Genetic Transformation in Ipomoea batatas (L.) Lam (Sweet Potato)

Lowe, J.; Hamilton, W. D.; Newell, C. A.

doi:10.1007/978-3-662-09366-5_21

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…Sweet potato transformation has been attempted by electroporation Mitchell et al 1998;Lawton et al 2000;Okada et al 2001), particle bombardment (Prakash and Varadarajan 1992), and Agrobacterium rhizogenes (Otani et al 1993). These methods, however, were found to be ineffective because of the frequent appearance of morphological abnormalities, transformation without regeneration, low reproducibility or efficiency, and genotype dependency (Lowe et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Transgenic sweet potato expressing mammalian cytochrome P450

Anwar

Watanabe

2010

Plant Cell Tiss Organ Cult

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Sweet potato [Ipomoea batatas (L.) Lam] is considered to be recalcitrant to transformation and regeneration because of its genotype-dependent in vitro responses. The lack of a genotype-independent transformation and regeneration system limits biotechnological applications in this plant species. To establish a transformation system for a diverse group of sweet potato genotypes, we examined sweet potato regeneration after transformation in five cultivars. An Agrobacterium tumefaciens transformation system was used for the introduction of mammalian cytochrome P450 genes, which are capable of conferring herbicide tolerance. Among the different factors studied, including explant type, plasmid vectors, and auxin type in the initiation media, auxin type had the greatest effect on the regeneration response. Of the auxins tested, only 4-fluorophenoxyacetic acid (4FA) induced regeneration from all cultivars. In terms of the quality of calli, 4FA promoted the induction of type I calli, which were capable of somatic embryo formation, whereas type II calli fail to produce somatic embryos. The frequency of somatic embryo formation was also affected by the composition of the embryo-induction media. Transgenic plants were regenerated from all cultivars. The stable integration and expression of transgenes was confirmed by several approaches. This Agrobacterium-mediated transformation system should be applicable to a wide range of sweet potato cultivars.

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Section: Introductionmentioning

confidence: 99%

Transgenic sweet potato expressing mammalian cytochrome P450

Anwar

Watanabe

2010

Plant Cell Tiss Organ Cult

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“…A. tumefaciens mediated transformation in sweet potato was well established by several workers (Al-Juboory and Skirvin 1991, Carelli et al 1991, Prakash and Varadarajan 1991, Lowe et al 1994, Otani et al 2001. In general, these procedures have been mostly genotype-dependent with lower transformation efficiency (Otani et al 2003, Song et al 2004, Shimada et al 2006, Luo et al 2006, and often difficult to reproduce (Lowe et al 1994). Agrobacterium-mediated transformation in sweet potato has also been applied for regeneration via somatic embryogenesis using somatic embryos or organs as explants by several workers (Pido et al 1995, Newell et al 1995, Gama et al 1996, Otani et al 1998, Luo et al 2006).…”

Section: Resultsmentioning

confidence: 99%

“…However, morphological abnormalities shown by regenerated transgenic plants were a big question mark for the method. A. tumefaciens mediated transformation in sweet potato was well established by several workers (Al-Juboory and Skirvin 1991, Carelli et al 1991, Prakash and Varadarajan 1991, Lowe et al 1994, Otani et al 2001. In general, these procedures have been mostly genotype-dependent with lower transformation efficiency (Otani et al 2003, Song et al 2004, Shimada et al 2006, Luo et al 2006, and often difficult to reproduce (Lowe et al 1994).…”

Section: Resultsmentioning

confidence: 99%

Genotype Independent Regeneration and Agrobacterium?mediated Genetic Transformation of Sweet Potato (Ipomoea batatas L.)

Shekhar

Agrawal

Buragohain

et al. 2013

Plant Tissue Cult. & Biotech.

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Development of an efficient genotype independent regeneration and genetic transformation system in sweet potato continues to be of great interest. Agrobacterium-mediated genetic transformation protocol was established in two different cultivars of sweet potato using Agrobacterium strain EHA105 harbouring binary plasmid pBI121 containing GUS and nptII genes. The internodal stem segments from 30-day-old micropropogated plants were used as explant with different combinations of media and hormones. MS and LS media with various concentrations of growth regulators proved to be non-responsive and the infecundity was severe with the addition of cytokinins. Nonetheless, MS with 2,4-D and TDZ gave a good percentage of callusing but with low differentiation. In different concentrations of NAA, significant amount of callusing was observed but percentage of rooting remained low in both the genotypes. Gamborg's B5 supplemented with NAA proved to be the most suitable media and hormone combination, which yielded shoot formation after 8 -10 weeks with a regeneration efficiency of 40 -70%. Stable integration of transgene was confirmed by PCR analysis. Furthermore, qRT-PCR analysis was performed to assess the transcript accumulation in addition to the GUS enzymatic assay in the transgenic lines.

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