Genetic Transformation on Orchid Species: An Overview of Approaches and Methodologies

Mii, Masahiro; Chin, Dong Poh

doi:10.1007/978-1-4939-7771-0_19

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…PLBs can be also used for transformation protocols and successful protocols were developed and obtained stable transgenics with target characteristics for floriculture [146,147]. In genetic transformation of orchids, the use of PLBs derived directly from individual epidermal cells resulted in solid transgenic plants with clonal identity of Oncidium Sharry Baby 'OM8' [32], an exceptional advantage over PLBs from callus and with multicellular origin [126], which may result in the emergence of somaclonal variants [42] and chimeric tissues when used for genetic transformation, which are difficult to characterize and separate [32].…”

Section: Applications Of Ipr-plb Technique On Orchid Propagation and mentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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The process through induction, proliferation and regeneration of protocorm-like bodies (PLBs) is one of the most advantageous methods for mass propagation of orchids which applied to the world floricultural market. In addition, this method has been used as a tool to identify genes of interest associated with the production of PLBs, and also in breeding techniques that use biotechnology to produce new cultivars, such as to obtain transgenic plants. Most of the molecular studies developed have used model plants as species of Phalaenopsis, and interestingly, despite similarities to somatic embryogenesis, some molecular differences do not yet allow to characterize that PLB induction is in fact a type of somatic embryogenesis. Despite the importance of species for conservation and collection purposes, the flower market is supported by hybrid cultivars, usually polyploid, which makes more detailed molecular evaluations difficult. Studies on the effect of plant growth regulators on induction, proliferation, and regeneration of PLBs are the most numerous. However, studies of other factors and new technologies affecting PLB production such as the use of temporary immersion bioreactors and the use of lighting-emitting diodes have emerged as new tools for advancing the technique with increasing PLB production efficiency. In addition, recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation, such as somaclonal variation.

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Section: Applications Of Ipr-plb Technique On Orchid Propagation and mentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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“…The different orchid species show great variations in physiological and morphological features, requiring different conditions for growth in plant tissue culture. Different species respond differently to growth factors (depending on the genotype), and some are recalcitrant in in vitro conditions [76]. In terrestrial orchids, such as Paphiopedilum and Cypripedium, meristem explant survival and PLB induction are difficult.…”

Section: In Vitro Orchid Propagation In Plant Tissue Culturementioning

confidence: 99%

“…In addition, a method developed for a particular species may not work for another species. Therefore, for targeted genetic transformation, a plant regeneration protocol must be developed for a particular plant genotype [76], and the identification of variations in genotype must occur before plant transformation is necessary. The subsequent discovery of asymbiotic seed germination (for in vitro plant propagation) has been widely employed to develop orchid plants in vitro and direct somatic embryogenesis [77], totipotent callus induction, shoot-bud formation from different explants, PLB formation from cell suspension culture, and others, which have immensely contributed to the micropropagation and creation of transgenic hybrids [5].…”

Section: In Vitro Orchid Propagation In Plant Tissue Culturementioning

confidence: 99%

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Tiwari,

Sharma,

Bose

et al. 2024

Horticulturae

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Orchids constitute the largest and most diverse group of flowering plants and are classified in the family Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plant, the cultivation of orchids on a commercial level is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has successfully generated employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably bridged the knowledge gaps in areas of orchid classification, phytochemistry, and cultivation strategies. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, and their global marketing as cut flowers and artificially propagated plants. Orchids constitute the first floriculture crops that revolutionized the orchid industry; however, they also hold several challenges in the natural propagation and conservation of several species that are on the verge of extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming for conservation and legal commercial goals. This thematic review is one-of-a-kind in providing comprehensive insights into the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming times. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach to the conservation and commercialization of orchids.

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Protocorm-Like Bodies Regeneration from Callus Cultures of Phalaenopsis

Hsu,

Huang

2024

Springer Protocols Handbooks

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Genetic Transformation on Orchid Species: An Overview of Approaches and Methodologies

Cited by 5 publications

References 38 publications

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Protocorm-Like Bodies Regeneration from Callus Cultures of Phalaenopsis

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