Genetic transformation of green-colored cotton

Zhu, Sheng-Wei; Gao, Peng; Sun, Jun; Wang, Haihua; Luo, X. M.; Jiao, Ming-Yu; Wang, Zhiyong; Xia, Gui-Xian

doi:10.1079/ivp2006777

Cited by 26 publications

(7 citation statements)

References 23 publications

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“…28.23% efficiency of transformation was achieved [120]. An efficient method with improved frequency of somatic embryogenesis and concomitant growth of somatic embryos was developed.…”

Section: Transformation and Regeneration Of Cotyledonary Tissues Of Cmentioning

confidence: 99%

The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

Aslam¹,

Khan²,

Ahmed³

et al. 2020

AJMB

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Cotton is considered as a major cash crop of the world. It earns huge foreign exchange by its valuable products; fiber, lint, cotton seed oil, hull and a lot more. Being an important fiber crop, it earns huge foreign exchange by contributing to textile and seed oil industry. This review summarizes cotton biology, its diversity and domestication, genome assembly, constraints in its production and methods to improve cotton plant to fulfill the need of textile and oil industry. But cotton is facing enormous biotic and abiotic stresses with insect pests being most prominent. Massive destruction caused by insects needs to be controlled for maintaining fruitful cotton crop production. Conventional breeding approaches are limited to improving single trait and integrate stable genes within plant genome in approximately 7-8 years. Improved biotechnological procedures have paved new pathways to target genes specifically and improve cotton germplasm in lesser time than conventional breeding.

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“…28.23% efficiency of transformation was achieved [120]. An efficient method with improved frequency of somatic embryogenesis and concomitant growth of somatic embryos was developed.…”

Section: Transformation and Regeneration Of Cotyledonary Tissues Of Cmentioning

confidence: 99%

The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

Aslam¹,

Khan²,

Ahmed³

et al. 2020

AJMB

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“…Colored cotton (Gossypium hirsutum L.) has received increasing attention in recent years because it reduces the cost for dying, creates less environmental pollution, and is beneficial to human health (Hua et al, 2007;Yuan et al, 2012;Zhu et al, 2006). However, poor quality has limited the further development of colored cotton (Yuan et al, 2012;Zhang et al, 2010).…”

Section: Introduction `mentioning

confidence: 99%

Sucrose Metabolism for Cellulose Biosynthesis in Colored Cotton Fibers

Zhang¹

2017

PAKJAS

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Assays were conducted to examine the contents of cellulose and callose, and the activities of enzymes related to sucrose metabolism at various stages of fiber development in colored cottons and white cotton. The results showed that the cellulose accumulation could be described with Richard curve equations, and the cellulose biosynthesis was reflected by the eigenvalues, especially the maximum biosynthesis rate (Vmax) and the time taken for cellulose rapid biosynthesis (T). The period from 20 days post anthesis (DPA) to 40 DPA is the key stage for cellulose accumulation in colored cotton. During this period reduced activity acid invertase (AI) and β-1,3-glucanase, and decreased accumulation and transformation of callose in colored cotton might limit carbon supply and cellulose accumulation by affecting Vmax and T, thus inhibiting sucrose metabolism during cellulose biosynthesis, then results in poor fiber quality.

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“…The two most common fiber colors in colored cotton cultivars ( Gossypium hirsutum ) are “brown and green.” They are eco-friendly, economical, and valuable for human health (Zhu et al, 2006 ) Four key genes of the flavonoid biosynthetic pathway ( GhC4H, GhCHS, GhF3 ′ H , and GhF3 ′ 5 ′ H, GhDFR ) have been reported in Gossypium hirsutum .…”

Section: Introductionmentioning

confidence: 99%

In silico study for diversing the molecular pathway of pigment formation: an alternative to manual coloring in cotton fibers

et al. 2015

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Diversity of colors in flowers and fruits is largely due to anthocyanin pigments. The flavonoid/anthocyanin pathway has been most extensively studied. Dihydroflavonol 4-reductase (DFR) is a vital enzyme of the flavonoid pathway which displays major impact on the formation of anthocyanins, flavan 3-ols and flavonols. The substrate specificity of the DFR was found to play a crucial role in determination of type of anthocyanidins. Altering the flavonoid/anthocyanin pathway through genetic engineering to develop color of our own choice is an exciting subject of future research. In the present study, comparison among four DFR genes (Gossypium hirsutum, Iris × hollandica, Ang. DFRI and DFRII), sequence alignment for homology as well as protein modeling and docking is demonstrated. Estimation of catalytic sites, prediction of substrate preference and protein docking were the key features of this article. For specific substrate uptake, a proline rich region and positions 12 plus 26 along with other positions emphasizing the 26-amino acid residue region (132–157) was tested. Results showed that proline rich region position 12, 26, and 132–157 plays an important role in selective attachment of DFRs with respective substrates. Further, “Expasy ProtParam tool” results showed that Iris × hollandica DFR amino acids (Asn 9: Asp 23) are favorable for reducing DHQ and DHM thus accumulating delphinidin, while Gossypium hirsutum DFR has (Asn 13: Asp 21) hypothesized to consume DHK. Protein docking data showed that amino acid residues in above mentioned positions were just involved in attachment of DFR with substrate and had no role in specific substrate uptake. Advanced bioinformatics analysis has revealed that all above mentioned positions have role in substrate attachment. For substrate specificity, other residues region is involved. It will help in color manipulations in different plant species.

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Genetic transformation of green-colored cotton

Cited by 26 publications

References 23 publications

The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

Sucrose Metabolism for Cellulose Biosynthesis in Colored Cotton Fibers

In silico study for diversing the molecular pathway of pigment formation: an alternative to manual coloring in cotton fibers

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