Genetic structure of phenotypic variants and somaclones of the genus<i>Cereus</i>(Cactaceae) as revealed by AFLP markers

Eloi, I. B. O.; Lucena, Ana Luisa Monezi; Mangolin, Claudete Aparecida; Machado, M. F. P. S.

doi:10.1080/14620316.2016.1276864

Cited by 7 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such technology is reliable, cheap and could be used to detect genetic variability on large scale. The amplified fragment length polymorphism (AFLP), SCoT, SRAP, and DNA barcoding are the most known molecular marker assays that have been used in studying soma clonal varaitaion in medical plants [10,11]. SRAP marker assay is an easy and effective marker system with many advantages over other systems: flexibility, acceptable throughput, revealing multiple co-dominant markers and tracking open reading frames [12].…”

Section: Introductionmentioning

confidence: 99%

DNA-barcoding, SCoT and SRAP Based Somaclonal Variation in Micropropagated Withania somnifera Plantlets

Ismail¹,

Aljuaid²,

Dessoky³

et al. 2022

JAC

View full text Add to dashboard Cite

Ashwagandha (Withania somnifera) is one of the recognized plant species that considered of most traditional natural supplements. Tissue culture is an efficient method as fast and affordable in plant propagation. Few studies have discussed the genetic impact of such method on ashwagandha plant. The aim of this research was to identify the genetic stability of micropropagated plantlets and to assess the impact of in vitro-propagation on somaclonal variability in ashwagandha using start codon-targeted (SCoT), sequence-related amplified polymorphism (SRAP) and DNA-barcoding assays. SCoT marker assay produced a total number of 132 bands with an average of 11 bands per primer, where scorable PCR fragments were generated from all primers. The phylogenetic tree constructed using SCoT binary data, revealed genetic variability among studied plant samples. SRAP primer combinations showed a total of 78 bands by an average of 11.1 bands / combination, in which all combinations produced scored PCR fragments. Over SRAP assay, one specific band was obtained that was present in different ashwagandha micropropagated plant samples compared to the control (mother plant). This PCR fragments were obtained using me1F/em1R primer combination (287 bp). The phylogenetic tree constructed using SRAP data was successful to differentiate between micro-propagated plants and the control. The DNA- barcoding analysis using chloroplast gene RNA polymerasel (rpoCl) gene was used to detect the soma- clonal variation between control and one micro-propagated plant of ashwagandha. The phylogenetic tree constructed using DNA-barcoding sequences was successful to differentiate between the two samples, where control and micropropagated plantlets were grouped in two different groups. This study suggests the valuableness of using SRAP and DNA-barcoding in detecting soma-clonal variation among micropropagated plantlest of ashwagandha.

show abstract

Section: Introductionmentioning

confidence: 99%

DNA-barcoding, SCoT and SRAP Based Somaclonal Variation in Micropropagated Withania somnifera Plantlets

Ismail¹,

Aljuaid²,

Dessoky³

et al. 2022

JAC

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

A Comparative Study between Major Crop (Potato) and Minor Crop (Onion) in Volcanic Highlands of Rwanda

Maniriho¹,

Musabanganji²,

Lebailly³

2022

JAC

View full text Add to dashboard Cite

This paper aims to assess the competition between the priority (major) crops and the non-priority (minor) crops. Competition between crops is defined as the significant major differences between two crops in terms of production costs and their performance. Data were collected using a questionnaire administered to a random sample of 226 small-scale crop producers including 94 onion producers and 132 potato producers. The T-test was conducted to state whether there is a significant difference of mean land sizes, mean crop yields, mean selling prices, and mean net farm incomes between the two groups of crop producers. Results showed non significant difference between the mean land size allocated to onion production and that allocated to potato farming. Results also indicate that onion yield is significantly greater than potato yield, onion selling price is significantly greater than that of potato, and the net income from onion production is far away greater than the income from potato production. It is remarkable that, in some circumstances, the authorities may prioritize and thus propose to farmers the crops that are less competitive considering their price, yield or income, if the current climatic conditions and economic settings are maintained in the medium or long term. Referring to these findings, policy efforts should encourages to the farmers to shift from potato farming to onion production, or simply inclusion of crop diversification via adopting onion may be the best option to maximize the potentials of the selected crops.

show abstract

Communities of endophytic bacteria from Cereus peruvianus Mill. (Cactaceae) plants obtained from seeds and from in vitro-regenerated somaclone

Beraldo-Borrazzo

Polônio

Schoffen

et al. 2021

South African Journal of Botany

View full text Add to dashboard Cite

Genetic structure of phenotypic variants and somaclones of the genusCereus(Cactaceae) as revealed by AFLP markers

Cited by 7 publications

References 29 publications

DNA-barcoding, SCoT and SRAP Based Somaclonal Variation in Micropropagated Withania somnifera Plantlets

DNA-barcoding, SCoT and SRAP Based Somaclonal Variation in Micropropagated Withania somnifera Plantlets

A Comparative Study between Major Crop (Potato) and Minor Crop (Onion) in Volcanic Highlands of Rwanda

Communities of endophytic bacteria from Cereus peruvianus Mill. (Cactaceae) plants obtained from seeds and from in vitro-regenerated somaclone

Contact Info

Product

Resources

About