Genetic Transformation in Prunus persica (Peach) and Prunus domestica (Plum)

Scorza, R.; Hammerschlag, F. A.; Zimmerman, Thomas W.; Cordts, John

doi:10.1007/978-3-642-57840-3_23

Cited by 14 publications

(13 citation statements)

References 27 publications

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“…Meristem transformation may eliminate the need for regeneration in production of transgenic plants, allowing genetic manipulation of established cultivars. However, high explant mortality and difficulties controlling Agrobacterium growth have limited the development of this methodology (Scorza et al, 1995a). Recently, a reliable procedure for transformation of different grape cultivars has been developed (Mezzetti et al, 2002).…”

Section: Shortcomings In the Transformation Of Fruit Treesmentioning

confidence: 99%

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Transformation of fruit trees. Useful breeding tool or continued future prospect?

2005

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Regeneration and transformation systems using mature plant material of woody fruit species have to be achieved as a necessary requirement for the introduction of useful genes into specific cultivars and the rapid evaluation of resulting horticultural traits. Although the commercial production of transgenic annual crops is a reality, commercial genetically-engineered fruit trees are still far from common. In most woody fruit species, transformation and regeneration of commercial cultivars are not routine, generally being limited to a few genotypes or to seedlings. The future of genetic transformation as a tool for the breeding of fruit trees requires the development of genotype-independent procedures, based on the transformation of meristematic cells with high regeneration potential and/or the use of regeneration-promoting genes. The public concern with the introduction of antibiotic resistance into food and the restrictions due to new European laws that do not allow deliberate release of plants transformed with antibiotic-resistance genes highlight the development of methods that avoid the use of antibiotic-dependent selection or allow elimination of marker genesfrom the transformed plant as a research priority in coming years.

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Section: Shortcomings In the Transformation Of Fruit Treesmentioning

confidence: 99%

“…In all cases of integration of ''genes of interest'', hypocotyls have been used for transformation (Laimer da Caˆmara Machado et al, 1992;Scorza et al, 1995a), with the exception of the transformation of commercial cherry rootstocks with the T-DNA from A. rhizogenes (Gutie´rrez-Pesce et al, 1998).…”

Section: Shortcomings In the Transformation Of Fruit Treesmentioning

confidence: 99%

Transformation of fruit trees. Useful breeding tool or continued future prospect?

2005

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“…Since our initial report of hypocotyl transformation of plum (Prunus domestica L.) (Mante et al 1991), we have successfully used this technology to transfer various transgenes, including the NPTII and GUS marker genes, and viral CP genes into this species (Scorza et al 1994(Scorza et al , 1995a(Scorza et al , 1995b. This method utilizes MS medium (Murashige and Skoog 1962) supplemented with thidiazuron (TDZ) and indole-3-butyric acid (IBA) in ratios ranging from 2:1 to 5:1.…”

Section: Introductionmentioning

confidence: 98%

Early antibiotic selection and efficient rooting and acclimatization improve the production of transgenic plum plants ( Prunus domestica L.)

2003

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We describe here an improved system for routinely developing transgenic plum plants (Prunus domestica L.) through the use of Agrobacterium tumefaciens. The production of non-transformed "escapes" has been virtually eliminated, and rates of plant establishment in the greenhouse have been dramatically improved. The system is based on the regeneration of shoots from hypocotyls extracted from mature seed. The shoot regeneration medium is Murashige and Skoog (MS) salts and vitamins supplemented with 7.5 microM thidiazuron and 0.25 microM indole-butyric acid. Transferring the explants after co-cultivation to shoot regeneration medium containing 80 mg l(-1) of kanamycin and 300 mg l(-1) of Timentin reduced the total number of regenerated shoots without affecting the transformation rate. Transformation rates using the described system averaged 1.2% of the hypocotyl slices producing transgenic plants, with a range of 0-4.2%. The transgenic shoots rooted at a rate of 90% on half-strength MS salts and vitamins supplemented with 5 microM alpha-naphthaleneacetic acid and 0.01 microM kinetin. Plantlets were transferred to a greenhouse directly from culture tubes with a 90% average survival.

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“…Cut the hypocotyl into three slices as shown in Figure 1C. Epicotyls are used for propagation via axillary bud development and hypocotyls are used for propagation via adventitious shoot induction (Scorza et al,1995;Tian et al, 2004;2005;Sibbald et al, 2006).…”

Section: Explant Dissectionmentioning

confidence: 99%

“…The genetic composition of these two species is also different. P. domestica is a hexaploid plant; however, P. salicina (and many other plum species), has a diploid genetic background (Scorza et al, 1995;Okie & Ramming, 1999).…”

Section: Introductionmentioning

confidence: 98%