Genetic transformation of commercial cultivars of oat ( Avena sativa L.) and barley ( Hordeum vulgare L.) using in vitro shoot meristematic cultures derived from germinated seedlings

Zhang, S.; Cho, M.-J.; Koprek, Thomas; Yun, Ruixin; Bregitzer, Phil; Lemaux, Peggy G.

doi:10.1007/s002990050691

Cited by 103 publications

(79 citation statements)

References 28 publications

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“…In this case, the relationship between the hemi-and homozygous transgene expression level, already documented for rice (James et al 2002) could explain the distorted segregation. The irregular pattern of transgene expression and nonMendelian inheritance of the trait in oat transformed by the biolistic method was also reported for transgenes driven by the Adh1, 35S ) and Act1 (Zhang et al 1999) promoters. However, in these reports, some of the instabilities were caused by the method itself.…”

Section: Transgene Expressionmentioning

confidence: 60%

“…Oat (Avena sativa L.) is another allohexaploid species thus far transformed only via the biolistic method. Its genetic transformation was accomplished using embryogenic calli or cell suspensions derived from immature embryos (Kuai et al 2001;Perret et al 2003;Somers et al 1992;Torbert et al 1995Torbert et al , 1998a, mature embryos (Kaeppler et al 2000;Torbert et al 1998b), leaf base segments (Gless et al 1998b), cultures from seeds , and shoot apical meristems (Cho et al 2003;Maqbool et al 2002;Zhang et al 1999). Obtaining transgenic plants from leaf base segments has thus far only been possible for oat; it is not yet applicable to other allohexaploid cereals (Sticklen and Oraby 2005).…”

Section: Introductionmentioning

confidence: 99%

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Agrobacterium-mediated transformation of oat (Avena sativa L.) cultivars via immature embryo and leaf explants

et al. 2008

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This paper reports on the successful Agrobacterium-mediated transformation of oat, and on some factors influencing this process. In the first step of the experiments, three cultivars, two types of explant, and three combinations of strain/vectors, which were successfully used for transformation of other cereals were tested. Transgenic plants were obtained from the immature embryos of cvs. Bajka, Slawko and Akt and from leaf base explants of cv. Bajka after transformation with A. thumefaciens strain LBA4404(pTOK233). The highest transformation rate (12.3%) was obtained for immature embryos of cv. Bajka. About 79% of the selected plants proved to be transgenic; however, only 14.3% of the T 0 plants and 27.5% of the T 1 showed GUS expression. Cell competence of both types of explant differed in terms of their transformation ability and transgene expression. The next step of the study was to test the suitability for oat transformation of the pGreen binary vector combined with different selection cassettes: nptII or bar under the nos or 35S promoter. Transgenic plants were selected in combinations transformed with nos::nptII, 35S::nptII and nos::bar. The highest transformation efficiency (5.3%) was obtained for cv. Akt transformed with nos::nptII. A detailed analysis of the T 0 plants selected from a given callus line and their progeny revealed that they were the mixture of transgenic, chimeric-transgenic and non-transgenic individuals. Southern blot analysis of T 0 and T 1 showed simple integration pattern with the low copy number of the introduced transgenes.

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Section: Transgene Expressionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of oat (Avena sativa L.) cultivars via immature embryo and leaf explants

et al. 2008

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“…Transformation with alternative target tissues, such as shoot meristematic cultures (Zhang et al, 1999), androgenetic pollen cultures (Jähne et al, 1994;Yao et al, 1997;Kumlehn et al, 2006), in vitro cultured ovules (Holme et al, 2008), and mature embryos (Sharma et al, 2005), have also been attempted. Shoot meristematic cultures from germinating seedlings of barley cv.…”

Section: Cereal Crop Transformation 501mentioning

confidence: 99%

“…Shoot meristematic cultures from germinating seedlings of barley cv. Harrington have been used for the biolistic delivery of the bar/nptII and gus genes (Zhang et al, 1999). However, the TF was very low because only one fertile transgenic barley plant with stable expression was obtained from all six bombardments from a total of 240 explants (Zhang et al, 1999).…”

Section: Cereal Crop Transformation 501mentioning

confidence: 99%

“…Harrington have been used for the biolistic delivery of the bar/nptII and gus genes (Zhang et al, 1999). However, the TF was very low because only one fertile transgenic barley plant with stable expression was obtained from all six bombardments from a total of 240 explants (Zhang et al, 1999). Mature embryos also proved to be good targets in both callus induction and regeneration among seven barley cultivars tested (Sharma et al, 2005), hinting at the potential of mature embryos for transformation, but this explant was almost never mentioned in the literature.…”

Section: Cereal Crop Transformation 501mentioning

confidence: 99%

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Genetic transformation of major cereal crops

Ji¹,

Xu²,

Wang³

2013

Int. J. Dev. Biol.

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Of the more than 50,000 edible plant species in the world, at least 10,000 species are cereal grains. Three major cereal crops, rice (Oryza sativa), maize (Zea mays), and wheat (Triticum sp.), provide two-thirds of the world's food energy intake. Although crop yields have improved tremendously thanks to technological advances in the past 50 years, population increases and climate changes continue to threaten the sustainability of current crop productions. Whereas conventional and marker-assisted breeding programs continue to play a major role in crop improvement, genetic engineering has drawn an intense worldwide interest from the scientific community. In the past decade, genetic transformation technologies have revolutionized agricultural practices and millions of hectares of biotech crops have been cultured. Because of its unique ability to insert well-characterized gene sequences into the plant genome, genetic engineering can also provide effective tools to address fundamental biological questions. This technology is expected to continue to be an indispensable approach for both basic and applied research. Here, we overview briefly the development of the genetic transformation in the top seven cereals, namely maize, rice, wheat, barley (Hordeum vulgare), sorghum (Sorghum sp.), oat (Avena sativa), and millets. The advantages and disadvantages of the two major transformation methods, Agrobacterium tumefaciens-mediated and biolistic methods, are also discussed.

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Barley Biotechnology: Breeding and Transgenics

Newman

2008

Barley for Food and Health

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Genetic transformation of commercial cultivars of oat ( Avena sativa L.) and barley ( Hordeum vulgare L.) using in vitro shoot meristematic cultures derived from germinated seedlings

Cited by 103 publications

References 28 publications

Agrobacterium-mediated transformation of oat (Avena sativa L.) cultivars via immature embryo and leaf explants

Agrobacterium-mediated transformation of oat (Avena sativa L.) cultivars via immature embryo and leaf explants

Genetic transformation of major cereal crops

Barley Biotechnology: Breeding and Transgenics

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