Genetic structure of invasive ctenophore Mnemiopsis leidyi populations in temperate northern European waters supports the southern North Sea overwintering refuge

Abstract

The ctenophore Mnemiopsis leidyi is native to the Atlantic coasts of the Americas and is considered a threat to biodiversity and food webs in introduced ecosystems. Most of these invasive ctenophores perish during the cold winter in temperate northern European waters (North and Baltic Seas), but spread again every summer/autumn. We collected ctenophores from the southern North Sea, inner Danish waters, and western Baltic Sea in 2017 and 2018, and sequenced genomic DNA obtained from tissue samples. We obtained sequences of internal transcribed spacer (ITS) 1 and 2, and the intervening 5.8S fragment and partial fragments of the flanking 18S rRNA and 28S rRNA genes from nuclear DNA (nDNA). These sequences were compared with ones archived in databases from specimens in its native (Northwest Atlantic) and other invaded habitats (Caspian Sea, Mediterranean Sea, central-western Atlantic Ocean). The comparison revealed no significant difference in genetic internal variation among temperate northern European sampling locations but showed variation when compared with native and other invaded habitats. No significant genetic difference was identified between specimens collected in the temperate northern European waters in the two consecutive years 2017 and 2018. The very low interregional and interannual genetic variations in M. leidyi specimens in the Northeast Atlantic indicate that its distribution in temperate northern European waters can be regarded as one single panmictic population and that the annual dispersal from the southeastern North Sea into the western Baltic Sea can be traced back to the same population, which most likely originates every spring from the Dutch Wadden Sea and the English Channel.