Genetic transformation of Cavendish banana ( Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment

Becker, Douglas K.; Dugdale, Benjamin; Smith, Malcolm W.; Harding, Robert M.; Dale, James L.

doi:10.1007/s002990050004

Cited by 132 publications

(57 citation statements)

References 14 publications

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“…Other cultivars from laboratories include the East African Highland banana, Gonja (plantain), and Rasthali. Additionally, efficient transformation protocols are available for bananas, for both Agrobacterium-mediated transformation and microprojectile bombardment [49][50][51]. There are a number of laboratories around the world that have established banana transformation pipelines, including ours at Queensland University of Technology (https://www.qut.edu.au/), the IITA in Kenya (http://newint.iita.org/), NARO in Uganda (http://www.naro.go.ug/), the Bhabha Atomic Research Centre in India (http://www.barc.gov.in/), and the Catholic University of Leuven in Belgium (https://www.kuleuven.be/english).…”

Section: Conventional Breeding or Genetic Modificationmentioning

confidence: 99%

Modifying Bananas: From Transgenics to Organics?

et al. 2017

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Bananas are one of the top ten world food crops. Unlike most other major food crops, bananas are difficult to genetically improve. The challenge is that nearly all banana cultivars and landraces are triploids, with high levels of male and female infertility. There are a number of international conventional breeding programs and many of these are developing new cultivars. However, it is virtually impossible to backcross bananas, thus excluding the possibility of introgressing new traits into a current cultivar. The alternative strategy is to "modify" the cultivar itself. We have been developing the capacity to modify Cavendish bananas and other cultivars for both disease resistance and enhanced fruit quality. Initially, we were using transgenes; genes that were derived from species outside of the Musa or banana genus. However, we have recently incorporated two banana genes (cisgenes) into Cavendish; one to enhance the level of pro-vitamin A and the other to increase the resistance to Panama disease. Modified Cavendish with these cisgenes have been employed in a field trial. Almost certainly, the next advance will be to edit the Cavendish genome, to generate the desired traits. As these banana cultivars are essentially sterile, transgene flow and the outcrossing of modified genes into wild Musa species. are highly unlikely and virtually impossible in other triploid cultivars. Therefore, genetic changes in bananas may be compatible with organic farming.

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Section: Conventional Breeding or Genetic Modificationmentioning

confidence: 99%

Modifying Bananas: From Transgenics to Organics?

et al. 2017

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“…After bombardment, 62% of apical meristems were survived on the selective medium and 80% of explants produced shoots in the following first subculture and all shoots were rooted (Ismail et al, 2011). In addition to those disease-based studies, others were also carried out in order to develop efficient transformation protocols via biolistic transformation (Sagi et al, 1995;Becker et al, 2000). Among them, Sagi and co-workers (1995) reported the transformation of embryogenic cell suspensions of cooking banana 'Bluggoe' (ABB genome) and plantain 'Three Hand Planty' (AAB genome) via particle bombardment.…”

Section: Direct Gene Transfermentioning

confidence: 99%

“…Among them, PDS-1000/He, Biolistic® particle delivery system (BIO-RAD), which is a modified version of Sanford's system, is the most used system for biolistic transformation due to its efficient and relatively simple application and acquisition of reproducible results between laboratories (Taylor & Fauquet, 2002). Particle inflow gun can be an alternative to other biolistic systems due to its very low cost and it was used successfully in banana transformation (Becker et al, 2000). Electrical discharge particle acceleration, ACCELL™ technology uses high voltage electrical discharge into a droplet water to generate shock waves and project microprojectiles to different cell layers of target tissues (McCabe & Christou, 1993).…”

Section: Direct Gene Transfermentioning

confidence: 99%

“…Then, Cavendish banana cv. Grand Nain embryogenic suspension cells were co-bombarded with the plasmid containing nptII selectable marker gene under the control of BBTV promoter or the cauliflower mozaic virus (CaMV) 35S promoter, the -glucuronidase (gusA) reporter gene and BBTV genes under the control of the maize polyubiquitin promoter by using particle inflow gun and stably integration was obtained in all of the tested transformed plants (Becker et al, 2000). Very recently, microprojectile bombardment was also applied to induce tolerance to Sigatoka leaf spot caused by Mycosphaerella fijiensis in banana by transferring endochitinase gene of ThEn-42 from Trichoderma harzianum together with the grape stilbene synthase gene (StSy) under the control of 35 S promoter and the inducible PR-10 promoter, respectively (Vishenevetsky et al, 2011).…”

Section: Direct Gene Transfermentioning

confidence: 99%

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Recent Advances in Fruit Species Transformation

Akdemir¹,

Gago²,

Gallego³

et al. 2012

Transgenic Plants - Advances and Limitations

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“…'Grande Naine' has been stably transformed and regenerated via microprojectile bombardment of embryogenic suspension cells derived from immature male flowers (Becker et al, 2000). Stable transformation and regeneration have been achieved in the cultivars 'Williams' (AAA), 'Three Hand Planty' and 'Maçã' (AAB), and 'Bluggoe' (ABB) (Remy et al, 1998a(Remy et al, , 1998bSwennen et al, 1998 Swennen et al, , 2003 Sági et al, 2000; Matsumoto et al, 2002a), and in the tetraploid cultivar FHIA-21 (AAAB) (Daniels et al, 2002).…”

Section: Genetic Engineeringmentioning

confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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io s a fe t y nv ir on me nt ag ri cu lt ur e bi os af et y g r ic u lt u r e e n vi r o n m e n t gr ic ul tu re bi os af et y en vi ro nm en t n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en v io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et...

show abstract

Genetic transformation of Cavendish banana ( Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment

Cited by 132 publications

References 14 publications

Modifying Bananas: From Transgenics to Organics?

Modifying Bananas: From Transgenics to Organics?

Recent Advances in Fruit Species Transformation

Safety Assessment of Transgenic Organisms, Volume 4

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