Genetic studies on seed coat permeability and viability in RILs derived from an inter-specific cross of soybean [Glycine max (L.) Merrill]

Kumar, Ashish; Chandra, Subhash; Talukdar, Akshay; Yadav, Raju R.; Saini, Manisha; Poonia, Shatakshi; Lal, S. K.

doi:10.31742/ijgpb.79.1.7

Cited by 7 publications

(10 citation statements)

References 12 publications

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“…The seed coat permeability found to have negative correlation with seed viability, which is in agreement with Nacer et al (2017) and Kumar et al (2019a). The seed size has positive correlation with mechanical damage and seed coat permeability (Mugnisjah et al 1987;Chandra et al 2017) and negative correlation with seed viability (Tiwari and Joshi 1989;Kumar et al 2019a). The larger seeds found to have more seed coat permeability (Chandra et al 2017).…”

Section: Discussionsupporting

confidence: 82%

“…The black seeds show more storability due to activity of some free fatty acids and phenolic compounds (Liu et al 2017). The seed coat permeability found to have negative correlation with seed viability, which is in agreement with Nacer et al (2017) and Kumar et al (2019a). The seed size has positive correlation with mechanical damage and seed coat permeability (Mugnisjah et al 1987;Chandra et al 2017) and negative correlation with seed viability (Tiwari and Joshi 1989;Kumar et al 2019a).…”

Section: Discussionsupporting

confidence: 77%

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Seed longevity studies in wild type, cultivated and inter-specific recombination inbred lines (RILs) of soybean

Chandra¹,

Talukdar²,

Taak³

et al. 2021

Preprint

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Loss of seed viability is a serious hurdle in higher production and ambient seed storage of soybean. Understanding the factors affecting seed viability and identification of soybean genotype(s) with higher viability is critical for higher soybean production. In this study, seeds of 125 soybean genotypes of three different species (Glycine tomentella, Glycine soja and Glycine max) and 25 RILs (Glycine soja × Glycine soja) were tested for germination immediately after harvest followed by one, two and three years of ambient storage. Fresh seeds of all the genotypes recorded 78-99% germination with a mean of 94.02%. However, the mean value of per cent germination of all the genotypes after one, two and three years of ambient storage decreased and found to stand at 79.51%, 52.24% and 29.18%, respectively. Among the genotypes tested, G. tomentella accession registered highest seed storability followed by G. soja, RILs and G. max. After three years of storage, 14 wild type genotypes and 3 RILs maintained >70% germination and identified as ‘good storers’. Genetic divergence studies via k-mean clustering and principal component analysis grouped all genotypes in three cluster/component based on seed size, seed coat permeability and seed viability. Seed viability was significantly and negatively correlated with electrical conductivity. Good storers found to be having slow imbibition rate than poor storers in initial hours of imbibition. The good storing genotypes identified in this study will serve as an elite soybean genetic resource in developing the soybean cultivars with better seed longevity.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 77%

Seed longevity studies in wild type, cultivated and inter-specific recombination inbred lines (RILs) of soybean

Chandra¹,

Talukdar²,

Taak³

et al. 2021

Preprint

Self Cite

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“…During evolution and domestication, the impermeable seed coat became permeable but not without penalty; the permeable seeds tend to lose viability early. Therefore, it is imperative to develop highly viable seeds with permeable seed coat (Kumar et al 2019). In an attempt to understand the genetic control of seed coat impermeability, it was found in two F 2 populations that a major gene controls this trait in two wild type (G. soja) genotypes viz., PI 424079 and PI 366120.…”

Section: Discussionmentioning

confidence: 99%

“…The 204 RILs in F 7 were used to map the genomic regions controlling seed coat impermeability. Freshly harvested seeds of 204 RILs and their parents were air dried to about 10% moisture contents and packed in cloth bags and stored under ambient conditions of Delhi for different periods (1, 2 and 3 years) with an average relative humidity of 65 ± 5% and temperature of 25 ± 2°C as described by Kumar et al (2019).The results obtained on the RILs were validated in a F 2 population derived from a cross involving seed-coat impermeable genotype PI 424079 with permeable genotype JS 335.…”

Section: Plant Materialsmentioning

confidence: 99%

Genetics and mapping of seed coat impermeability in soybean using inter-specific populations

Chandra

Taak

Rathod

et al. 2020

Physiol Mol Biol Plants

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Seed coat impermeability (SCI) in soybean is associated with seed viability under storage and quality of processed products. Understanding genetics and identification of linked molecular markers would facilitate need-based utilization of seed coat impermeability. Two impermeable wild type (G. soja Sieb. and Zucc.) accessions viz. PI 424079 and PI 136620 were crossed with a permeable cultivated (G. max) variety JS335 to generate the mapping populations. Genetic analysis of the F 1:2 and F 2:3 seeds of the crosses indicated that SCI is controlled by a single gene/major QTL, and impermeability is dominant over permeability. Presence of seeds with intermediate permeability indicated role of some minor genes/QTLs. A set of 204 inter-specific recombinant inbred line (RILs) (F 7 ) was used to map SCI with 207 SSR markers. Phenotyping through rapid imbibition approach (seed imbibition for 6 h), seven QTLs were mapped on chromosomes (Chrs.) 2, 5, 12, 13 and 16 in the seeds stored for 1-3 years, while through slow imbibition method (seed imbibition for 7 days), five QTLs were mapped on Chrs. 2, 9, 10 and 20. Phenotypic variation explained (PVE) by the QTLs ranged from 5.96 to 39.67%. Two major and stable QTLs viz., qScI-h2-1 and qScI-h2-2 that mapped in tandem on Chr.2 jointly explained 43.09-62.92% of the variations in impermeability. Seven minor QTLs identified here were novel and two (qScI-h5, and qScI-h16) were consistent. It is the first report of mapping impermeability using two imbibition approaches together in 200 plus interspecific RILs in soybean. The study will pave the way for developing genotypes with restricted permeability, enhanced seed viability, and improved seeds quality.

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“…For one, the major and minor hilum axes lengths have been positively correlated with protein content and individual seed weight ( Barion et al, 2016 ). Plus, plant seeds with intact hilums exhibit relatively high seed vigor ( Kumar et al, 2019 ), whereas plants with injured hilums produce poor quality seeds that are susceptible to significant yield losses, possibly due to bacterial infections and reduced nutrient flows ( Hsieh et al, 2005 ). In addition, the seed hilum is also the channel for water uptake and efflux during germination ( Pietrzak et al, 2002 ; Zhang et al, 2004 ; Muramatsu et al, 2008 ; Jaganathan et al, 2019 ) and as the seed matures ( Hyde, 1954 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Common Genetic Basis Underlying Seed Hilum Size, Yield, and Quality Traits in Soybean

et al. 2021

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Developing high yielding cultivars with outstanding quality traits are perpetual objectives throughout crop breeding operations. Confoundingly, both of these breeding objectives typically involve working with complex quantitative traits that can be affected by genetic and environmental factors. Establishing correlations of these complex traits with more easily identifiable and highly heritable traits can simplify breeding processes. In this study, two parental soybean genotypes contrasting in seed hilum size, yield, and seed quality, as well as 175 F9 recombinant inbred lines (RILs) derived from these parents, were grown in 3 years. The h2b of four hilum size, two quality and two yield traits, ranged from 0.72 to 0.87. The four observed hilum size traits exhibited significant correlation (P < 0.05) with most of seed yield and quality traits, as indicated by correlation coefficients varying from -0.35 to 0.42, which suggests that hilum size could be considered as a proxy trait for soybean yield and quality. Interestingly, among 53 significant quantitative trait loci (QTLs) with logarithm of odds (LOD) values ranging from 2.51 to 6.69 and accounting for 6.40–16.10% of genetic variation, three loci encoding hilum size, qSH6.2, qSH8, and qSH10, colocated with QTLs for seed yield and quality traits, demonstrating that genes impacting seed hilum size colocalize in part with genes acting on soybean yield and quality. As a result of the breeding efforts and field observations described in this work, it is reasonable to conclude that optimizing hilum size through selection focused on a few QTLs may be useful for breeding new high yielding soybean varieties with favorable quality characteristics.

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Genetic studies on seed coat permeability and viability in RILs derived from an inter-specific cross of soybean [Glycine max (L.) Merrill]

Cited by 7 publications

References 12 publications

Seed longevity studies in wild type, cultivated and inter-specific recombination inbred lines (RILs) of soybean

Seed longevity studies in wild type, cultivated and inter-specific recombination inbred lines (RILs) of soybean

Genetics and mapping of seed coat impermeability in soybean using inter-specific populations

Characterization of the Common Genetic Basis Underlying Seed Hilum Size, Yield, and Quality Traits in Soybean

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