Genetic transformation of Rangpur lime (Citrus limonia osbeck) with thebO (bacterio-opsin) genen and its initial evaluation forPhytophthora nicotianae resistance

The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.

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“…Bras. Frutic., Jaboticabal, 2019, v. 41, n. 2: (e-116) efficiency was lower than 2.5% (GUTIÉRREZ et al, 1997;AZEVEDO et al, 2006).…”

Section: Genetic Transformationmentioning

confidence: 92%

In vitro organogenesis and genetic transformation of mandarin cultivars

et al. 2019

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“…The bO gene is derived from Halobacteria halobium and can spontaneously activate programmed cell death and enhance broad-spectrum disease resistance accompanied by pathogenesis-related (PR) protein accumulation. In two of the transgenic lines, higher levels of tolerance to this pathogen with significantly smaller lesions were observed; however, these lines also exhibited HR-like lesions in the absence of pathogen (Azevedo et al, 2006). It remains to be seen if this strategy will work under field conditions given the fact that the transgenic plants develop spontaneous lesions.…”

Section: Pathogen-derived Genesmentioning

confidence: 99%

“…In particular, Phytophthora spp can cause root rot and gummosis in mature trees and damping-off in seedlings. For the control of Phytophthora nicotianae Azevedo et al (2006) used a bacterio-opsin (bO) gene to transform 'Rangpur' lime. The bO gene is derived from Halobacteria halobium and can spontaneously activate programmed cell death and enhance broad-spectrum disease resistance accompanied by pathogenesis-related (PR) protein accumulation.…”

Section: Pathogen-derived Genesmentioning

confidence: 99%

Citrus Transformation: Challenges and Prospects

Febres¹,

Fisher²,

Khalaf³

et al. 2011

Genetic Transformation

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“…Some observations made by Dr. Sylvio Moreira and Dr. Victória Rossetti suggested that orange plants that had been previously grafted on 'Rangpur' lime (Citrus limonia L.) rootstocks had escaped that disease, which was later confirmed, as evidenced by the 'Rangpur' lime resistance to CTV. Since then, 'Rangpur' lime has been a widely used citrus rootstock species for orange production in Brazil (Azevedo et al, 2006). But by the beginning of the year 2000, another threat, called citrus sudden death, was discovered to affect plants grafted on the 'Rangpur' lime rootstock.…”

Section: Root System Water Absorption/use Strategies In Citrus and Cmentioning

confidence: 99%