Genetic Studies of the Mixed Lymphocyte Reaction in H-2 Identical Mice1

Festenstein, H; Sachs, J. A.; Oliver, R.T.D.

doi:10.1159/000392392

Cited by 27 publications

(39 citation statements)

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“…Although it was not possible to establish the individual haplotypes without family segregation studies, it is interesting to note that of the five individuals who were HLA-A1, -B8, and -Dw3, three were also HLA-DRw3. However, both HLA -Dw3 and -DRw3 specificities are among those that are not yet clearly defined Festenstein et al, 1978). HLA-B8, which has the strongest association, is a well-established specificity.…”

Section: Resultsmentioning

confidence: 99%

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HLA antigens in chronic relapsing idiopathic inflammatory polyneuropathy

Adams

Festenstein

Gibson

et al. 1979

Journal of Neurology, Neurosurgery & Psychiatry

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Section: Resultsmentioning

confidence: 99%

“…The HLA-D specificities were identified by the use of homozygous typing cells accredited in the 1975 184 and 1977 Histocompatibility Workshops (Thorsby and Piazza, 1975;Festenstein et al, 1978). Each patient was tested at least twice for eight specificities (HLA-Dwl-Dw8), and at least two Dw3 cells were incorporated in each expenrment.…”

Section: Resultsmentioning

confidence: 99%

HLA antigens in chronic relapsing idiopathic inflammatory polyneuropathy

Adams

Festenstein

Gibson

et al. 1979

Journal of Neurology, Neurosurgery & Psychiatry

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“…In the mouse also, other genetic determinants which are not linked to the H-2-chromosomal region may provoke MLC-reactivity but not graft versus host reactions (23).…”

Section: Discussionmentioning

confidence: 99%

Two Separate Genes Controlling Stimulation in Mixed Lymphocyte Reaction in Man

Dupont

Good

Hansen

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

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The genetic control of strong stimulation in the mixed lymphocyte culture reaction is determined by a separate gene (MLR-S) Recent findings show that the genetic control of stimulation in the mixed lymphocyte culture (MLC) reaction in man is determined by a separate gene (MLR-S) closely linked to the FOUR-locus of the HL-A chromosomal region (1). This gene must be located outside the 1IL-A chromosomal region as indicated by studies of the AILC-reactions within families with recombinations of the major histocompatibility region (MlHlR) between the FOUR-locus and the MILR-S locus (1-8).In this study we present data on three recombinant children with recombination between the FOUR-locus and the MLR-S locus in two families. The MLC data from these families confirms the hypothesis that the MLR-S locus is located outside the HL-A chromosomal region. An analysis of normal material of zero, one, and two iHL-A haplotype different combinations has made it possible to evaluate the MLC results obtained in the HL-A haploidentical, MLR-S identical combinations. This has clarified that more than one gene is responsible for the stimulation in the MLC test. It is concluded, that the MLR-S locus is responsible for a strong allogenie cell stimulation, while one or more genes located within the ilL-A chromosomal region may contribute with a weak Abbreviations: MILC, mixed lymphocyte culture; S1R, stimulation rates; AMiR, major histocompatibility region; SCII), severe (Table 1 and Fig. 1). A total of 479 of these tests represented unrelated IIL-A nonidentical, two-haplotype different combinations, 127 were haploidentical siblings or parent-child combinations, while the remaining 52 tests were performed between HL-A identical siblings. Calculations of the data obtained withiii each of the three groups were based on logratios. The log-value for mean, SD, SE of mean, was determined and the log-ratios were retransformed into simple ratios for further analysis. One family (Minnesota family BR) (

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“…Non-H2 different congenic combinations generally do not result in stimulation (29). However, an increasing number of exceptions to this generalization have been recognized, and specific loci responsible for mixed lymphocyte cultures have been postulated (30,31). One of the exceptions is found in the stimulation of BALB/c (H2d) lymphocytes by DBA/2 (H2d) cells (18,19).…”

Section: Discussionmentioning

confidence: 99%

“…One of the exceptions is found in the stimulation of BALB/c (H2d) lymphocytes by DBA/2 (H2d) cells (18,19). An M-locus that is not linked to H2 is responsible for this stimulation (31) . Differences at the MLC locus alone generated lymphocyte proliferation but not cytotoxic lymphocytes; it was necessary that both MLC locus and major histocompatibility differences be present and that the target cell differ from the lymphocytes at a major histocompatibility locus in order to be killed.…”

Section: Discussionmentioning

confidence: 99%

Enumeration of Activated Thymus-Derived Lymphocytes by the Virus Plaque Assay

Kano

Bloom

Howe

1973

Proc. Natl. Acad. Sci. U.S.A.

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Lymphocytes activated by antigens or mitogens acquire the capacity to replicate viruses, and the number of activated lymphocytes can be estimated by the virus plaque assay. Concanavalin A and pokeweed mitogen produced 33-fold and 17-fold increases in virus plaqueforming cells (V-PFC), respectively, above background, while lipopolysaccharide produced only a 2-to 3-fold increase. T (thymus-derived lymphocyte)-depleted lymphocyte populations, derived from anti-O-treated or nude (athymic) mouse spleens, failed to produce V-PFC after culture with concanavalin A or pokeweed mitogen. The present studies thus demonstrate that the virus plaque assay measures activated T-lymphocytes.A dissociation between the V-PFC response and cell proliferation was previously observed in antigen-stimulated cells cultured in the presence of mitotic inhibitors.In the present studies, while stimulation of CBA (H2k) lymphocytes by DBA/2 (H2d) cells produced high levels of thymidine incorporation, lymphocyte target-cell cytotoxicity, and V-PFC, stimulation of BALB/c (H2d) lymphocytes against DBA/2 (H2d) cells resulted in even higher levels of thymidine incorporation with a virtual absence of cytotoxic lymphocytes or V-PFC. These results indicate that proliferation is not a sufficient condition for permitting lymphocytes either to exert cytotoxicity on target cells or to replicate viruses, and suggest that there may be a correlation between the development of V-PFC and cytotoxic lymphocytes. They are consistent with the view that there are at least two functional subpopulations of T-lymphocytes.

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Genetic Studies of the Mixed Lymphocyte Reaction in H-2 Identical Mice1

Cited by 27 publications

References 0 publications

HLA antigens in chronic relapsing idiopathic inflammatory polyneuropathy

HLA antigens in chronic relapsing idiopathic inflammatory polyneuropathy

Two Separate Genes Controlling Stimulation in Mixed Lymphocyte Reaction in Man

Enumeration of Activated Thymus-Derived Lymphocytes by the Virus Plaque Assay

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