Genetic Transformation and Transgenics of Jatropha curcas, a Biofuel Plant

Tolerance to salinity in Jatropha curcas are genotype-dependent

“…The complete recovery of gas exchange in JCAL171, CNPAE114, and CNPAE112 genotypes confirm our hypothesis that J. curcas is strongly tolerant to salinity, considered by some authors (Díaz-López et al 2012a;Fu et al 2019) like a halophyte plant. The accumulation of Na + in the leaves in the genotypes on maximum stress revealed to be an important indicator of behavior from different genotypes compared to saline stress.…”

“…The high concentrations of ions Clon leaves may cause degradation of chlorophyll, causing damage on the photosystems (Asada 1999;Pompelli et al 2010c;Tavakkoli et al 2011), favoring the fall of photosynthesis rate and damage to the photosynthetic apparatus of some genotypes studied. Díaz-López et al (2012a) suggests that J. curcas seedlings exhibit a moderate tolerance to salinity, as the plants were able to tolerate up to 4 dS m -1 , equivalent to 30 mM NaCl, while Fu et al (2019) described that a transgenic J. curcas expressing sbNHX1 gene, which encodes an active vacuolar Na + /H + antiporter could enhance tolerance up to 200 mM NaCl. Our study contradicts these studies since in this study, we tested 25x greater than salt concentration tested by Díaz-López et al (2012a) or 4 times greater than salt concentration described by Fu et al (2019).…”

“…Díaz-López et al (2012a) suggests that J. curcas seedlings exhibit a moderate tolerance to salinity, as the plants were able to tolerate up to 4 dS m -1 , equivalent to 30 mM NaCl, while Fu et al (2019) described that a transgenic J. curcas expressing sbNHX1 gene, which encodes an active vacuolar Na + /H + antiporter could enhance tolerance up to 200 mM NaCl. Our study contradicts these studies since in this study, we tested 25x greater than salt concentration tested by Díaz-López et al (2012a) or 4 times greater than salt concentration described by Fu et al (2019).…”

Modulation of photosynthesis under salinity and the role of mineral nutrients in Jatropha curcas L.

“…Díaz‐López et al (2012) suggest that J . curcas seedlings exhibit a moderate tolerance to salinity, as the plants were able to tolerate up to 4 dS m −1 , equivalent to 30‐mM NaCl, while Fu et al (2019) described that a transgenic J . curcas expressing sbNHX1 gene, which encodes an active vacuolar Na + /H + antiporter, could enhance tolerance up to 200‐mM NaCl.…”

“…, favouring the fall of photosynthesis rate and damage to the photosynthetic apparatus of some genotypes studied Díaz-López et al (2012). suggest that J. curcas seedlings exhibit a moderate tolerance to salinity, as the plants were able to tolerate up to 4 dS m −1 , equivalent to 30-mM NaCl, whileFu et al (2019) described that a transgenic J. curcas expressing sbNHX1 gene, which encodes an active vacuolar Na + /H + antiporter, could enhance tolerance up to 200-mM NaCl. More recently, our research group(Corte-Real et al, 2019, 2020Lozano-Isla et al, 2018;Silva-Santos et al, 2019) described that J. curcas can grow and sometimes produce fruits, even under 750-mM NaCl in irrigation water.Here, we also show that several mineral elements can favour or decrease A N , in addition to Na + and Cl − ions.…”