Genetic transformation of the apple rootstock M26 with the RolB gene and its influence on rooting

Welander, Margareta; Pawlicki, Nathalie; Holefors, Anna; Wilson, Frank J.

doi:10.1016/s0176-1617(98)80164-8

Cited by 60 publications

(36 citation statements)

References 34 publications

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“…Previous findings have showed that the addition of IBA at optimum levels enhanced the growth of hairy root culture of Echinacea but had no effect on the production of secondary metabolites (Choffe et al 2000;Koroch et al 2002;Staniszewska et al 2003;Washida et al 2004). The TL-DNA rolB gene does not increase auxin levels (Nilsson et al 1993) but increases the sensitivity of plant cells to the growth regulator (Cardarelli et al 1987;Shen et al 1988;Spanó et al 1988;Maurel et al 1991;Welander et al 1998), probably by enhanced binding of the auxin to plant cell membranes (Filippini et al 1994), consequently increasing the rooting efficiency. The changes in sensitivity and content of endogenous growth regulators in plant cells undoubtedly affected the response effect of plant cells to exogenous growth regulators (He-Ping et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Upon transfer to the mentioned medium, shoot regeneration from callus of transformed roots were obtained in these cultures after 40-50 days but they could not develop to mature shoots. It seems that the concentration of cefotaxime is not toxic to the explants, there might be an interaction between the explant and the bacteria which negatively affects shoot regeneration (Maurel et al 1991;Welander et al 1998). Thus, plants can be regenerated from hairy root cultures either spontaneously (directly from roots) or by transferring roots to hormone-containing medium.…”

Section: Discussionmentioning

confidence: 99%

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The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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We have developed an efficient transformation system for Tribulus terrestris L., an important medicinal plant, using Agrobacterium rhizogenes strains AR15834 and GMI9534 to generate hairy roots. Hairy roots were formed directly from the cut edges of leaf explants 10-14 days after inoculation with the Agrobacterium with highest frequency transformation being 49 %, which was achieved using Agrobacterium rhizogenes AR15834 on hormone-free MS medium after 28 days inoculation. PCR analysis showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. Isolated transgenic hairy roots grew rapidly on MS medium supplemented with indole-3-butyric acid. They showed characteristics of transformed roots such as fast growth and high lateral branching in comparison with untransformed roots. Isolated control and transgenic hairy roots grown in liquid medium containing IBA were analyzed to detect ß-carboline alkaloids by High Performance Thin Layer Chromatograghy (HPTLC).Harmine content was estimated to be 1.7 μg g −1 of the dried weight of transgenic hairy root cultures at the end of 50 days of culturing. The transformed roots induced by AR15834 strain, spontaneously, dedifferentiated as callus on MS medium without hormone. Optimum callus induction and shoot regeneration of transformed roots in vitro was achieved on MS medium containing 0.4 mg L −1 naphthaleneacetic acid and 2 mg L −1 6-benzylaminopurine (BAP) after 50 days. The main objective of this investigation was to establish hairy roots in this plant by using A. rhizogenes to synthesize secondary products at levels comparable to the wild-type roots.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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“…The most commonly used selectable marker gene for apple transformation is the neomycin phosphotransferase II (nptII) gene (De Bondt et al 1996;Puite and Shaart 1996;Holefors et al 1998Holefors et al , 2000Maximova et al 1998;Welander et al 1998;Wong et al 1999;Bolar et al 1999Bolar et al , 2000Liu et al 2001;Sedira et al 2001), which is responsible for the resistance against kanamycin. De Bondt et al (1996) also used the bar gene, but no transgenic ppt-resistant plants could be regenerated.…”

Section: Regeneration and Transformationmentioning

confidence: 99%

Transformation of apple ( Malus domestica Borkh.) with the stilbene synthase gene from grapevine ( Vitis vinifera L.) and a PGIP gene from kiwi ( Actinidia deliciosa )

Szankowski

Briviba²,

Fleschhut³

et al. 2003

Plant Cell Reports

133

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The objective of the present research was to introduce genes with antifungal potential into the commercially important apple cvs. Elstar and Holsteiner Cox in order to establish resistance against fungal diseases. The gene encoding the stilbene synthase (Vst1) from Vitis vinifera L., responsible for the synthesis of the phytoalexin resveratrol in grapevine, and the gene for a polygalacturonase-inhibiting protein (PGIP) from kiwi ( Actinidia deliciosa) were transferred into Holsteiner Cox and Elstar via Agrobacterium tumefaciens-mediated transformation. A total of nine transgenic Holsteiner Cox clones and one transgenic E clone carrying the stilbene-synthase gene as well as three transgenic Holsteiner Cox lines harbouring the polygalacturonase-inhibiting protein from Kiwi were identified via polymerase chain reaction and Southern blot analysis. High performance liquid chromatography analysis revealed the accumulation of a resveratrol-derivate, a glycoside, in transgenic Vst1 plants.

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“…Several reports concerning the transformation of apple (Malus x domestica Borkh.) using Agrobacterium tumefaciens are now available (De Bondt et al 1994;Maximova et al 1998;Welander et al 1998;Liu et al 2001;Zhu et al 2001;Igarashi et al 2002). However, literatures on genetics transformation of M. zumi are limited, and optimum conditions for shoot regeneration of M. zumi have not yet been elucidated.…”

mentioning

confidence: 99%

Efficient Agrobacterium tumefaciens-mediated transformation of Malus zumi (Matsumura) Rehd using leaf explant regeneration system

Xu¹,

Wang²,

Yin³

et al. 2009

Electron. J. Biotechnol.

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Genetic transformation of the apple rootstock M26 with the RolB gene and its influence on rooting

Cited by 60 publications

References 34 publications

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Transformation of apple ( Malus domestica Borkh.) with the stilbene synthase gene from grapevine ( Vitis vinifera L.) and a PGIP gene from kiwi ( Actinidia deliciosa )

Efficient Agrobacterium tumefaciens-mediated transformation of Malus zumi (Matsumura) Rehd using leaf explant regeneration system

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