Genetic Transformation of the Banana Hybrid Cultivar ‘FHIA-18’ (Musa sp.) via Particle Bombardment using Argon Gas

Daniels, Dion; Kosky, Rafael Gómez; Pérez, Laisyn Posada

doi:10.31695/ijasre.2018.32874

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…The cotyledons of B. campestris were bombarded once or twice to analyze the efficiency of Nluc expression. Several reports indicated that multiple bombardments affected gene transfer efficiency in various plant species [21][22][23]. Nluc expression induced by two bombardments was significantly lower than that induced by one bombardment (P = 0.0100, Fig.…”

Section: Effect Of the Number Of Bombardments In B Campestrismentioning

confidence: 96%

Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay

et al. 2023

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Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). Results We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.

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Section: Effect Of the Number Of Bombardments In B Campestrismentioning

confidence: 96%

Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay

et al. 2023

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“…Among these, ECS are the ideal explant because they are unicellular in origin, resulting in the production of stable nonchimeric plants for transgenic work (S agi et al, 2000). Various genetic transformation methods have been developed, such as electric shock (Sagi et al, 1994), biolistic bombardment transformation (Becker et al, 2000;Chee et al, 2005;Daniels et al, 2018;S agi et al, 1995;Vishnevetsky et al, 2011), Agrobacteriummediated transformation (Ganapathi et al, 2001;Ghag et al, 2012;Ghosh et al, 2009;Hu et al, 2013;Huang et al, 2007;Khanna et al, 2004;Kov acs et al, 2013;May et al, 1995;Remy et al, 2005;Shivani and Tiwari, 2019), and biolistic bombardment combined with Agrobacterium-mediated transformation (Liu et al, 2017). At present, genetic transformation of Agrobacterium-mediated ECS is the most widely used protocol because the problem of material browning during the transgenic period is abrogated by coculturing in a liquid medium, which significantly improves the yield of transgenic bananas (Ghosh et al, 2009;Huang et al, 2007).…”

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confidence: 99%

Highly Efficient Biolistic Transformation of Embryogenic Cell Suspensions of Banana Via a Liquid Medium Selection System

Dong¹,

Bi²,

Huang³

et al. 2020

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An efficient biolistic transformation system of banana combined with a liquid medium selection system was developed during this study. An embryogenic cell suspension (ECS) of Musa acuminata cv. Baxi (AAA) was bombarded with a particle delivery system. After 7 days of restoring culture in liquid M2 medium, embryogenic cells were transferred to a liquid selection M2 medium supplemented with 10 μg/mL hygromycin for resistance screening. The untransformed cell clusters were inhibited or killed, and a small number of transformants proliferated in the liquid selection medium. After the 0th, first, second, and third generation of antibiotic screening, there were 0, 65, 212, and 320, respectively, vitality-resistant buds obtained from a 0.5-mL packed cell volume (PCV) of embryogenic cell suspension. The β-glucuronidase (GUS) staining, polymerase chain reaction (PCR) analysis, and Southern blot hybridization results all demonstrated a 100% positive rate of regenerated resistant seedlings. Interestingly, the number of buds obtained through third-generation screening was almost equal to that obtained from the original ECS in M2 medium without antibiotics. These results suggested that the liquid medium selection system facilitated the proliferation of a positive transgenic ECS, which significantly improved the regeneration rate of transformants. This protocol is suitable for the genetic transformation of all banana genotypes and is highly advantageous to varieties with low callusing potential.

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Genetic Transformation of the Banana Hybrid Cultivar ‘FHIA-18’ (Musa sp.) via Particle Bombardment using Argon Gas

Cited by 2 publications

References 11 publications

Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay

Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay

Highly Efficient Biolistic Transformation of Embryogenic Cell Suspensions of Banana Via a Liquid Medium Selection System

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