Genetic study of glutathione accumulation during cold hardening in wheat

Kocsy, Gábor; Szalai, Gabriella; Vágújfalvi, Attila; Stéhli, László; Orosz, György; Galiba, Gábor

doi:10.1007/pl00008137

Cited by 109 publications

(92 citation statements)

References 41 publications

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“…Strong evidence for a relationship between tissue glutathione and chilling tolerance in maize has come from several studies by Kocsy and co-workers. In particular, these authors have shown that chilling tolerance is accompanied by increased g-ECS activity and GSH accumulation (Kocsy et al, 1996(Kocsy et al, , 2000a(Kocsy et al, , 2000b(Kocsy et al, , 2001b. Hence, we cloned maize genes encoding g-ECS and GSH-S.…”

Section: Discussionmentioning

confidence: 99%

“…Leaf glutathione contents correlate with chilling resistance in maize (Kocsy et al, 1996(Kocsy et al, , 2000a(Kocsy et al, , 2000b(Kocsy et al, , 2001a(Kocsy et al, , 2001b. Furthermore, our previous data suggest that one factor in the sensitivity of maize to both shortterm and long-term chilling may be restrictions over glutathione reduction and cycling between cell types (Doulis et al, 1997;Pastori et al, 2000a).…”

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confidence: 85%

“…Avoidance of active oxygen species-induced cell death and senescence requires efficient antioxidant protection (Foyer and Noctor, 2000). Among the battery of antioxidants found in leaves, the tripeptide thiol glutathione (GSH; g-Glu-Cys-Gly) is strongly implicated in chilling tolerance, particularly in maize (Kocsy et al, 1996(Kocsy et al, , 2000a(Kocsy et al, , 2000b(Kocsy et al, , 2001a(Kocsy et al, , 2001b. Glutathione is the major thiol redox buffer in the soluble phase of most aerobic cells and in plants is involved in redox signaling, modulation of enzyme activity, control of root development, and in processes that modify and transport hormones and other endogenous compounds and xenobiotics, via formation of glutathione S-conjugates (May et al, 1998a;.…”

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Intercellular Distribution of Glutathione Synthesis in Maize Leaves and Its Response to Short-Term Chilling

et al. 2004

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To investigate the intercellular control of glutathione synthesis and its influence on leaf redox state in response to short-term chilling, genes encoding g-glutamylcysteine synthetase (g-ECS) and glutathione synthetase (GSH-S) were cloned from maize (Zea mays) and specific antibodies produced. These tools were used to provide the first information on the intercellular distribution of g-ECS and GSH-S transcript and protein in maize leaves, in both optimal conditions and chilling stress. A 2-d exposure to low growth temperatures (chill) had no effect on leaf phenotype, whereas return to optimal temperatures (recovery) caused extensive leaf bleaching. The chill did not affect total leaf GSH-S transcripts but strongly induced g-ECS mRNA, an effect reversed during recovery. The chilling-induced increase in g-ECS transcripts was not accompanied by enhanced total leaf g-ECS protein or extractable activity. In situ hybridization and immunolocalization of leaf sections showed that g-ECS and GSH-S transcripts and proteins were found in both the bundle sheath (BS) and the mesophyll cells under optimal conditions. Chilling increased g-ECS transcript and protein in the BS but not in the mesophyll cells. Increased BS g-ECS was correlated with a 2-fold increase in both leaf Cys and g-glutamylcysteine, but leaf total glutathione significantly increased only in the recovery period, when the reduced glutathione to glutathione disulfide ratio decreased 3-fold. Thus, while there was a specific increase in the potential contribution of the BS cells to glutathione synthesis during chilling, it did not result in enhanced leaf glutathione accumulation at low temperatures. Return to optimal temperatures allowed glutathione to increase, particularly glutathione disulfide, and this was associated with leaf chlorosis.

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confidence: 99%

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Intercellular Distribution of Glutathione Synthesis in Maize Leaves and Its Response to Short-Term Chilling

et al. 2004

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“…Sun et al [23] observed that no PC was detected in Sedum alfredii in a mine population; instead, Zn and Pb treatments resulted in an increase in GSH, indicating that GSH might be involved in Zn and Pb accumulation and tolerance. Abiotic stresses have been previously shown to enhance GSH accumulation in the leaves and roots of various species [24], [25]. Under Cu or Cd stress, the level of GSH in the nonaccumulator was always higher than that in the hyperaccumulator, which correlated with the changes in MDA content.…”

Section: Glutathione and Phytochelatinsmentioning

confidence: 93%

Cu and Cd induced Cytotoxicity Involving Lipid Peroxidation and Sulfhydryl Compounds in the Hyperaccumulator and Nonaccumulator Varieties of Commelina Communis

Hai-ou¹

2013

IJESD

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Abstract-The ability to accumulate Cu and Cd was investigated in Cu hyperaccumulator and nonaccumulator varieties of Commelina communis. Furthermore, the role of malondialdehyde (MDA), glutathione (GSH), and phytochelatin (PC) in the detoxification mechanisms used by the hyperaccumulator C. communis to cope with heavy metals were investigated. Results showed that Cu and Cd contents of both leaves and roots in the hyperaccumulator were higher than those in the nonaccumulator. However, the hyperaccumulator variety could have a more powerful ability to transport Cu and Cd from roots to shoots, thus decreasing the toxicity risk. Meanwhile, the MDA, GSH, and PC contents in the hyperaccumulator were significantly lower than those in the nonaccumulator under Cu and Cd stress, indicating that the former can utilize these sulfhydryl compounds to reduce toxicity caused by metal ions. Thus, the hyperaccumulator can tolerate heavy metal-induced toxicity better than the nonaccumulator.Index Terms-Heavy metals, malondialdehyde, glutathione, phytochelatin. I. INTRODUCTIONCu is an essential micronutrient for plants, a component of several electron transport enzymes, and is involved in catalyzing redox reactions in mitochondria and chloroplasts [1]. However, Cu also induces toxicity at tissue concentrations slightly above its optimal levels [2]. It can produce a mass of reactive oxygen species, damage cell membranes, and accumulate peroxide [3]. Glutathione (GSH) plays an important role in the heavy metal detoxification mechanism of plants [4]. GSH is a ubiquitous molecule with several roles in cell metabolism, including reactive oxygen species processing, redox state regulation, transport of amino acids, and sulfur storage [5], [6]. A function of GSH could be the chelation of toxic Cd (II) ions during their transport through the cytoplasm [7]. GSH is also used to synthesize phytochelatin (PC) [8]- [10]. Numerous physiological studies have indicated that the roles of PC include heavy metal detoxification [11] and maintenance of the homeostasis of intracellular levels of essential metal ions [12]. Malondialdehyde (MDA) is often used as an indicator of Manuscript received January 14, 2013; revised March 14, 2013 Commelina communis is commonly known in China as an annual multibranched herb with erect stems in its upper part and creeping stems in its lower part. Many studies have been performed to illustrate the hyperaccumulating mechanism of the hyperaccumulator variety of C. communis. Reports that describe the reaction of MDA, GSH, and PC to Cu, however, number much less. Moreover, scholars widely hypothesize that the hyperaccumulator can accumulate more than one kind of heavy metal. As such, two of varieties of C. communis were studied in our experiment as to whether or not they could also accumulate Cd in addition to Cu. The movement of MDA, GSH, and PC in the two varieties was further investigated under Cu-and Cd-induced stress. The study is an attempt to understand the mechanism by which the hyperaccumulator variety can tolera...

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“…Determination of thiols GR, protein and 35 S-radiolabelled compounds written in details by Kocsy et al (2000). The details of the western analysis of COR14b protein and the northern analysis of cor14b mRNA are described by Crosatti et al (1999).…”

Section: Biochemical and Molecular Analysismentioning

confidence: 99%

Mapping of Genes Involved in Glutathione, Carbohydrate and COR14b Cold Induced Protein Accumulation during Cold Hardening in Wheat

Galiba¹,

Kerepesi²,

Vágújfalvi³

et al. 2001

Wheat in a Global Environment

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Using some of the chromosome substitution lines developed from the crosses of the donor Cheyenne to Chinese Spring we showed that the accumulation of water soluble carbohydrates during different stages of hardening was time dependent. Moreover there was a significant correlation between the rate of carbohydrate accumulation and the frost tolerance. The expression and regulation of a wheat gene homologous to the barley cold regulated cor14b gene was compared in frost sensitive and frost tolerant wheat genotypes at different temperatures. Studies made with chromosome substitution lines showed that the threshold induction temperature polymorphism of the cor14b wheat homologous gene was controlled by loci located on chromosome 5A of wheat, while cor14b gene was mapped, in Triticum monococcum, onto the long arm of chromosome 2A m . Our study on the effect of cold hardening on glutathione (GSH) metabolism showed that chromosome 5A of wheat has an influence on the GSH accumulation and on the ratio of reduced and oxidised glutathione as part of a complex regulatory function during cold hardening. In addition, the level of increase in GSH content during hardening may indicate the degree of the frost tolerance of wheat.

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Genetic study of glutathione accumulation during cold hardening in wheat

Cited by 109 publications

References 41 publications

Intercellular Distribution of Glutathione Synthesis in Maize Leaves and Its Response to Short-Term Chilling

Intercellular Distribution of Glutathione Synthesis in Maize Leaves and Its Response to Short-Term Chilling

Cu and Cd induced Cytotoxicity Involving Lipid Peroxidation and Sulfhydryl Compounds in the Hyperaccumulator and Nonaccumulator Varieties of Commelina Communis

Mapping of Genes Involved in Glutathione, Carbohydrate and COR14b Cold Induced Protein Accumulation during Cold Hardening in Wheat

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