Genetic structure of deltamethrin-resistant populations of Triatoma infestans (Hemiptera: Reduviidae) in the Gran Chaco

Piccinali, Romina Valeria; Fronza, Georgina; Mougabure‐Cueto, Gastón; Toloza, Ariel Ceferino

doi:10.1007/s00436-020-06789-y

Cited by 10 publications

(8 citation statements)

References 51 publications

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“…Interestingly, a silent substitution from thymidine to cytosine was detected in 63% of the mapped reads (base pair 183 in S3 Fig) . This substitution was also found in high proportion in a resistant population from a neighbouring locality, which carries the non-silent kdr mutation L925I, and in low proportion in the susceptible population [8]. In addition, a microsatellite study performed in the zone proposed some degree of gene flow between CC and a near high-resistant locality [75]. This reinforces the hypothesis that the ancestral T. infestans populations from the region had a common genetic background.…”

Section: Analysis Of Target-site Mutations Associated To Pyrethroid Resistancesupporting

confidence: 68%

Transcriptomic modulation in response to an intoxication with deltamethrin in Triatoma infestans, vector of Chagas disease

Traverso

Estivalis

Fernandes

et al. 2021

Preprint

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Background: Triatoma infestans is the main vector of Chagas disease in the Southern Cone. The resistance to pyrethroid insecticides developed by populations of this species impairs the effectiveness of vector control campaigns in wide regions of Argentina. The study of the global transcriptomic response to pyrethroid insecticides is important to deepen the knowledge about detoxification in triatomines. Methodology and findings: We used RNA-Seq to explore the early transcriptomic response of T. infestans after intoxication with deltamethrin. We were able to assemble a complete transcriptome of this vector and found evidence of differentially expressed genes belonging to diverse families such as chemosensory and odorant-binding proteins, ABC transporters and heat-shock proteins. Moreover, genes related to transcription and translation, energetic metabolism and cuticle rearrangements were also modulated. Finally, we characterized the repertoire of previously uncharacterized detoxification-related gene families in T. infestans and Rhodnius prolixus. Conclusions and significance: Our work contributes to the understanding of the detoxification response in vectors of Chagas disease. Given the absence of genomic information from T. infestans, the analysis presented here constitutes a resource for molecular and physiological studies in this species. The results increase the knowledge on detoxification processes in vectors of Chagas disease, and provide relevant information to explore new potential insecticide resistance mechanisms in these insects.

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Section: Analysis Of Target-site Mutations Associated To Pyrethroid Resistancesupporting

confidence: 68%

Transcriptomic modulation in response to an intoxication with deltamethrin in Triatoma infestans, vector of Chagas disease

Traverso

Estivalis

Fernandes

et al. 2021

Preprint

Self Cite

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“…And within this context, domestic populations are assumed to have been the subject of greater selection pressures, compared to their sylvatic counterparts and therefore be less diverse. Recent studies of T. infestans populations in the Argentinean Chaco support some of these assumptions, with pyrethroid resistant individuals characterized by lower levels of genetic diversity than to their resistant counterparts (Piccinali et al, 2020).…”

Section: Discussionmentioning

confidence: 97%

Insights into the evolution and dispersion of pyrethroid resistance among sylvatic Andean Triatoma infestans from Bolivia

Marcet

Santo-Orihuela

Messenger

et al. 2021

Infection, Genetics and Evolution

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Sylvatic populations of Triatoma infestans represent a challenge to Chagas disease control as they are not targeted by vector control activities and may play a key role in post-spraying house re-infestation.Understanding sylvatic foci distribution and gene flow between sylvatic and domestic populations is crucial to optimize vector control interventions and elucidate the development and spread of insecticide resistance. Herein, the genetic profiles of five Andean T. infestans populations from Bolivia with distinct insecticide susceptibility profiles were compared. Multilocus genotypes based on eight microsatellites and the DNA sequence of a fragment of the cytochrome B (cytB) gene were obtained for 92 individuals. CytB haplotypes were analyzed with previously reported Bolivian T. infestans haplotypes to evaluate putative historical gene flow among populations. Each specimen was also screened for two nucleotide mutations in the sodium channel gene (kdr), related to pyrethroid resistance (L1014 and L9251). Significant genetic differentiation was observed among all populations, although individuals of admixed origin were detected in four of them. Notably, the genetic profiles of adjacent domestic and sylvatic populations of Mataral, characterized by higher levels of insecticide Journal Pre-proof resistance, support their common ancestry. Only one sylvatic individual from Mataral carried the kdr mutation L1014, suggesting that this mechanism is unlikely to cause the altered insecticide susceptibility observed in these populations. However, as the resistance mutation is present in the area, it has the potential to be selected under insecticidal pressure. Genetic comparisons of these populations suggest that insecticide resistance is likely conferred by ancient trait(s) in T. infestans sylvatic populations, which are capable of invading domiciles. These results emphasize the need for stronger entomological surveillance in the region, including early detection of house invasion, particularly postspraying, monitoring for resistance to pyrethroids and the design of integrative control actions that consider sylvatic foci around domestic settings and their dispersion dynamics.

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“…Domiciliary populations of triatomine bugs have been successfully controlled by spraying of pyrethroid insecticides across large parts of Latin America, contributing to reductions in their distribution from an estimated 6.28 million km 2 in the 1960s to less than 1 million km 2 today [10]. Despite these achievements, the success of contemporary Chagas disease vector control programmes is threatened by persistent peri-domestic foci [11,12], the emergence of insecticide resistance [13][14][15] and household invasion from sylvatic triatomine bug populations [16][17][18][19].…”

Section: Author Summarymentioning

confidence: 99%

Towards environmental detection of Chagas disease vectors and pathogen

Gysin

Urbano

Brandner-Garrod

et al. 2021

Preprint

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Background: Accurate surveillance of triatomine household infestation is crucial for Chagas disease vector control. However, no gold standard detection method with high levels of sensitivity or specificity is currently available. Several intrinsic features of triatomine bug behaviour and the lifecycle of Trypanosoma (T.) cruzi lead to deposition of environmental DNA (eDNA) in infested houses. This study evaluated the use of FTA cards and cotton-tipped swabs as low-technology, cost-effective tools for simultaneous detection of T. cruzi and vector eDNA in the laboratory and field. Methods/Principal Findings: This study had two components: (1) laboratory evaluation and optimisation of QIAcard® FTA® classic cards to detect Rhodnius (R.) prolixus eDNA by altering five different environmental variables (darkness, triatomine number, temperature, feeding status and degradation at ambient temperature); (2) detection of R. prolixus and T. cruzi eDNA from cotton-tipped house wall swabs from an endemic region in Casanare Department, Colombia. eDNA was extracted from all specimens and amplified using a multiplex TaqMan qPCR assay targeting the R. prolixus 12S rRNA gene and T. cruzi satellite DNA region. R. prolixus eDNA from five 3rd/4th instar nymphs was successfully amplified from FTA cards after as little as 15 minutes of contact time under standard insectary conditions. Factors significantly increasing eDNA detection from FTA cards were increasing temperature from 21oC to 27-32oC, triatomine bug density from 1-25 bugs and recent blood-feeding. eDNA was detectable from FTA cards stored at room temperature for at least two weeks. In cotton-tipped swabs from the field, the sensitivity and specificity of R. prolixus eDNA detection was 60.6% (n=20/33) and 100% (n=33/33), respectively. T. cruzi eDNA was amplified from 93.9% (n=31/33) of infested houses. Conclusions/Significance: FTA cards are a highly sensitive tool for entomological surveillance of R. prolixus and exhibit little variability under different environmental conditions. Additionally, cotton-tipped swabs are a relatively sensitive tool for entomological and parasitological surveillance of R. prolixus and T. cruzi in situ, but more feasible due to low cost. Both methods could be utilised by citizen science initiatives to contribute to the control of Chagas disease in endemic communities.

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Genetic structure of deltamethrin-resistant populations of Triatoma infestans (Hemiptera: Reduviidae) in the Gran Chaco

Cited by 10 publications

References 51 publications

Transcriptomic modulation in response to an intoxication with deltamethrin in Triatoma infestans, vector of Chagas disease

Transcriptomic modulation in response to an intoxication with deltamethrin in Triatoma infestans, vector of Chagas disease

Insights into the evolution and dispersion of pyrethroid resistance among sylvatic Andean Triatoma infestans from Bolivia

Towards environmental detection of Chagas disease vectors and pathogen

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