Genetic structure of Mount Huang honey bee (Apis cerana) populations: evidence from microsatellite polymorphism

Liu, Fang; Shi, Tengfei; Huang, Sisi; Yu, Linsheng; Bi, Shoudong

doi:10.1186/s41065-016-0010-4

Cited by 11 publications

(3 citation statements)

References 12 publications

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“…Under the complex ecological conditions in China, Chinese honeybees are well adapted to flowering plants and climatic variation in different regions, thus playing an irreplaceable and extremely important ecological role relative to that of exotic bees (Zhou et al, 2021;Chen and Luo, 2023). Research has shown that even Chinese honeybee populations in limited geographic areas may have great genetic differences, resulting in high diversity and providing a material foundation for the development and utilization of Chinese honeybee genetic resources (Yin and Ji, 2013;Liu et al, 2016;Yu et al, 2019;Cao et al, 2021;Fang et al, 2022). Changes in the climate and environment, industrialization, pesticide implementation, and other factors have affected honeybee resources.…”

Section: Introductionmentioning

confidence: 99%

Whole-genome resequencing reveals genetic diversity and adaptive evolution in Chinese honeybee (Apis cerana cerana) in Guizhou, China

Wang,

Zeng,

Deng

et al. 2024

Front. Genet.

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Introduction: Guizhou Province, characterized by complex and diverse geographic and climatic environments, has rich genetic resources for the Chinese honeybee (Apis cerana cerana) and is one of the main bee-producing areas in China. However, research on the genetic diversity of Chinese honeybee in the Guizhou region is very limited, despite implications for conservation of biodiversity.Methods: In this study, we analyzed the genetic diversity, differentiation, and selection signals based on 116 Chinese honeybees from 12 regions in Guizhou Province using whole-genome sequencing.Results: We identified 1,400,430 high-quality SNPs across all samples. A population structure analysis revealed two independent genetic subgroups of Chinese honeybees in Guizhou, a Yunnan-Guizhou Plateau population in western Guizhou and a hilly-mountainous population in eastern Guizhou. The average nucleotide diversity (Pi) ranged from 0.00138 to 0.00161 and average expected heterozygosity (He) ranged from 0.2592 to 0.2604. The average genetic differentiation index (FST) for Chinese honeybees in pairwise comparisons of 12 regions ranged from 0.0094 to 0.0293. There was clear genetic differentiation between the western plateau and the eastern hilly mountainous areas of Guizhou; however, FST values between the eastern and western populations ranged from 0.0170 to 0.0293, indicating a low degree of differentiation. A genome-wide scan revealed a number of genes under selection in the Yunnan-Guizhou Plateau environment. These genes were related to growth and development, reproduction, and cold resistance, and several candidate genes involved in environmental adaptation were identified, including CTR, MAPK, MAST, HSF, and MKKK.Discussion: The results of the present study provide important theoretical bases for the conservation, evaluation, development, and utilization of genetic resources for Chinese honeybees in the Guizhou region and for further investigations of environmental adaptation and underlying mechanisms in the species.

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Section: Introductionmentioning

confidence: 99%

Whole-genome resequencing reveals genetic diversity and adaptive evolution in Chinese honeybee (Apis cerana cerana) in Guizhou, China

Wang,

Zeng,

Deng

et al. 2024

Front. Genet.

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“…Nevertheless, Sheppard and Meixner [7] consider the honeybee subspecies Apis mellifera pomonella to be endemic to Kazakhstan. This subspecies is believed the result of the crossbreeding between Apis mellifera and Apis cerana [8].…”

Section: Introductionmentioning

confidence: 99%

Genetic investigation of honeybee populations in Kazakhstan

Gritsenko

Temirbayeva

Taskuzhina

et al. 2023

Preprint

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Beekeeping as a staple of agriculture in Kazakhstan is believed to have emerged when external bee specimens were introduced into the country. The Central Russian bee (Apis mellifera mellifera) has been present throughout Kazakhstanian apiaries for a long time since its import into Eastern Kazakhstan at the end of the 18th century. To date, six subspecies have been distributed across the country (A.m. sossimai, A.m. carpatica, A.m. mellifera, A.m. ligustica, A.m. caucasica, and A.m. carnica). According to mitochondrial haplotype analysis based on DraI mtDNA COI-COII (DmCC) test, local samples were represented by C2 (316), C1 (99), and M4 (7) haplotypes. The results of simple sequence repeats (SSR) genotyping revealed a large polymorphism at nine microsatellite loci, with the number of alleles amounting to 35 (AP55), 32 (AP43), 25 (A124), 18 (A113), 13 (A88), 12 (A43), 11 (A007), 7 (A28), and 5 (A24). Relative to the expected heterozygosity (He), the observed heterozygosity (Ho) was slightly higher for most markers considering both the overall samples and individual populations. The inbreeding coefficient confirmed the excess outbreeding for the geographical populations of car-Shym-T (-0,105), car-zham-A (-0,114), Zhet-Alakol (-0,008), and Zhet-Ushbulak (-0,028). The admixture of honeybee local populations was confirmed by the research presented here.The differentiation of populations was only possible by geographical location according to clustering analysis. A considerable degree of genetic admixtures among subspecies was identified in every population. The subspecies were not separated from each other. None of the groups formed by the neighbor-joining tree based on Nei's genetic distance included a precise subspecies or population. All groups represented an admixture of subspecies from different populations. Unregulated cross-breeding for the past 50 years has laid the foundation for the promiscuous genetic nature of honeybee populations in Kazakhstan. It could be concluded that some samples were the result of cross-breeding with endemic bee Apis mellifera pomonella since most apiaries were located in areas of endemic bee distribution.

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“…Many molecular markers such as restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and mitochondrial DNA (mtDNA) analyses have been used in honey bee population genetics studies, but for the last 20 years, microsatellite loci have been quite widely used due to their features such as high polymorphism, multiallelicity, abundance in the genome and easy scorable (Kandemir & Kence, 1995;Smith et al, 1997;Bodur et al, 2007;Kekeçoğlu et al, 2009;Özdil et al, 2009;Liu et al, 2016;Rahimi et al, 2016;Haddad et al, 2018;Hassett et al, 2018;Yu et al, 2019;Özdil et al, 2022). Latterly, single nucleotide polymorphisms (SNPs) have been used in population genetics studies, however, thanks to the advantages provided by microsatellites, they could not get ahead of microsatellites even in the genomic era (Zimmerman et al, 2020;Mukherjee et al, 2022;Wang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

İzole bölgelerde korunan Kafkas arısı, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) popülasyonunda genetik varyasyonun azalıp azalmadığına dair bir çalışma

YILDIZ,

TÜTEN,

AYDIN

et al. 2023

TED

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The Caucasian honeybee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae), is one of the most productive bee subspecies. This subspecies, which has special importance for Türkiye, has been taken under protection in two isolated regions (Artvin and Ardahan) since 2000. To date, no study has been conducted on whether genetic diversity has decreased in these protected Caucasian honeybee colonies. Therefore in 2022, worker bees were collected from 100 different colonies in 15 different locations in these two regions and their genetic variations were examined using 30 microsatellite loci. The average number of alleles per locus was 13.57, and the loci had a high level of information content according to the PIC (0.7) value. FIS (0.96) and FST (0.01) values showed low genetic diversity and high inbreeding in populations. Genetic variations were calculated as 0.77% among populations, 99.23% among individuals in populations, and 0% among all individuals. Also, populations deviated from the Hardy-Weinberg equilibrium (p<0.001). Significant bottleneck evidence was found for Artvin in the analysis results using the two-phase mutation model. These results provide important information that can be used as a guide for Caucasian bee breeding strategies and conservation programs.

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Genetic structure of Mount Huang honey bee (Apis cerana) populations: evidence from microsatellite polymorphism

Cited by 11 publications

References 12 publications

Whole-genome resequencing reveals genetic diversity and adaptive evolution in Chinese honeybee (Apis cerana cerana) in Guizhou, China

Whole-genome resequencing reveals genetic diversity and adaptive evolution in Chinese honeybee (Apis cerana cerana) in Guizhou, China

Genetic investigation of honeybee populations in Kazakhstan

İzole bölgelerde korunan Kafkas arısı, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) popülasyonunda genetik varyasyonun azalıp azalmadığına dair bir çalışma

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