Genetic Structure of an Endemic Japanese Conifer, Sciadopitys verticillata (Sciadopityaceae), by Using Microsatellite Markers

Kawase, Daiju; Tsumura, Yoshihiko; Tomaru, Nobuhiro; Seo, Akihiro; Yumoto, Takakazu

doi:10.1093/jhered/esp128

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…However, there are some conifers, such as S . verticillata ( h varied from 0.282 to 0.450) [26] and N . longibracteata ( h varied from 0.291 to 0.851) [27], displaying relatively higher level of genetic diversity, which reflecting the long-term divergence by different local environmental conditions or climate change occurred in the coniferous species.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers

Yu¹,

Peng²,

Chao³

et al. 2017

PLoS ONE

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Genetic diversity and variation among 11 populations of Chinese fir from Fujian province and Taiwan were assessed using inter-simple sequence repeat (ISSR) markers to reveal the evolutionary relationship in their distribution range in this report. Analysis of genetic parameters of the different populations showed that populations in Fujian province exhibited a greater level of genetic diversity than did the populations in Taiwan. Compared to Taiwan populations, significant limited gene flow were observed among Fujian populations. An UPGMA cluster analysis showed that the most individuals of Taiwan populations formed a single cluster, whereas 6 discrete clusters were formed by each population from Fujian. All populations were divided into 3 main groups and that all 5 populations from Taiwan were gathered into a subgroup combined with 2 populations, Dehua and Liancheng, formed one of the 3 main groups, which indicated relative stronger relatedness. It is supported by a genetic structure analysis. All those results are suggesting different levels of genetic diversity and variation of Chinese fir between Fujian and Taiwan, and indicating different patterns of evolutionary process and local environmental adaption.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers

Yu¹,

Peng²,

Chao³

et al. 2017

PLoS ONE

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“…On the Japanese islands, it is part of mixed conifer-angiosperm forests where it occurs as solitary trees or in small monotypic stands (Farjon 2005a(Farjon , 2010. Sciadopitys verticillata currently grows at altitudes of 200-1700 m under a humid cool to warm temperate climate and is often found in rocky, cool and moist ravines and valleys in mountainous areas (Iwatsuki et al, 2000;Farjon 2005aFarjon , 2010Kawase et al, 2010). Even though the extant S. verticillata is only growing in mountainous areas today, it seems that it was also part of the lowland vegetation during the Cenozoic.…”

Section: Genus Tsuga Carrièrementioning

confidence: 99%

Combined LM and SEM study of the Middle Miocene (Sarmatian) palynoflora from the Lavanttal Basin, Austria: Part II. Pinophyta (Cupressaceae, Pinaceae and Sciadopityaceae)

Grímsson

Zetter

2011

Grana

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“…Despite the wide application of nSSRs, examples on the application of SSR multiplexing in conifers remain scarce (Devey et al 2002;Bell et al 2004;Karhu et al 2006;Gugerli et al 2009;Salzer et al 2009;Kawase et al 2010;Wagner et al 2012). In the present work, we developed two multiplexes on a set of nuclear SSR loci (nSSRs) previously described in the pine literature, which we pre-selected from a large collection of SSRs based on their individual performance in Scots pine.…”

Section: Introductionmentioning

confidence: 99%

Development and transferability of two multiplexes nSSR in Scots pine (Pinus sylvestris L.)

et al. 2015

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Single sequence repeat (SSR) multiplexing is a semi high-throughput PCR methodology for the analysis of multiple SSRs. We developed two SSR multiplexes selected from SSR loci previously reported in the pine literature and tested the transferability of both SSR multiplexes in nine other pine species. We tested 234 nuclear SSR loci (nSSRs) previously described in the pine literature and selected ten nSSRs following the simple criteria of interpretability and reproducibility. Selected nuclear loci were divided into two nSSRs multiplex sets and their amplification was optimized for three different multiplex PCR methods based on: (a) a custom PCR protocol, (b) a custom protocol with hotstart taq polymerase, and (c) a commercially available kit for SSR multiplexing. To validate their performance, the level of genetic diversity was assessed in three Scots pine natural populations (Hungary, northern Sweden and southern Sweden). In addition, we also tested the transferability of these multiplexes in nine other pine species. We have developed two nSSRs multiplexes of five loci each that will contribute to reduce the costs of nSSR scoring, while increasing the capacity of nSSR loci analysis. Amplification was successful in all three populations (94 % success) and the level of polymorphism (7.1 alleles/marker) was similar to that previously reported for other Scots pine natural populations. Transferability of both multiplexes was successful for those pine species closely related to Scots pine.

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Genetic Structure of an Endemic Japanese Conifer, Sciadopitys verticillata (Sciadopityaceae), by Using Microsatellite Markers

Cited by 13 publications

References 25 publications

Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers

Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers

Combined LM and SEM study of the Middle Miocene (Sarmatian) palynoflora from the Lavanttal Basin, Austria: Part II. Pinophyta (Cupressaceae, Pinaceae and Sciadopityaceae)

Development and transferability of two multiplexes nSSR in Scots pine (Pinus sylvestris L.)

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