Genetic structure of the Capoeta aculeata populations inferred from microsatellite DNA loci

Abstract

Abstract. Gandomkar H, Shekarabi SPH, Abdolhay HA, Nazari S, Mehrjan MS. 2020. Genetic structure of the Capoeta aculeata populations inferred from microsatellite DNA loci. Biodiversitas 21: 4565-4570. The present study aimed to investigate the genetic variation of Capoeta aculeata on the basis of DNA microsatellite loci from three rivers (Beshar, Khersan, Maroun) in Kohgiluyeh and Boyer-Ahmad Province in Iran. DNA from fin clips of 120 specimens extracted and was examined with eight microsatellite markers. Genetic differences between the populations were discerned by pairwise comparison based on allelic distribution. The average number of alleles per locus ranged from 4 to 14, while the average observed heterozygosity (Ho) at various loci varied between 0.212 to 0.579, implying that a moderate level of genetic variation. Among three populations, Maroun River population displayed the highest level of variability in terms of heterozygosity. Tests of Hardy-Weinberg showed that the microsatellite loci deviated significantly in the populations. The results indicate that some of the populations were significantly differentiated from one another based on pairwise FST estimates. Genetic distance-based measures supported the clustering of Maroun, Beshar, and Khersan rivers. The neighbor-joining dendrogram topology constructed on the basis of genetic distances among populations supported observed division between the populations. The non-significant differentiation between C. aculeata samples from Beshar and Khersan can be explained by a relative disconnection of these two populations and/or small amounts of gene flow.

Characterization of novel genotyping-by-sequencing (GBS)-based simple sequence repeats (SSRs) and their application for population genomics of Capoeta aculeata (Valenciennes, 1844)

“…Conservation management plans with no prior information of the genetic background could result in disturbance to the genetic structure with adverse effects on the gene pools of wild populations (Khoshkholgh and Nazari, 2020). Until now, the microsatellite loci have not been carefully developed for C. aculeata which has posed a serious obstacle to conservation and management of this species (Gandomkar et al, 2020). The current study detected 16,706 microsatellite markers in C. aculeata genome using MISA, which accounted for 2.12% of the total genome sequence.…”

“…In fact, SSR markers are tremendously effective tools in population genetic studies because they might reveal the distinct population Segments (DPSs) even in ne-scale genetic structure studies. Until now, microsatellite loci have not been reported in this species (Gandomkar et al, 2020).…”

Radiographic anatomy of the heart of fruit bats

“…The speed of the heart rate is related to the delivery of electrical impulses in the heart. The heart of the bat has multiple trabeculae septomarginalis so that the heart rate is high according to the faster delivery of impulses (Rahma et al, 2020). Also, the description of the results of the clock analogy method can be used in analysing the incidence of enlargement in the cardiac chamber simply.…”

“…The heart apex in four-legged terrestrial mammals tend to lead to the ventral part, whereas in bats, that position tends situated in the middle of thorax cavity with anterio-posterior orientation (Rahma et al 2020). This posture can be influenced by the habit of bats hanging upside down so that adaptation occurs to the pericardial attachment, especially the apex to the diaphragm.…”