Genetic structure of Galdieria populations from Iceland

Eren, Ayla; Iovinella, Manuela; Yoon, Hwan Su; Cennamo, Paola; Stefano, Mario De; Castro, Olga De; Ciniglia, Claudia

doi:10.1007/s00300-018-2308-3

Cited by 18 publications

(19 citation statements)

References 30 publications

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“…inImamura et al 2010). By phylogenetic analysis, G. maxima is more strictly related to C. merolae than to G. sulphuraria, despite the striking morphological divergences among the two genera[9,12,13]. It is intriguing that G. maxima is provided by a nitrate assimilation gene toolkit similar to that of C. merolae, thus supporting their phylogenetic relationships (H.S.…”

mentioning

confidence: 81%

“…Eight Galdieria maxima strains, supplied from the Algal Collection of University Federico II of Naples (www.acuf.net) and formerly collected from Turkey [13], Iceland [9,12], and Russia [16], were selected based on the pH of the original collection site (Table 1). All strains were isolated by streaking across the agar plates and colonies were inoculated in Allen medium containing (NH4)2SO4 as the nitrogen source, at pH 1.5 [26] and cultivated at 42º C under continuous fluorescent illumination of 50µmol photons• m -2 • sec -1 until they reached the exponential phase.…”

Section: Strains Cultivationmentioning

confidence: 99%

“…We retrieved from GenBank 197 rbcL nucleotide sequences from different Cyanidiophyceae species and strains [6][7][8][9]12,13]; G. maxima ACUF551 was lacking in the database, so DNA extraction was performed, using the protocol described in Ciniglia et al, 2015 [27]. Four degenerate primers were used to amplify the rbcL gene from the sample [6].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

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Prevalent pH Controls the Capacity of <em>Galdieria maxima</em> to Use Ammonia and Nitrate as a Nitrogen Source

Iovinella

Carbone

Cioppa

et al. 2019

Preprint

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Galdieria maxima is a polyextremophilic alga capable of diverse metabolic processes. Ammonia is widely used in culture media typical of laboratory growth. Recent reports that this species can grow on wastes promote the concept that G. maxima might have biotechnological utility. Accordingly, there is a need to know the range of pH levels that can support G. maxima growth in a given nitrogen source. Here, we examined the combined effect of pH and nitrate/ammonium source on the growth and long-term response of the photochemical process to a pH gradient in different G. maxima strains. All were able to use differing nitrogen sources, despite both the growth rate and photochemical activity were significantly affected by the combination with the pH. All strains acidified the NH4+-medium (pH<3); only G. maxima IPPAS P507 showed reduced capacity in lowering the pH from 6.5. pH was a limiting factor in nitrate uptake at pH≥6.5; noteworthy, at pH 5 on nitrate G. maxima ACUF551 showed a good growth performance, despite the alkalization of the medium.

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confidence: 81%

Section: Strains Cultivationmentioning

confidence: 99%

Section: Phylogenetic Analysismentioning

confidence: 99%

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Prevalent pH Controls the Capacity of <em>Galdieria maxima</em> to Use Ammonia and Nitrate as a Nitrogen Source

Iovinella

Carbone

Cioppa

et al. 2019

Preprint

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“…sulphuraria generally grows at low light intensities in the natural environment (Pinto et al 2007;Eren et al 2018) and also showed promising results in both liquid and immobilized cultivation systems with respect to its physiology and in relation to applications in biotechnology .(e.g. Sano et al 2001;Oesterhelt et al 2007;Carbone et al 2020).…”

Section: Figurementioning

confidence: 99%

Comparison of Galdieria Growth and Photosynthetic Activity in Different Culture Systems

Carbone

Olivieri

Pollio

et al. 2020

Preprint

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In the last years, the acidothermophilic red microalga Galdieria sulphuraria has been increasingly studied for industrial applications such as wastewater treatment, recovery of rare earth elements, production of phycobilins. However, even now it is not possible an industrial cultivation of this organism because biotechnological research on G. sulphuraria and allied species is relatively recent and fragmented. Having in mind a possible scale-up for commercial applications, we have compared the growth and photosynthetic performance of G. sulphuraria in four suspended systems (Inclined bubble column, Decanter Laboratory Flask, Tubular Bioreactor, Ultra-flat plate bioreactor) and one immobilized system (Twin Layer Sytem). The results showed that G. sulphuraria had the highest growth, productivity and photosynthetic performance, when grown on the immobilized system, which also offers some economics advantages.

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“…The genus Cyanidium includes two species, both having a key role in the composition of biofilms occurring in extreme environments: C. caldarium (Tilden) Geitler is a polyextremophilic taxon adapted to acidic (pH 0.5-3.0) and thermal (35-55 C) soils, retrieved from hot springs and fumaroles, worldwide (Ciniglia et al 2014;Eren et al 2018;Iovinella et al 2018); C. chilense, formerly 'cave Cyanidium' (Hoffmann 1994), is a neutrophilic (pH around 7.0) and mesophilic (20-25 C) strain isolated by several authors from caves, considered as extreme environments, since nutrient input, light intensity, temperature, and humidity are limiting factors for microorganisms (Pedersen 2000).…”

Section: Introductionmentioning

confidence: 99%

Cyanidium chilense (Cyanidiophyceae, Rhodophyta) from tuff rocks of the archeological site of Cuma, Italy

Ciniglia

Cennamo

Natale

et al. 2019

Phycological Research

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SUMMARY Phlegrean Fields is a large volcanic area situated southwest of Naples (Italy), including both cave and thermoacidic habitats. These extreme environments host the genus Cyanidium; the species C. chilense represents a common phototrophic microorganism living in anthropogenic caves. With a view to provide a comprehensive characterization for a correct taxonomic classification, morpho‐ultrastructural investigations of C. chilense from Sybil's cave (Phlegren Fields) was herein carried out and compared with the thermoacidophilic C. caldarium. The biofilm was also analyzed to define the role of C. chilense in the establishment of a biofilm within cave environments. Despite the peculiar ecological and molecular divergences, C. chilense and C. caldarium shared all the main diacritic features, suggesting morphological convergence within the genus; cytological identity was found among C. chilense strains geographically distant and adapted to different substrates, such as the porous yellow tuff of Sybil cave, and calcyte, magnesite and basaltic rocks from other caves. C. chilense is generally dominant in all biofilms, developing monospecific islets, developing both superficially or between fungal hyphae and coccoid cyanobacteria. Extracellular polymeric substances (EPS) were recorded in C. chilense biofilms from Sybil cave, confirming the role of EPS in facilitating cells adhesion to the surface, creating a cohesive network of interconnecting biofilm cells.

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Genetic structure of Galdieria populations from Iceland

Cited by 18 publications

References 30 publications

Prevalent pH Controls the Capacity of <em>Galdieria maxima</em> to Use Ammonia and Nitrate as a Nitrogen Source

Prevalent pH Controls the Capacity of <em>Galdieria maxima</em> to Use Ammonia and Nitrate as a Nitrogen Source

Comparison of Galdieria Growth and Photosynthetic Activity in Different Culture Systems

Cyanidium chilense (Cyanidiophyceae, Rhodophyta) from tuff rocks of the archeological site of Cuma, Italy

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