Genetic transformation ofCatharanthus roseus G. Don byAgrobacterium rhizogenes

Abstract

Catharanthus roseus plantlets were inoculated with differentAgrobacterium rhizogenes strains. This plant species is known to produce secondary metabolites and axenic hairy-root cultures are an alternative to extraction of plant tissue for the compounds which are synthesized in roots. Hairy root lines were established from inoculations with the agropine strain 15834 and transformed plants were obtained after spontaneous regeneration. Phenotypic alterations of both the root system and the aerial parts were observed in transformed plants. All the tissues analyzed contained agropine and mannopine. T-DNA analysis confirmed the presence of TL- and TR-DNAs either as distinct inserts, or as a single and continuous insert including the region between TL and TR on pRi 15834.

Enhanced accumulation of catharanthine and vindoline in Catharanthus roseus hairy roots by overexpression of transcriptional factor ORCA2

“…Therefore, many cell and hairy root cultures produce catharanthine and tabersonine, but do not produce vindoline because there is a limitation in the conversion from tabersonine to vindoline (Shanks et al, 1998). This fact had been confirmed by some experiments (Bhadra and Shanks, 1997;Brillanceau et al, 1989;Toivonen et al, 1989).…”

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

“…Reports are available from earlier studies also that A. rhizogenes strain 15834 is most effective strain for hairy root development in Rubia tinctorum and Catharanthus roseus. (Brillanceau et al 1989;Ercan et al 1999). One of the important findings of the current study was the emergence of hairy roots from transgenic explants on medium without adding PGRs.…”

Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri�T-DNA gene integration

“…Agrobacterium rhizogenes, a gram-negative soil bacterium, is well known for having the ability to transfer its T-DNA from the root-inducing (Ri) plasmid to the host genome and thereby causing hairy root disease of plants (Chilton et al 1982;Brillanceau et al 1989). A. rhizogenes Ri plasmids have been assigned to three classesmannopine, cucumopine and agropine-depending on the opine synthesized and degraded.…”