Genetic Structure and Temporal Dynamics of a <i>Colletotrichum graminicola</i> Population in a Sorghum Disease Nursery

Rosewich, U. Liane; Pettway, R. E.; McDonald, Bruce A.; Duncan, R. R.; Frederiksen, R. A.

doi:10.1094/phyto.1998.88.10.1087

Cited by 61 publications

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References 19 publications

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“…The two sites were less than 50 km apart, and the high level of differentiation implies that there were barriers to random gene flow between the populations. Rosewich et al (1998) studied the genetic structure and temporal dynamics of a C. graminicola population from a sorghum nursery in Georgia, U.S.A., and concluded that genetic drift and gene flow were not major contributors to genetic structure, while asexual reproduction had a significant effect. Rosewich et al (1998) found very low levels of genotypic diversity among the C. graminicola isolates from sorghum, with an overall normalized value of 0.0037.…”

Section: Discussionmentioning

confidence: 99%

Population structure and mating-type genes ofColletotrichum graminicolafromAgrostis palustris

Chen

Khan²,

Hsiang³

2002

Can. J. Microbiol.

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Eighty-seven isolates of Colletotrichum graminicola, mostly from Agrostis palustris, were collected in grass fields, most of which were in Ontario, Canada. Specific primers were designed to amplify the mating-type (MAT) genes and, among 35 isolates tested, all yielded a band of the expected size for MAT2. For six isolates, the MAT2 PCR products were sequenced and found to be similar to that reported for MAT2 of C. graminicola from maize. Based on 119 polymorphic bands from 10 random amplified polymorphic DNA primers, analyses of genetic distances were found to generally cluster isolates by host and geographic origin. Among 42 isolates from a grass field in Ontario, significant spatial autocorrelation was found to occur within a 20-m distance, implying that this is the effective propagule dispersal distance. Although clonal propagation was observed in the 87 isolates with 67 unique genotypes, the extent of genetic variation in local populations implies some occurrence of sexual or asexual recombination.Key words: anthracnose, turfgrass, RAPD, MAT, reproduction, fungi.Résumé : Quatre-vingt-sept isolats de Colletotrichum graminicola, la plupart du temps à partir d'Agrostis palustris, ont été récoltés dans des champs d'herbes, principalement en Ontario, Canada. Des amorces spécifiques ont été contruites pour amplifier les gènes des différents types de croisement (MAT) et, parmi 35 isolats vérifiés, tous produisaient une bande dont la taille correspondait à celle de MAT2. Chez six isolats, les produits PCR MAT2 ont été séquencés et se sont révélés semblables à ceux connus pour MAT2 de C. graminicola du maïs. En se basant sur 119 bandes de polymorphisme obtenues avec 10 amorces d'ADN polymorphe amplifié au hasard, la mesure des distances parcourues par les gènes a permis de regrouper de façon générale les isolats selon l'hôte et la région géographique. Avec 42 isolats provenant d'un même champ, nous avons observé une autocorrélation spatiale significative dans les limites de 20 m, ce qui serait donc la distance maximale de la dispersion des propagules. Même si une propagation des clones a été observée chez les 87 isolats qui portaient 67 génotypes uniques, l'importance des variations génétiques dans les populations locales suggère la présence de phénomènes de recombinaison sexuée ou asexuée.

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Section: Discussionmentioning

confidence: 99%

Population structure and mating-type genes ofColletotrichum graminicolafromAgrostis palustris

Chen

Khan²,

Hsiang³

2002

Can. J. Microbiol.

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“…The same results were reported in the study conducted by White et al (1979) where the authors found that the significant yield reduction due to anthracnose stalk rot was highly dependent on genotype and environment. Rosewich et al (1998) found that asexual reproduction at a location may give rise in a predominance of one race that is occasionally influenced by genetic drift and gene flow. Thakur et al (2007) reported differential severity among the lines under study, years and locations.…”

Section: Effects Of Genotype By Environment Interaction On Resistancementioning

confidence: 99%

Sorghum [Sorghum bicolor (L.) Moench] breeding for resistance to leaf and stalk anthracnose, Colletotrichum sublineolum, and improved yield: Progress and prospects

Mofokeng¹,

Shimelis²,

Laing³

et al. 2017

Aust J Crop Sci

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Sorghum is one of the main staple food crops for millions of subsistence farmers in Africa. Biotic and abiotic challenges are the major production constraints of the crop. Amongst the sorghum biotic constraints, anthracnose is the major devastating disease causing up to 80% of yield reduction. The productivity and profitability of sorghum is limited by several biotic constraints, most notably anthracnose caused by the aggressive fungal pathogen Colletotrichum sublineolum. The most effective and environmentally responsible strategy to control anthracnose is through the incorporation of resistance genes. However, although several sources have been identified, the lack of information with regard to its genetic control of resistance has limited their adequate use in breeding programs. Additionally, the limitations of breeding regarding the leaf and stalk anthracnose resistance and also the need for evaluating materials for resistance and yield in different environments is of major importance. There is limited information about the combining ability, gene action and genetic effects and relationships between anthracnose resistance and grain yield which is required in devising appropriate strategies for developing resistant and high yielding sorghum varieties. This review provides theoretical basis of the progress and challenges for breeding sorghum for anthracnose resistance and improved yield.

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“…The disease occurs worldwide, but is more commonly observed in tropical or subtropical environments where frequent rainfall, high relative humidity and warm temperatures enhance the development and spread of the disease (Casela et al, 2001;Hess et al, 2002;Marley et al, 2001;Néya and Le Normand, 1998;Ngugi et al, 2002;Thakur and Mathur, 2000;Thomas et al, 1996;Valério et al, 2005). In the United States, anthracnose is more prevalent in the Southern Plains and Southeastern States (Rosewich et al, 1998;Cardwell et al, 1989). Sorghum anthracnose will typically appear on infected leaves approximately 30 to 40 days after seedling emergence; although, infection can occur at every stage of plant development.…”

Section: Introductionmentioning

confidence: 99%

Field Assessment of Anthracnose Disease Response for the Sorghum Germplasm Collection from the Mopti Region

Erpelding¹

2010

American Journal of Agricultural and Biological Sciences

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Problem statement: Sorghum anthracnose (Colletotrichum sublineolum) is a highly variable pathogen and new sources of host plant resistance are required for the development of resistant sorghum varieties. Germplasm collections are an important source of host plant resistance and screening germplasm will be essential to identify new sources of resistance. Approach: The sorghum (Sorghum bicolor) collection from the Mopti region of Mali was inoculated with Colletotrichum sublineolum and evaluated for foliar anthracnose disease response in Isabela, Puerto Rico during the 2004 and 2005 growing seasons using a partially balanced lattice design with three replications. Results: A resistant response was observed for 45 of the 97 accessions in the collection and mean infection severity for the 52 susceptible accessions was 27.6%. An association was observed between resistance and the administrative district where the germplasm was collected. More than 50% of the accessions from the Bandiagara and Bankass districts showed a resistant response. The lowest frequency of resistant germplasm was observed for the Mopti district with 25% of the accessions showing a resistant response. The susceptible accessions from the Mopti district, however, showed the lowest mean infection severity. Approximately 44% of the accessions from the Douentza district showed a resistant response with the susceptible accessions showing the highest mean infection severity. These results suggest an association between annual rainfall and anthracnose resistance, with sorghum accessions from drier regions showing greater susceptibility. Anthracnose resistance also showed an association with sorghum race classification and race guinea accessions were more frequently resistant as compared to accessions classified as race durra or durra-bicolor. Conclusion: The results indicated that anthracnose resistant sorghum germplasm is frequent in the Mopti region of Mali and that ecogeographic origin and sorghum characterization information can be used to aid in germplasm selection or germplasm acquisition to identify anthracnose resistant sources.

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Genetic Structure and Temporal Dynamics of a Colletotrichum graminicola Population in a Sorghum Disease Nursery

Cited by 61 publications

References 19 publications

Population structure and mating-type genes ofColletotrichum graminicolafromAgrostis palustris

Population structure and mating-type genes ofColletotrichum graminicolafromAgrostis palustris

Sorghum [Sorghum bicolor (L.) Moench] breeding for resistance to leaf and stalk anthracnose, Colletotrichum sublineolum, and improved yield: Progress and prospects

Field Assessment of Anthracnose Disease Response for the Sorghum Germplasm Collection from the Mopti Region

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