Genetic transformation of the mycelium fungi Acremonium chrysogenum

Zhgun, A. A.; Ivanova, Mariya; Domracheva, A. G.; Novak, Metka; Elidarov, M. A.; Skryabin, K. G.; Bartoshevich, Yu. E.

doi:10.1134/s0003683808060070

Cited by 17 publications

(19 citation statements)

References 13 publications

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“…Microorganisms from initial samples grown on LB or CD agars or on mock layers were analyzed for texture, color of colonies, and hyphae morphology by macroscopic and microscopic observation as described previously with some modifications [18,19]. Light microscopy in native conditions was performed using a Carl Zeiss Jena microscope (Carl Zeiss, Germany) at ×1,000 magnification.…”

Section: Characterization Of Microorganisms In Initial Samples Cultumentioning

confidence: 99%

Detection of potential biodeterioration risks for tempera painting in 16th century exhibits from State Tretyakov Gallery

Zhgun¹,

Avdanina²,

Shumikhin

et al. 2020

PLoS ONE

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In this study, we investigated biodeterioration of materials used in tempera painting by analyzing the structure of the microbiome in ancient tempera paintings exhibited in State Tretyakov Gallery, Moscow, Russia. Samples were obtained from 16 th-century paintings, including a grand Russian Orthodox icon "The Church Militant" (all exhibits were without visible signs of biodeterioration), and from surrounding walls and ceilings (with vast zones of visible microbial growth). A number of microorganisms isolated from visible signs of environmental bio-damage were also detected in tempera paintings kept in temperature-and humidity-controlled conditions unfavorable for the growth of microflora. To determine the biodegrading potential of the microbiome for tempera paintings, we developed a set of mock layers from paintwork materials used in tempera painting of 16 th century and their modern analogues and inoculated them with cultures containing filamentous fungi and bacteria. The susceptibility to microbial degradation of individual tempera painting materials was examined by micro-Fourier Transform Infrared (FTIR) spectroscopy, which enabled detection of even invisible signs of biodeterioration. The results indicate that the microorganisms isolated from paintings and surrounding areas in the museum are capable of causing significant damage of various tempera materials, among which varnishes were the most resistant; however, the addition of antiseptic (sodium pentachlorophenolate) can inhibit microbial growth on sturgeon glue.

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Section: Characterization Of Microorganisms In Initial Samples Cultumentioning

confidence: 99%

Detection of potential biodeterioration risks for tempera painting in 16th century exhibits from State Tretyakov Gallery

Zhgun¹,

Avdanina²,

Shumikhin

et al. 2020

PLoS ONE

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“…The addition of 5 mM α-difluoromethylornithine (DFMO) or 5 mM of 1-aminooxy-3-aminopropane (APA) completely inhibited the growth of the WT strain, unlike HY strain (Figure 4) [65]. Such kind of resistance against inhibiters of key enzyme for PAs production turned out to be rather strange since HY strain is significantly weakened after SCI program [32,61,64,66,67]. The only previously observed advantage over the WT strain was expressed in CPC overproduction [41].…”

Section: The Endogenous Polyamines Content In a Chrysogenum Hy Strainmentioning

confidence: 99%

“…However, along with beneficial changes that increase the production of the target SM, reduce the amount of spin-off products, and others, numerous side changes begin to accumulate in the fungal strain. They can appear in a slow growth on agar and liquid media [27,64,65], a decrease in stress resistance [66], reduction in the conidia formation [64] and many other properties, expressed in a decrease in the overall viability of the strain [66,67]. Finally, the stage comes when the next mutagenic effect no longer leads to further strain improvement.…”

Section: The Technological Limit Of Csi Of Filamentous Fungi For Sm Pmentioning

confidence: 99%

Random Mutagenesis of Filamentous Fungi Strains for High-Yield Production of Secondary Metabolites: The Role of Polyamines

Жгун¹

2021

Genotoxicity and Mutagenicity - Mechanisms and Test Methods

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A filamentous fungus (also called molds or moldy fungus) is a taxonomically diverse organism from phylum Zygomycota and Ascomycota with filamentous hyphae and has the ability to produce airborne spores or conidia. Currently, more than 70,000 molds are known, and some of them contain unique and unusual biochemical pathways. A number of products from such pathways, especially, the secondary metabolite (SM) pathways are used as important pharmaceuticals, including antibiotics, statins, and immunodepresants. Under different conditions, the individual species can produce more than 100 SM. The strain improvement programs lead to high yielding in target SM and significant reduction of spin-off products. The main tool for the strain improvement of filamentous fungi is random mutagenesis and screening. The majority of industrial overproducing SM strains were developed with the help of such technique over the past 50–70 years; the yield of the target SM increased by 100- to 1000-fold or more. Moreover, most of the strains have reached their technological limit of improvement. A new round of mutagenesis has not increased overproduction. Recently, it was shown that that the addition of exogenous polyamines may increase the production of such improved strains of filamentous fungi. The possible molecular mechanism of this phenomenon and its biotechnological applications are discussed.

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“…Two strains of Acremonium chrysogenum were used in this study: the wild-type (Brotzu) isolate ATCC 11550 (WT) [36] and high-cephalosporin-yielding RNCM F-4081D (HY), derived from the WT [37]. The cultivation conditions were the same as previously described [38,39]. The filamentous fungi were cultivated on agarized Czapek-Dox (CD) medium (30 g/L sucrose, 2 g/L NaNO 3 , 1 g/L K 2 HPO 4 , 0.5 g/L MgSO 4 ×7 H 2 O, 0.5 g/L KCl, 0.01 g/L FeSO 4 ×7 H 2 O, 20 g/L agar, pH 7.0-7.4).…”

Section: Fungal Growth Inhibitionmentioning

confidence: 99%

Hydroxylamine Analogue of Agmatine: Magic Bullet for Arginine Decarboxylase

et al. 2020

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The biogenic polyamines, spermine, spermidine (Spd) and putrescine (Put) are present at micro-millimolar concentrations in eukaryotic and prokaryotic cells (many prokaryotes have no spermine), participating in the regulation of cellular proliferation and differentiation. In mammalian cells Put is formed exclusively from L-ornithine by ornithine decarboxylase (ODC) and many potent ODC inhibitors are known. In bacteria, plants, and fungi Put is synthesized also from agmatine, which is formed from L-arginine by arginine decarboxylase (ADC). Here we demonstrate that the isosteric hydroxylamine analogue of agmatine (AO-Agm) is a new and very potent (IC50 3•10−8 M) inhibitor of E. coli ADC. It was almost two orders of magnitude less potent towards E. coli ODC. AO-Agm decreased polyamine pools and inhibited the growth of DU145 prostate cancer cells only at high concentration (1 mM). Growth inhibitory analysis of the Acremonium chrysogenum demonstrated that the wild type (WT) strain synthesized Put only from L-ornithine, while the cephalosporin C high-yielding strain, in which the polyamine pool is increased, could use both ODC and ADC to produce Put. Thus, AO-Agm is an important addition to the set of existing inhibitors of the enzymes of polyamine biosynthesis, and an important instrument for investigating polyamine biochemistry.

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Genetic transformation of the mycelium fungi Acremonium chrysogenum

Cited by 17 publications

References 13 publications

Detection of potential biodeterioration risks for tempera painting in 16th century exhibits from State Tretyakov Gallery

Detection of potential biodeterioration risks for tempera painting in 16th century exhibits from State Tretyakov Gallery

Random Mutagenesis of Filamentous Fungi Strains for High-Yield Production of Secondary Metabolites: The Role of Polyamines

Hydroxylamine Analogue of Agmatine: Magic Bullet for Arginine Decarboxylase

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