Genetic structure of Japanese populations of an ambrosia beetle, <i>Xylosandrus germanus</i> (Curculionidae: Scolytinae)

Ito, Mizuho; Kajimura, Hisashi; Hamaguchi, Keiko; Araya, Kunio; Lakatos, Ferenc

doi:10.1111/j.1479-8298.2008.00280.x

Cited by 16 publications

(22 citation statements)

References 43 publications

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“…1). These values are similar to those calculated as differences among some species in the genus Tomicus (Dϭ0.104-0.141; Duan et al 2004) and Ips (DϾ0.120; Lakatos et al, 2007) of scolytine bark beetles; however, in other species of Scolytinae, large values of genetic distance were found among populations (e.g., X. germanus, DϷ0.112-0.150;Ito et al, 2008;Ozopemon brownei Hagedorn, DϷ0.073-0.130, Jordal et al, 2002a; Coccotrypes fallax (Eggers), DϷ0.095, Jordal et al, 2002b). Experiments such as comparison of the male genital morphology and crossbreeding between insects with different mtDNA clades are needed to clarify whether X. crassiusculus forms a species complex.…”

Section: Discussionsupporting

confidence: 62%

“…The genetic structures of a leaf beetle, Chrysolina virgata (Motschusky) (Sota et al, 2004) and an ambrosia beetle, X. germanus (Ito et al, 2008) suggest that distinct mtDNA lineages represent colonizers of different epochs in Japan. The mtDNA subclades of X. crassiusculus may also have colonized the islands independently.…”

Section: Discussionmentioning

confidence: 99%

“…800 bp mtDNA that included a portion of the cytochrome oxidase I gene (COI) by polymerase chain reaction (PCR), using primers C1-J-2183 (5Ј-CAA CAT TTA TTT TGA TTT TTT GG-3Ј; Simon et al, 1994) and TL-2-N-3014-ANT (5Ј-TGA AGT TTA AGT TCA ATG CAC-3Ј; slightly modified from the primer used by Simon et al, 1994; K. Hamaguchi, unpublished data; see also Ito et al, 2008). For PCR analysis, we mixed 1 ml extracted DNA, 2 ml each primer (5 pmol/ml), 0.8 ml dNTPs (Takara, Otsu City, Shiga, Japan), 1 ml 10ϫ PCR buffer (Takara), 0.1 ml Taq DNA polymerase (5 units/ml, Takara), and 3 ml distilled water in a total volume of 10 ml.…”

Section: Insectsmentioning

confidence: 99%

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Phylogeography of an ambrosia beetle, Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae: Scolytinae), in Japan

Ito

Kajimura

2009

Appl. Entomol. Zool.

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We examined the genetic structure of populations of an ambrosia beetle, Xylosandrus crassiusculus. Specimens were collected from 22 sites in Japan and their genetic structure was studied using a portion of the mitochondrial cytochrome oxidase I gene. Phylogenetic analysis revealed three distinct lineages (clades A, B and C), each of which was divided into two subclades (A 1 , A 2 , B 1 , B 2 , C 1 and C 2 ). Subclade A 1 had 17 haplotypes from Hokkaido, Honshu and Kyushu populations, subclade A 2 5 haplotypes mainly from Shikoku populations, subclade B 1 15 haplotypes from central and western Honshu and Amami populations, subclade B 2 2 haplotypes only from Naha population, subclade C 1 4 haplotypes from Amami and Naha populations, and subclade C 2 5 haplotypes only from Ishigaki population. Analysis of molecular variance detected genetic differentiation among populations. The data imply that the genetic structure of Japanese populations of X. crassiusculus has been affected by the geological history of the Japanese islands, but that the mtDNA phylogeographic pattern is so complex that we could not infer any simple scenario of population history in X. crassiusculus.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Insectsmentioning

confidence: 99%

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Phylogeography of an ambrosia beetle, Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae: Scolytinae), in Japan

Ito

Kajimura

2009

Appl. Entomol. Zool.

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“…Amplifications were performed with standard PCR techniques. The reaction mixture and thermal cycling conditions followed the protocol of Ito et al (2008). The PCR product was purified using a QIAquick PCR Purification Kit (Qiagen Inc., Valencia, California, USA).…”

Section: Mitochondrial Dna Analysismentioning

confidence: 99%

Differential butterfly performance on host plant variants from populations under intense vs. low mammalian herbivory

et al. 2017

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Abstract. Evolutionary interactions between a pair of species can be modified by the presence of another species that interacts with either or both species. However, only a few studies have demonstrated such complex interactions. Here, we report a case where a population of the red admiral butterfly (Vanessa indica) is assumed to change fitness traits in response to an evolutionary change in its major host plant, the Japanese stinging nettle (Urtica thunbergiana), caused by intense browsing pressure from sika deer (Cervus nippon). Nara Park (NP) in Japan has been home to several hundred protected sika deer for approximately 1200 yr, and their intense browsing pressure is assumed to have selected for heavily haired nettles that are more resistant to deer browsing compared to normal lightly haired nettles occurring in areas with low densities of sika deer. We found that the water and nitrogen contents of leaves are lower and the specific leaf mass is higher in heavily haired nettles at NP compared to the lightly haired nettles at the Takatori Castle Site (TCS) 30 km south of NP. Feeding experiments showed that V. indica larvae from NP reared on heavily haired nettles reach a greater adult body mass and relative abdomen mass than those from TCS, suggesting the possibility that the NP V. indica population has adapted to the heavily haired, nutritionally poorer variant. Analysis of the nucleotide sequence of the mitochondrial cytochrome c oxidase subunit I gene of V. indica from NP and nearby locations including TCS found one haplotype exclusive to NP, suggesting that movement of this butterfly is somewhat restricted. However, analysis using amplified fragment length polymorphism markers showed no genetic differentiation between the seven V. indica populations including the NP and TCS populations. These results suggest that adaptation of the NP population to the heavily haired nettle occurred in a relatively short period. Our study demonstrates a rare example of an indirect evolutionary impact of one herbivore (sika deer) on another herbivore (red admiral butterfly) through an evolutionary change in their shared food plant (Japanese nettle).

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“…A phylogenetic analysis based on cytochrome oxidase I ( COI ) mitochondrial DNA (mtDNA) revealed three distinct lineages (clades A, B, and C) within X. germanus in Japan (Ito, Kajimura, Hamaguchi, Araya, & Lakatos, ). The rates of substitutions per site between the three lineages are 12.4%–15.0%, which are similar to those calculated as differences among scolytine beetle species in the genera Ips , Tomicus , and Dendroctonus (Cai, Cheng, Xu, Duan, & Kirkendall, ; Cognato & Sperling, ; Duan, Kerdelhué, & Lieutier, ; Lakatos, Grodzki, Zhang, & Stauffer, ; Maroja, Bogdanowicz, Wallin, Raffa, & Harrison, ).…”

Section: Introductionmentioning

confidence: 99%

Landscape‐scale genetic differentiation of a mycangial fungus associated with the ambrosia beetle,Xylosandrus germanus(Blandford) (Curculionidae:Scolytinae) in Japan

Ito

Kajimura

2017

Ecology and Evolution

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In this study, we examined the genetic structures of the ambrosia fungus isolated from mycangia of the scolytine beetle, Xylosandrus germanus to understand their co‐evolutionary relationships. We analyzed datasets of three ambrosia fungus loci (18S rDNA, 28S rDNA, and the β‐tubulin gene) and a X. germanus locus dataset (cytochrome c oxidase subunit 1 (COI) mitochondrial DNA). The ambrosia fungi were separated into three cultural morphptypes, and their haplotypes were distinguished by phylogenetic analysis on the basis of the three loci. The COI phylogenetic analysis revealed three distinct genetic lineages (clades A, B, and C) within X. germanus, each of which corresponded to specific ambrosia fungus cultural morphptypes. The fungal symbiont phylogeny was not concordant with that of the beetle. Our results suggest that X. germanus may be unable to exchange its mycangial fungi, but extraordinary horizontal transmission of symbiotic fungi between the beetle's lineages occurred at least once during the evolutionary history of this symbiosis.

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Genetic structure of Japanese populations of an ambrosia beetle, Xylosandrus germanus (Curculionidae: Scolytinae)

Cited by 16 publications

References 43 publications

Phylogeography of an ambrosia beetle, Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae: Scolytinae), in Japan

Phylogeography of an ambrosia beetle, Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae: Scolytinae), in Japan

Differential butterfly performance on host plant variants from populations under intense vs. low mammalian herbivory

Landscape‐scale genetic differentiation of a mycangial fungus associated with the ambrosia beetle,Xylosandrus germanus(Blandford) (Curculionidae:Scolytinae) in Japan

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