and ‘Nero d’Avola’ cultivars, followed by ‘Alicante’, ‘Grecanico’, ‘Catarratto’, ‘Grillo’, ‘Nerello Cappuccio’ and ‘Zibibbo’, while in the ‘Moscato’ cultivar no infection was found. Phylogenetic analyses carried out on the coat protein (CP) gene of 16 GVA sequences selected in this study showed a low variability degree among the Sicilian isolates, closely related with other Italian isolates retrieved in GenBank, suggesting a common origin, probably due to the exchange of infected propagation material within the Italian territory.

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“…GFLV belongs to the Secoviridae family, and it is considered mainly responsible for grapevine fanleaf disease (GFD), which is one of the most severe virus diseases of grapevines [7]. Nepoviruses involved in fanleaf degeneration can cause symptoms such as leaf distortion, yellow mosaic close to primary veins, bright yellow vein banding on leaves, double nodes, and short and malformed internodes [8]. This depends mainly by the virulence of the virus isolate, the susceptibility of the grapevine variety, and environmental factors [9].…”

Section: Introductionmentioning

confidence: 99%

Detection of Ampelovirus and Nepovirus by Lab-on-a-Chip: A Promising Alternative to ELISA Test for Large Scale Health Screening of Grapevine

et al. 2022

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The Ampelovirus Grapevine leafroll-associated virus 3 (GLRaV-3) and the Nepovirus Grapevine fanleaf virus (GFLV) are pathogens reported in many grapevine-growing areas all over the world, main causal agents of grapevine leafroll disease and grapevine fanleaf disease, respectively. Prevention of virus spread thanks to rapid diagnosis of infected plants is a key factor for control of both diseases. Although serological (e.g., enzyme-linked immunosorbent assay-ELISA test) and molecular methods are available to reveal the presence of the viruses, they turn out to be quite expensive, time-consuming and laborious, especially for large-scale health screening. Here we report the optimization of a lab-on-a-chip (LOC) for GLRaV-3 and GFLV detection, based on an electrochemical transduction and a microfluidic multichamber design for measurements in quadruplicate and simultaneous detection of both targets. The LOC detect GLRaV-3 and GFLV at dilution factors more than 15 times higher than ELISA, providing a higher sensitivity in the detection of both viruses. Furthermore, the platform offers several advantages as easy-to-use, rapid-test, portability and low costs, favoring its potential application for large-scale monitoring programs. Compared to other grapevine virus biosensors, our sensing platform is the first one to provide a dose-dependent calibration curve combined with a microfluidic module for sample analysis and a portable electronics providing an operator-independent read-out scheme.

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Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily

Cited by 5 publications

References 44 publications

Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability

Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability

Evolutionary Analysis of Grapevine Virus A: Insights into the Dispersion in Sicily (Italy)

Detection of Ampelovirus and Nepovirus by Lab-on-a-Chip: A Promising Alternative to ELISA Test for Large Scale Health Screening of Grapevine

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