Genetic Structure of<i>Plasmodiophora brassicae</i>Populations Virulent on Clubroot Resistant Canola (<i>Brassica napus</i>)

Askarian, Homa; Akhavan, Alireza; Galindo‐González, Leonardo; Hwang, Sheau‐Fang; Strelkov, Stephen E.

doi:10.1094/pdis-09-20-1980-re

Cited by 6 publications

(5 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thirty-eight P. brassicae single-spore isolates were included in this study. The isolation of purified genomic DNA from resting spores used in our study was previously reported [ 33 ]. The DNA was quantified with a Qubit 2.0 DNA HS Assay (Thermo Scientific, Waltham, MA, USA) and DNA quality was assessed with a TapeStation Genomic DNA Assay (Agilent Technologies, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Clubroot of canola (Brassica napus), caused by the soilborne pathogen Plasmodiophora brassicae, has become a serious threat to canola production in Canada. The deployment of clubroot-resistant (CR) cultivars is the most commonly used management strategy; however, the widespread cultivation of CR canola has resulted in the emergence of new pathotypes of P. brassicae capable of overcoming resistance. Several host differential sets have been reported for pathotype identification, but such testing is time-consuming, labor-intensive, and based on phenotypic classifications. The development of rapid and objective methods that allow for efficient, cost-effective and convenient pathotyping would enable testing of a much larger number of samples in shorter times. The aim of this study was to develop two pathotyping assays, an RNase H2-dependent PCR (rhPCR) assay and a SNaPshot assay, which could quickly differentiate P. brassicae pathotypes. Results Both assays clearly distinguished between pathotype clusters in a collection of 38 single-spore isolates of P. brassicae. Additional isolates pathotyped from clubbed roots and samples from blind testing also were correctly clustered. The rhPCR assay generated clearly differentiating electrophoretic bands without non-specific amplification. The SNaPshot assay was able to detect down to a 10% relative allelic proportion in a 10:90 template mixture with both single-spore isolates and field isolates when evaluated in a relative abundance test. Conclusions This study describes the development of two rapid and sensitive technologies for P. brassicae pathotyping. The high-throughput potential and accuracy of both assays makes them promising as SNP-based pathotype identification tools for clubroot diagnostics. rhPCR is a highly sensitive approach that can be optimized into a quantitative assay, while the main advantages of SNaPshot are its ability to multiplex samples and alleles in a single reaction and the detection of up to four allelic variants per target site.

show abstract

Section: Methodsmentioning

confidence: 99%

Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Finally, PI dye solution was added to stain the nuclei, and the cells were placed on ice for 30.0 min. The nuclei were visualized by a microscope [ 24 , 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

Germplasm Enhancement and Identification of Loci Conferring Resistance against Plasmodiophora brassicae in Broccoli

Xie

Wei

Liu

et al. 2022

Genes

View full text Add to dashboard Cite

In order to breed broccoli and other Brassica materials to be highly resistant to clubroot disease, 41 Brassicaceae varieties were developed and identified between 2020 and 2021. Seven known clubroot genes were used for screening these materials. In addition, the resistant and susceptible broccoli cultivars were designed for observing their differences in the infection process with Plasmodiophora brassicae. The results showed that 90% of total materials had carried more than two clubroot resistance genes: one material carried two disease resistance genes, four materials carried seven genes for clubroot resistance, two materials carried six genes for clubroot resistance, and in total 32% of these materials carried five genes for clubroot resistance. As a result, several new genotypes of Brassicaceae germplasm were firstly created and obtained based on distant hybridization and identification of loci conferring resistance against Plasmodiophora brassicae in this study. We found and revealed that similar infection models of Plasmodiophora brassicae occurred in susceptible and resistant cultivars of broccoli, but differences in infection efficiency of Plasmodiophora brassicae also existed in both materials. For resistant broccoli plants, a small number of conidia formed in the root hair, and only a few spores could enter the cortex without forming sporangia while sporangia could form in susceptible plants. Our study could provide critical Brassica materials for breeding resistant varieties and new insight into understanding the mechanism of plant resistance.

show abstract

“…In Fuling, China, clubroot is one of the main diseases in the production of tumorous stem mustard, which is the main factor threatening the development of the pickle industry. P. brassicae is an obligate parasite soil-borne plant pathogen, that causes root galls or clubs, resulting in water and nutrient absorption disorders, causing foliar wilting and even plant death, and ultimately, major yield losses in crop plants ( Askarian et al, 2021 ). Management of clubroot is mainly through resistant varieties and chemical fungicides ( Peng et al, 2014 ; Niemann et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Magnesium oxide nanoparticles reduce clubroot by regulating plant defense response and rhizosphere microbial community of tumorous stem mustard (Brassica juncea var. tumida)

Liao,

Yuan,

Wang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Clubroot, caused by Plasmodiophora brassicae, is a major disease that significantly impairs the yield of cruciferous crops and causes significant economic losses across the globe. The prevention of clubroot, especially in tumorous stem mustard (without resistant varieties), are is limited and primarily relies on fungicides. Engineered nanoparticles have opened up new avenues for the management of plant diseases, but there is no report on their application in the prevention of clubroot. The results showed that the control efficacy of 500 mg/L MgO NPs against clubroot was 54.92%. However, when the concentration was increased to 1,500 and 2,500 mg/L, there was no significant change in the control effect. Compared with CK, the average fresh and dry weight of the aerial part of plants treated with MgO NPs increased by 392.83 and 240.81%, respectively. Compared with the F1000 treatment, increases were observed in the content of soil available phosphorus (+16.72%), potassium (+9.82%), exchangeable magnesium (+24.20%), and water-soluble magnesium (+20.64%) in the 1,500 mg/L MgO NPs treatment. The enzyme-linked immune sorbent assay (ELISA) results showed that the application of MgO NPs significantly increased soil peroxidase (POD, +52.69%), alkaline protease (AP, +41.21%), alkaline phosphatase (ALP, +79.26%), urease (+52.69%), and sucrase (+56.88%) activities; And also increased plant L-phenylalanine ammonla-lyase (PAL, +70.49%), polyphenol oxidase (PPO, +36.77%), POD (+38.30%), guaiacol peroxidase (POX, +55.46%) activities and salicylic acid (SA, +59.86%) content. However, soil and plant catalase (CAT, −27.22 and − 19.89%, respectively), and plant super oxidase dismutase (SOD, −36.33%) activities were significantly decreased after the application of MgO NPs. The metagenomic sequencing analysis showed that the MgO NPs treatments significantly improved the α-diversity of the rhizosphere soil microbial community. The relative abundance of beneficial bacteria genera in the rhizosphere soil, including Pseudomonas, Sphingopyxis, Acidovorax, Variovorax, and Bosea, was significantly increased. Soil metabolic functions, such as oxidative phosphorylation (ko00190), carbon fixation pathways in prokaryotes (ko00720), indole alkaloid biosynthesis (ko00901), and biosynthesis of various antibiotics (ko00998) were significantly enriched. These results suggested that MgO NPs might control clubroot by promoting the transformation and utilization of soil nutrients, stimulating plant defense responses, and enriching soil beneficial bacteria.

show abstract

Genetic Structure ofPlasmodiophora brassicaePopulations Virulent on Clubroot Resistant Canola (Brassica napus)

Cited by 6 publications

References 57 publications

Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

Germplasm Enhancement and Identification of Loci Conferring Resistance against Plasmodiophora brassicae in Broccoli

Magnesium oxide nanoparticles reduce clubroot by regulating plant defense response and rhizosphere microbial community of tumorous stem mustard (Brassica juncea var. tumida)

Contact Info

Product

Resources

About